Evaluation of Chemopreventive and Analgesic Activity of Hibiscus cannabinus L. Seed Oil in Mice doi.org/10.26538/tjnpr/v5i4.7
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Abstract
Hibiscus cannabinus L., a useful edible plant from Malvaceae family is familiar as Kenaf. The study was aimed to evaluate the chemopreventive and analgesic potentials of Kenaf seeds oil (KSO) using skin carcinogenic mice where carcinogenesis was induced by 7, 12-dimethylbenz (a) anthracene (DMBA) and croton oil. KSO was administered orally at two different doses (250 and 500 mg/kg) from two weeks before the DMBA application and continued till the end of the experiment. Analgesic activity was assessed using the acetic acid-induced algesia in mice. Administration of KSO significantly suppressed the tumor incidence, tumor yield and tumor burden in comparison with carcinogen control group. The number of tumors, tumor diameter and tumor weight were comparatively lower in KSO-treated groups. Moreover, the biochemical analysis of tumor homogenate showed a significant elevation of reduced glutathione (GHS) levels in oil-treated mice in comparison with carcinogen control mice. These results support the cancer preventive potential of KSO. In addition, the seed oil showed 30.30% and 47.74% writhing suppression at the dose of 250 and 500 mg/kg respectively in acetic acid-treated mice whereas 76.13% suppression was observed with diclofenac sodium (25 mg/kg).
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