Phytochemical Screening of Solenostemon monostachyus and the Effect of Extract and Fractions on Castor Oil-Induced Diarrhoea in Rats doi.org/10.26538/tjnpr/v5i4.6
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Abstract
Solenostemon monostachyus P. Beauv (Lamiaceae) is among plants reportedly used in West and Central African ethnomedicine for the treatment and management of diabetes, malaria, hypertension and sickle cell anaemia. The effect of ethanol leaves extract and fractions (chloroform and aqueous) from Solenostemon monostachyus on castor oil-induced diarrhoea was studied in rats. The extract (75-225 mg/kg) and fractions (150 mg/kg) were evaluated for their effect on experimentally induced diarrhoea using different models; castor oil-induced diarrhoea, castor oil-induced fluid accumulation and small intestinal propulsion. The extract inhibited significantly (p < 0.05) diarrhoea induced by castor oil in a non-dose-dependent fashion. The extract (150 mg/kg) and aqueous fraction (150 mg/kg) showed considerably higher inhibition of castor oil-induced diarrhoea, small intestine transit time and castor oil induced fluid accumulation in vivo although the effect was not comparable to the standard drugs, Loperamide and Atropine. Results of phytochemical screening showed the presence of alkaloids, saponins, tannins and cardiac glycosides. The observed antidiarrhoeal property of the ethanol leaves extract of the plant may be linked to the presence of these phytoconstituents.
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References
Njume C and Goduka NI. Treatment of diarrhoea in rural African communities: an overview of measures to maximize the medicinal potentials of indigenous plants”. Int J Environ Res Pub Health. 2012; 9(11):3911-3933.
World Health Organisation (WHO). Diarrhoea. Why children are still dying and what can be done. Tutto, Geneva, Switzerland.2009.
Amaechi D, Udosen EO, Edet EE, Asanga EE, Mbakwe IE. Lipid profile status of streptozotocin induced diabetic rats treated with ethanolic leaf extract of Solenostemon monostachyus. J Med Plant Res. 2015; 9(8):289-293.
Abdulhalin, A and Hanrahan J. Biologically active compounds from Lamiaceae family: Central nervous system effects. Stud Nat Prod Chem. 2021; 68:255-315.
Mve-Mba CE, Monut C, Lamaty G, Zollo PHA, Tchoumbougnang. Aromatic plants of tropical central Africa Part XIX Volatile components from leaves of two lamiaceaefrom Cameroon: Leucas deflexa Hook and Solenostemon monostachyus (P Beauv). Briq Flav Fragr J. 1994; 9:315-317.
Adebayo JO and Krettli AU. Potential antimalarials from Nigerian plants: A review. J Ethnopharmacol. 2011; 133:289-302.
Ajibesin KK, Ekpo BA, Bala DN, Essien EE, Adesanya SA. Ethnobotanical survey of Akwa Ibom State of Nigeria. J Ethnopharmacol. 2008; 115:387-408.
Koffi N, Marie –Solange T, Emma AA, Noel ZG. Ethnobotanical study of plants used to treat arterial hypertension in traditional medicine, by Abbey and Krobou population of Agboville (Cote d’ivoire). Eur J Sci Res. 2009; 35:85-98.
N’Guessan HA, Dago DCE, Mamyrbekova-Bekro JA, Békro YA. CCM d’extraits selectifs de 10 plantes utilisées dans le traitement traditionnel de l’hypertension arterielle n Côte d’Ivoire. Eur J Sci Res. 2011; 66:575-585.
Okoko T and Ere D. Antioxidant activities of Solenostemon monostachyus leaf extract using in vitro methods. Sci Res Essays. 2012; 7:621-626.
Fidele KZ, Andre KB, Yao DJ, Michel OA. Action of hydroethanolic leaves extract of Solenostemon monostachyus(Lamiaceae) on cardiovascular system of mammalians: blood pressure lowering effects. Int J Pharm Biol Sci. 2012; 2:310-320.
Ekundayo EO and Ezeogu LI. Evaluation of antimicrobial activities of extracts of five plants used in traditional medicine in Nigeria. Int J Trop Med. 2006; 1:93-96.
Amazu LU, Antia BS, Okokon JE. Antiulcer activity of S monostachyus. The J Phytopharmacol. 2015; 4:97-101.
Okokon JE, Amazu LU, Nwidu LL. Antidiabetic and hypolipidemic activities of Solenostemon monostachyus. J Herbal Drugs. 2015; 6(2):83-90.
Okokon JE, Davis K, Azare B. Antimalarial and antipyretic activities of Solenostemon monostachyus. Pharm Biol. 2016a; 54:648-653.
Okokon JE, Davis K, Nwidu LL. Anti-inflammatory and antinociceptive activities of Solenostemon monostachyus aerial part extract in mice. Aviccena J Phytomed. 2016b; 6(3):284-294.
Okokon JE, Oguamanam W, Umoh EE. Hepatoprotective and nephroprotective activities of Solenostemon monostachyus leaf extract. Afr J Pharmacol Ther. 2017; 6(2):123-133.
Okokon JE, Kufre D, Bassey, AI. Psychopharmacological Studyof Ethanol Leaf Extract of Solenostemon monostachyus. Nig J Pharm Appl Sci Res. 2020; 9(3):59-63.
Toshio M, Peter R, Conrad HE. Structures of six coleons (diterpenoids) from Solenostemon monostachyus (P. Beauv.) Briq. (Labiatae). Helv Chim Acta. 1980; 63:95-101.
Harborne JB. Photochemical methods, a Guide to modern Techniques in plant Analysis. Champion and Hall. London. 1973; 279 p.
Trease GE and Evans WC. Textbook of Pharmacognosy.14thEdition. W.B. Sanders, London. 1989; 14.
Sunil B, Bedi K, Singla A, Johri R. Antidiarrhoeal activity of piperine in mice. Planta Med. 2001; 67:284-287.
Nwafor PA, Jacks TW, Ekanem AU, Ching FP. Antiulcerogenic and antidiarrhoeal potentials of Pausinystalia macroceras stembark in rats. NJNPM, 2005; 9:66-70.
Dicarlo GD, Mascolo N, Izzo AA, Capasso F, Autore G. Effects of Quercetin on gastrointestinal tract in rats and mice. Phytother Res. 1994; 8:42-45.
Galvez J, Zarzuelo A, Crespo ME, Lorente MD, Ocente MA and Jimenez J. Antidiarrhoeic activity of Euphorbia hirta extract andisolation of an active flavonoid constituent. Planta Med. 1993; 59:333-336.
Yoshio K, Kazuko S, Bunsyo M, Kazunori H, Atsushi I and Yasuhiro K. Relationship between antidiarrhoeal effects of Hange-shashin-To and active components. Phytother Res. 1999; 13:468-473.
Rouf AS, Islam MS, Rahman MT. Evaluation of antidiarrhoeal activity of Rumex maritimus roots. J Ethnopharmacol. 2003; 84:307-310.
Okokon JE, Akpan HD, Umoh EE, Itemobong, Ekaidem IS. Antidiarrhoeal and antiulcer activities of Hippocratea africanaroot extract. Pak J Pharm Sci. 2011; 24:201-205.
Vareinshang T and Yadav AK. Antidiarrhoeal activity of Rhus javanica ripen fruit extract in albino mice. Fitoter. 2004; 75:39-44.
Okwu DE. Phytochemicals and Vitamins content of indigenous species of South Eastern Nigeria. J Sustain Agric Environ. 2004; 6:30-34.
Adinortey MB, Sarfo JK, Quayson, ET, Weremfo A, Adinortey CA, Ekloh W, Oeran J. Phytochemical screening, Proximate and Mineral composition of Launaea teraxacifolia leaves. Res J Med Plant. 2012; 2(1):171-179.
Amin MM, Sawhne SS, Jassal MMS. Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker J Pham Phamacol. 2012; 2:001-005.
Abigail J, Lararson J, David S, Thunder J. Therapeutic potential of quercetin to decrease blood pressure. Adv Nutr. 2012; 3:39-46.
Oyeyemi SD, Arowosegbe S, Famosa MA. Phytochemical Constituents and Nutritional Evaluation of Three Selected Edible Flowers in Ado-Ekiti, Nigeria. ChemSearch J. 2017; 8(1):44-48.
Havagiray R, Ramesh C and Sadhna K. Study of antidiarrhoeal activity of Calotropis gigantea r.b.r. in experimental animals. J Pharm Pharm Sci. 2004; 7:70-75.