In vitro Erectogenic Property of Aqueous Extract from Roasted and Raw Pepitas in Isolated Corpora cavernosa of Wistar Rats
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Abstract
Pepitas have long been adopted in traditional medicine to to help with erectile dysfunction (ED) and cure impotence in males. This study investigated the inhibitory potentials of raw and roasted pepitas on some of the main enzymes associated with erectile dysfunction in the corpora cavernosa of rats. Arginase, Phosphodiesterase-5 (PDE-5), acetylcholinesterase (AChE), angiotensin I-conversion enzyme (ACE), and adenosine deaminase (ADA) were among the enzymes studied. The raw and roasted pepitas water extract (1:10 w/v) was employed. Gas chromatography-Pulsed Flame Photometric Detector (GC-PFPD) was also used to assess the amino acid content of roasted and raw pepitas. In the rat's corpora cavernosa, the roasted extract exhibited higher inhibitory activities for PDE-5 (IC50 = 15.76 g/mL), ACE (IC50 = 37.29 g/mL), AChE (IC50 = 28.34 g/mL), arginase (IC50 = 44.86 g/mL), and ADA (IC50 = 15.10 g/mL). The roasted extract had a lot of arginine, while the raw extract didn't have any. Pepitas' enzyme activity and erectogenic qualities might be linked to their amino acid contents. The pepitas that had been roasted seemed to be the most promising.
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