Determination of a Chemical Marker in Dipterocarpus alatus Oleoresin Samples and Bioactivity Screening via Antioxidants, Nitric Oxide Inhibition on Murine RAW 264.7 Cells, and Collagen Production on Normal Human Dermal Fibroblasts doi.org/10.26538/tjnpr/v5i5.10
Main Article Content
Abstract
Oleoresin from the many species of Dipterocarps consist of several group of compounds such as monoterpenes and diterpene resin acids, sesquiterpenes and essential oils which presented various bioactivities. In this research, the 5 samples of D. alatus oleoresin from the different sources and preparation methods were evaluated for the chemical contents and biological activities. The oleoresin samples obtained from Roi Et province, Thailand (OD1), heating oleoresin (OD2), supernatant and precipitate after degumming oleoresin (OD3 and OD4) and the oleoresin from Surat Thani Province, Thailand (OD5) were determined for total phenolic compounds (TPC) and (-)-α-gurjunene using validated HPLC-UV technique. The biological activities such as, antioxidation, inhibition of nitric oxide (NO) production on murine RAW 264.7 cells, and collagen production activity on normal human dermal fibroblasts (NHDFs) were also studied. The results indicated that the major component of all oleoresin samples was (-)-α- gurjunene. OD5 established the highest (-)-α-gurjunene content and TPC. All samples demonstrated the potential to inhibit NO production on RAW 264.7 cells comparable to positive control (L-NAME), OD3 showed the highest activity with the NO inhibition as 71.89%. In addition, the OD2 could stimulate higher collagen production on NHDFs than positive, ascorbic acid. Therefore, different oleoresin preparation methods and different sources affected the chemical contents and biological activities which are nitric oxide inhibition and collagen production. Our results suggested that D. alatus oleoresin has potential to be developed as an anti-inflammatory or wound healing product(s), however, its efficacy requires further investigation.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Ali B, Al-Wabel NA, Shams S, Ahamad A, Khan SA, Shahzadi I, Nadeem R, Hanif MA, Mumtaz S, Jilani I, Nisar S. Chemistry and biosynthesis pathways of plant oleoresins: Important drug sources. Int J Biol Chem Sci. 2017; 12:18-52.
Appanah S and Turnbull JW. A review of dipterocarps: taxonomy, ecology and silviculture. Bogor, Indonesia: Center for International Forestry Research (CIFOR); 1998; 220p.
Fernandes A and Maharani R. Phytochemical and GC-MS analysis of oleoresin of dipterocarpus gracilis: blume: as a basic consideration for human remedy. Int J Pharm Sci Res. 2019; 10(5):2224-2229.
Sri Rama Murthy K, Lakshmi N, Ramulu D. Biological activity and phytochemical screening of the oleoresin of Shorea robusta: Gaertn. f. Trop Subtrop Agroecosystems. 2011; 14:787-791.
Yongram C, Sungthong B, Puthongking P, Weerapreeyakul N. Chemical composition, antioxidant and cytotoxicity activities of
leaves, bark, twigs and oleo-resin of Dipterocarpus alatus. Molecules. 2019; 24(17):3083-3092.
da Silva Rodrigues-Corrêa KC, de Lima JC, Fett-Neto AG. Oleoresins from pine: production and industrial uses. In: Natural
Products: Phytochemistry, botany and metabolism of alkaloids, Phenolics and Terpenes. Berlin, Heidelberg: Springer; 2013.
-4060 p.
Poojeera S, Worawong T, Areesinpitak T, Katekaew S. Comparison and evaluation the fuel quality of degummed oleoresin of Yang-na oil. UDRU Sci Technol J. 2017; 5(1):9-23.
Khunchalee J and Roschat W. The Study of physicochemical properties of the Yang – Na (Dipterocarpus Alatus): oil for use as a high potentiality feedstock to produce liquid biofuel in Thailand. J Mater Sci Appl Energ. 2020; 9(2):522-530.
Gushiken LFS, Hussni CA, Bastos JK, Rozza AL, Beserra FP, Vieira AJ, Padovani, CR, Lemos, ,M, Polizello Junior, M, da Silva, JJM, Nóbrega, RH, Martinez, ERM, Pellizzon, CH. Skin wound healing potential and mechanisms of the hydroalcoholic extract of leaves and oleoresin of Copaifera langsdorffii: Desf. Kuntze in rats. Evid-Based Compl Altern Med. 2017; 2017(70):1-16.
Masson-Meyers D, Andrade T, Leite S, Frade M. Cytotoxicity and wound healing properties of Copaifera langsdorffii:oleoresin in rabbits. Int J Nat Prod Sci. 2013; 3:10-20.
Masson-Meyers D, Enwemeka CS, Bumah V, Andrade T, Frade MA. Topical treatment with Copaifera langsdorffii: oleoresin improves wound healing in rats. Int J Phytomed. 2013; 5(3):378-386.
Windyaswari AS, Purba JP, Nurrahmah SS, Ayu IP, Imran Z, Amin AA, Kurniawanet, F, Pratiwi NTM, Iswantari A. Phytochemical profile of sea grass extract (Enhalus acoroides): A new marine source from Ekas Bay, East Lombok. IOP Conf Ser: Earth Environ Sci. 2019; 278:1-9.
ICH Q2B. Guidance for Industry Q2B Validation of Analytical Procedures: Methodology. ICH; 1996.
Ghafoor K, Al Juhaimi F, Özcan MM, Uslu N, Babiker EE, Mohamed Ahmed IA. Total phenolics, total carotenoids, individual phenolics and antioxidant activity of ginger (Zingiber officinale): rhizome as affected by drying methods. LWT. 2020; 126:1-7.
Le HT, Luu TN, Nguyen HMT, Nguyen DH, Le PTQ, Trịnh NN, Le,VS, Nguyen, HD, Van, HT. Antibacterial, antioxidant and cytotoxic activities of different fractions of acetone extract from flowers of Dipterocarpus intricatus: Dyer (Dipterocarpaceae). Plant Sci Today. 2021; 8(2):273-277.
Lulan TYK, Fatmawati S, Santoso M, Ersam T. α-VINIFERIN as a potential antidiabetic and antiplasmodial extracted from Dipterocarpus littoralis: Heliyon. 2020; 6(5):1-6.
Jorge LF, Meniqueti AB, Silva RF, Santos KA, Da Silva EA, Gonçalves JE, De Rezende, CM, Colauto, NB, Gazim, ZC, Linde, GE. Antioxidant activity and chemical composition of oleoresin from leaves and flowers of Brunfelsia uniflora: Genet Mol Res. 2017; 16(3):1-13.
Tung Y-T, Yen P-L, Lin C-Y, Chang S-T. Anti-inflammatory activities of essential oils and their constituents from different provenances of indigenous cinnamon (Cinnamomum osmophloeum:) leaves. Pharm Biol. 2010; 48(10):1130-1136.
Jin M, Suh S-J, Yang JH, Lu Y, Kim SJ, Kwon S, Park, YI, Ahn, GW, Lee, CK, Kim, CH, Son, JK, Son, KH, Chang, HW. Antiinflammatory activity of bark of Dioscorea batatas: DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-κB and ERK1/2 inactivation. Food Chem Toxicol. 2010; 48(11):3073-3079.
Toledo-Piza AR, Nakano E, Rici REG, Maria DA. Proliferation of fibroblasts and endothelial cells is enhanced by treatment with
Phyllocaulis boraceiensis: mucus. Cell Prolif. 2013; 46(1):97-108.
Taşkiran D, Taşkiran E, Yercan H, Kutay FZ. Quantification of total collagen in rabbit tendon by the Sirius Red Method. Turk J Med Sci. 1997; 29:7-9.
Sharma YC, Yadav M, Upadhyay SN. Latest advances in degumming feedstock oils for large-scale biodiesel production. Biofpr. 2019; 13(1):174-191.
Lalas S and Tsaknis J. Extraction and identification of natural antioxidant from the seeds of the Moringa oleifera: tree variety of Malawi. J Amer Oil Chem Soc. 2002; 79(7):677-683.
Farghadan M, Ghafoori H, Vakhshiteh F, Fazeli SAS, Farzaneh P, Kokhaei P. The effect of Artemisia fragrans: Willd: Essential oil on inducible nitric oxide synthase gene expression and nitric oxide production in lipopolysaccharide-stimulated murine macrophage cell line. Iran J Allergy Asthma Immunol. 2016; 15(6):515-524.
Morikawa T, Matsuda H, Yoshikawa M. A Review of Antiinflammatory Terpenoids from the Incense Gum Resins Frankincense and Myrrh. J Oleo Sci. Sci. 2017; 66(8):805-814.
de Lavor ÉM, Fernandes AWC, de Andrade Teles RB, Leal AEBP, de Oliveira Júnior RG, Gama e Silva M, et al. Essential oils and their major compounds in the treatment of chronic inflammation: A review of antioxidant potential in preclinical studies and molecular mechanisms. Oxid Med Cell Longev. 2018; 2018:e6468593.
Amilia Destryana R, Gary Young D, Woolley CL, Huang T-C, Wu H-Y, Shih W-L. Antioxidant and Anti-inflammation Activities of Ocotea, Copaiba and Blue Cypress Essential Oils in Vitro and in Vivo. J Am Oil Chem Soc. 2014; 91(9):1531-1542.
Yoon M-S, Won K-J, Kim DY, Hwang D il, Yoon SW, Kim B, Lee HM. Skin regeneration effect and chemical composition of
essential oil from Artemisia montana: Nat Prod Commun. 2014; 9(11):1619-1622.
Mazutti da Silva SM, Rezende Costa CR, Martins Gelfuso G, Silva Guerra EN, de Medeiros Nóbrega YK, Gomes SM, et al. Wound healing effect of essential oil extracted from Eugenia dysenterica: DC (Myrtaceae) leaves. Molecules. 2018; 24(1):1-16.