GC-MS Analysis of Essential Oil Composition and Insecticidal Activity of Syzygium aromaticum against Callosobruchus Maculatus of Chickpea doi.org/10.26538/tjnpr/v5i5.9
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Abstract
The beetle Callosobruchus maculatus (Chrysomelidae: Bruchinae) is a destructive pest of stored chickpea seeds. The use of plant materials rather than conventional pesticides to control these pests is a promising alternative to chemical insecticides. The present study evaluated the insecticidal activity of clove essential oils by three different methods on Callosobruchus maculatus. The oils were obtained by hydro-distillation with a yield of 6.3 ± 0.23% and analyzed by GC-MS. In total, 65 compounds representing 99.92% of the oil were identified and the main components 80.26% Eugenol, 9.62% Eugenyl acetate, 6.74% β-Caryophyllene and 1.14% C-Humulene. The results of the insecticidal activity of S. aromaticum on C. maculatus performed by inhalation shows that a significant mortality was observed at a concentration of 20 μl/l against insects exposed at 24 hours (6.67%), 48 hours (36.67%) and 72 hours (100%).Contact test results are lower than those produced by inhalation. Essential oil decreased oviposition and fully suppressed adult growth at the highest dose of 20 μl/l of air per contact and inhalation test. The results of the present research have shown that EO had a high effectiveness against Callosobruchus maculatus, and in another hand these results can be used as a source of natural product to avoid the contamination of chickpea seeds, and also as an alternative to chemical products.
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