Ameliorative potentials of Linum usitatissimum (Linn) (Flax seed) Crude Extract Against Lead Acetate-induced Neuronal Damage on the Hippocampal Histoarchitecture Using Wistar rat Model
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Abstract
Lead-induced neurotoxicity is on the increase and the search for herbal remedy over orthodox intervention is also gaining popularity. The study investigated the ameliorative properties of flaxseed crude extract against lead acetate-induced neuronal damage in the hippocampus of Wistar rat. Thirty adult male Wistar rats (100 – 120 g) were used and assigned randomly into five groups A-E (n=6). Group A is the control, Group B was the negative control, while Groups C, D, E were test groups. Lead acetate was administered at a dose of 5 mg/kg for 14 days, Dimercaptosuccinic acid (DMSA) and flaxseed were later administered for 14 days. Neurobehavioral test (Radial arm maze) was carried out after administration. At the end of the experiment, the animals were sacrificed using cervical dislocation. Hippocampal tissues were collected after animal sacrifice and analyzed for glucose-6-phosphate dehydrogenase activity, Malondialdehyde (MDA), gamma-aminobutyric acid (GABA) levels, and the immunohistochemical expression of Tumor Necrosis Factor α (TNF-α) using enzyme tests. Data collected were analysed using one-way ANOVA, followed by Tukey post hoc for multiple comparisons. Flax seed extract reversed the neuronal damage and spatial memory deficit caused by lead acetate exposure to the hippocampus. The study concluded that 200 mg/kg of Linum usitatissimum crude extract had an ameliorative effect against memory impairment caused by lead acetate in rat model.
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