Genotyping and Phytochemical Analysis of Three Species of Spider Lily (Hymenocallis spp.)
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Abstract
Spider lily is a plant that contains phytochemicals for various pharmacological activities. Morphological similarities in several spider lily species make it difficult to identify morphologically. Molecular approaches are recommended for identifying plant species that are difficult to identify morphologically. This study aimed to identify three species of spider lily (Hymenocallis spp.) in Denpasar, Bali (Flat Lily, Bangkok Lily, and Small Leaf Lily) using DNA barcoding. The phytochemical of the extract was identified using Gas Chromatography-Mass Spectrometry (GC-MS). The molecular data showed that the three species were genetically close to Hymenocallis littoralis, although significant genetic distance was still found. Based on phylogenetic tree construction using the Maximum Likelihood method, the three species in Denpasar are in the same clade as Hymenocallis littoralis, Hymenocallis maximiliani, and Hymenocallis caribaea but still show some relevant genetic variation. Various compounds were identified in the leaf extracts. 2,4,6-Cycloheptatrien, Trehalose and Hydroxymethylfurfural were bioactive compounds with high AUC known with known antioxidant, anti-inflammatory, and neuroprotective potential.
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