Green Synthesized Zinc Oxide Nanoparticles from Coffea arabica: Bioprospecting and Functional Potential as an Antioxidant and Larvicidal Agent against Aedes aegypti

Article Sidebar

pdf epub
Published: Jan 31, 2025
DOI: https://doi.org/10.26538/tjnpr/v9i1.13
Keywords:
Aedes aegypti, Larvicidal agent, Antioxidant activity, Green synthesis, Coffea arabica, Zinc oxide nanoparticles

Main Article Content

Sulastri
Doctoral Program, Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University Makassar, 90245, Indonesia
Ahyar Ahmad
Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University Makassar,90245, Indonesia
Abdul Karim
Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University Makassar,90245, Indonesia
Isra Wahid
Department of Parasitology, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia
Wahyudin Rauf
Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University Makassar,90245, Indonesia
Harningsih Karim
Department of Pharmacy, School of Pharmacy YAMASI, Makassar, 90222, Indonesia
Andi M. Farid
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Pancasakti University, Makassar, 90223, Indonesia

Abstract

The ZnO-NPs have been successfully produced and characterized using Coffea arabica extract using the green method. The successful formation of nanoparticles was indicated by a color change brown. Phytochemical characterization revealed that Coffea arabica extract is rich in bioactive compounds, including alkaloids, flavonoids, and phenolics, which have significant antioxidant potential. The results of the antioxidant activity tests showed that increasing concentrations of Coffea arabica extract correlated with enhanced free radical scavenging activity, with a relatively low IC50 value, indicating this coffee extract can serve as an effective source of antioxidants. Histological assessment revealed damage to cell structures due to exposure to ZnO-NPs, including membrane dilation, epithelial layer damage, and signs of apoptosis and necrosis. The ZnO-NPs synthesized result from Coffea arabica extract showed possess larvicidal potential and beneficial antioxidant properties, however attention is needed regarding potential toxicity at certain concentrations.

Downloads

Download data is not yet available.

Article Details

How to Cite
Sulastri, Ahmad, A., Karim, A., Wahid, I., Rauf, W., Karim, H., & Farid, A. M. (2025). Green Synthesized Zinc Oxide Nanoparticles from Coffea arabica: Bioprospecting and Functional Potential as an Antioxidant and Larvicidal Agent against Aedes aegypti. Tropical Journal of Natural Product Research (TJNPR), 9(1), 90-96. https://doi.org/10.26538/tjnpr/v9i1.13
Issue
Vol. 9 No. 1 (2025): Tropical Journal of Natural Product Research (TJNPR)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Sulastri, Doctoral Program, Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University Makassar, 90245, Indonesia

Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Pancasakti University, Makassar, 90223, Indonesia

Ahyar Ahmad, Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University Makassar,90245, Indonesia

Research and Development Centre for Biopolymers and Bioproducts, LPPM, Hasanuddin University, Makassar, 90245, Indonesia

How to Cite

Sulastri, Ahmad, A., Karim, A., Wahid, I., Rauf, W., Karim, H., & Farid, A. M. (2025). Green Synthesized Zinc Oxide Nanoparticles from Coffea arabica: Bioprospecting and Functional Potential as an Antioxidant and Larvicidal Agent against Aedes aegypti. Tropical Journal of Natural Product Research (TJNPR), 9(1), 90-96. https://doi.org/10.26538/tjnpr/v9i1.13

References

1. Vijayaram S, Razafindralambo H, Sun YZ, Vasantharaj S, Ghafarifarsani H, Hoseinifar SH, Raeeszadeh M. Applications of green synthesized metal nanoparticles—a review. Biol. Trace Elem. Res. 2024; 202: 360–386.

2. Kandav G, Sharma T. Green synthesis: an eco-friendly approach for metallic nanoparticles synthesis. Part. Sci. Technol. 2024; 42(5): 874-894.

3. Aswathi VP, Meera S, Maria CGA, Nidhin M. Green synthesis of nanoparticles from biodegradable waste extracts and their applications: a critical review. Nanotechnol. Environ. Eng. 2023; 8:377–397.

4. Finore I, Feola A, Russo L, Cattaneo A, Di Donato P, Nicolaus B, Poli A, Romano I. Thermophilic bacteria and their thermozymes in composting processes: a review. Chem. Biol. Technol. Agric. 2023; 10(7): 1-22.

5. Maghraby YR, El-Shabasy RM, Ibrahim AH, Azzazy HME. Enzyme immobilization technologies and industrial applications. ACS Omega. 2023; 8: 5184-5196.

6. Elnahal ASM, El-Saadony MT, Saad AM, Desoky ESM, El-Tahan AM, Rady MM, AbuQamar SF, El-Tarabily KA. The use of microbial inoculants for biological control, plant growth promotion, and sustainable agriculture: A review. J. Plant Dis. Protect. 2022; 162: 759-792.

7. Sidhu AK, Verma N, Kaushal P. Role of biogenic capping agents in the synthesis of metallic nanoparticles and evaluation of their therapeutic potential. Front. Nanotechnol. 2022; 3: 1-17.

8. Devi L, Kushwaha P, Ansari TM, Kumar A, Rao A. Recent trends in biologically synthesized metal nanoparticles and their biomedical applications: a review. Biol. Trace Elem. Res. 2024: 202: 3383-3399.

9. Naseem K, Aziz A, Tahir MH, Ameen A, Ahmad A, Ahmad K, Arif M, Hassan W, Najeeb J, Rao E. Biogenic synthesized nanocatalysts and their potential for the treatment of toxic pollutants: environmental remediation, a review. Int. J. Environ. Sci. Technol. 2024; 21: 2163-2194.

10. Bhattacharjee N, Som I, Saha R, Mondal S. A critical review on novel eco-friendly green approach to synthesize zinc oxide nanoparticles for photocatalytic degradation of water pollutants. Int. J. Environ. Anal. Chem. 2024; 104(3): 489-516.

11. Vijayaram S, Razafindralambo H, Sun YZ, Vasantharaj S, Ghafarifarsani H, Hoseinifar SH, Raeeszadeh M. Applications of green synthesized metal nanoparticles—a review. Biol. Trace Elem. Res. 2024; 202: 360-386.

12. Priya SS, Vasantha-Srinivasan P, Altemimi AB, Keerthana R, Radhakrishnan N, Senthil-Nathan S, Kalaivani K, Chandrasekar N, Karthi S, Ganesan R, Alkanan ZT, Pal T, Verma OP, Proćków J. Bioactive molecules derived from plants in managing dengue vector Aedes aegypti (Linn.). Molecules. 2023; 28:2386.

13. Maia LJ, de Oliveira CH, Silva AB, Souza PAA, Müller NFD, Cardoso JC, Ribeiro BM, Santos de Abreu FV, Campos FS. Arbovirus surveillance in mosquitoes: Historical methods, emerging technologies, and challenges ahead. Exp. Biol. Med. 2023; 248(22): 2072-2082.

14. Anoopkumar AN, Aneesh EM. A critical assessment of mosquito control and the influence of climate change on mosquito-borne disease epidemics. Environ. Dev. Sustain. 2022; 24:8900–8929.

15. Gabiane G, Yen PS, Failloux AB. Aedes mosquitoes in the emerging threat of urban yellow fever transmission. Rev. Med. Virol. 2022; 4(32): 1-11.

16. El-Samad LM, Bakr NR, Abouzid M, Shedid ES, Giesy JP, Khalifa SAM, El-Seedi HR, El Wakil A, Al Naggar Y. Nanoparticles—mediated entomotoxicology: lessons from biologica. Ecotoxicology. 2024; 33: 305-324.

17. Mapossa AB, da Silva Júnior AH, Mhike W, Sundararaj U, de Oliveira CRS. Electrospun polymeric nanofibers for malaria control: Advances in slow-release mosquito repellent technology. Macromol. Mater. Eng. 2024; 309(8): 1-28.

18. Majeed H, Iftikhar T, Abid R. Green synthesis of zinc nanoparticles with plant material and their potential application in bulk industrial production of mosquito-repellent antibacterial paint formulation. React. Chem. Eng. 2024; 9(3): 677-683.

19. Patil S, Rajkuberan C, Joseph J. Biogenic nanoparticles as a strong larvicidal agent in integrated pest management. Biogen. Nanomater. Health Environ. 2024; 1: 1-14.

20. Kudesia N, Banu AN. Larvicidal efficacy of green synthesized silver nanoparticles on Aedes aegypti and its impact on nontarget Daphnia magna. Indian J. Microbiol. 2024: 6: 1-13.

21. Nie D, Li J, Xie Q, Ai L, Zhu C, Wu Y, Gui Q, Zhang L, Tan W. Nanoparticles: A potential and effective method to control insect‐borne diseases. Bioinorg. Chem. Appl. 2023; 1: 1-23.

22. Narayanan L, Kamaraj C. An investigation into the larvicidal activity of biologically synthesized silver and copper oxide nanoparticles against mosquito larvae. Chem. Biodivers. 2024; 4(21): 74.

23. Pouthika K, Madhumitha G. A review on plant-derived nanomaterials: an effective and innovative insect-resistant strategy for alternate pesticide development. J. Environ. Sci. Technol. 2024; 21: 2239-2262.

24. Khaire P, Mane S, Narute T, Musmade N. Nano-pesticides: A dab hand at eliminating pests. Nanotechnol. Sustainable Agric. 2024; 1:1-20.

25. Mosa MA, Ibrahim DSS, El-Tabakh MAM, El-Abeid SE. Recent advances in using nanotechnology in the management of soilborne plant pathogens. Nanotechnol. Plant Health. 2024; 1: 1-24.

26. Barbhuiya RI, Tinoco NN, Ramalingam S, Elsayed A, Subramanian J, Routray W, Singh A. A review of nanoparticle synthesis and application in the suppression of diseases in fruits and vegetables. Crit. Rev. Biotechnol. Taylor & Francis. 2024;64(14): 4477-4499.

27. Patil D. Surface modification using nanostructures and nanocoating to combat the spread of bacteria and viruses: recent development and challenges. J. Micromanufacturing. 2024; 25165984241228087.

28. Fatima A, Zaheer T, Pal K, Abbas RZ, Akhtar T, Ali S, Mahmood MS. Zinc oxide nanoparticles: Significant role in poultry and novel toxicological mechanisms. Biol. Trace Elem. Res. 2024; 202: 268-290.

29. Verma N, Kaushal P, Sidhu AK. Harnessing biological synthesis: Zinc oxide nanoparticles for plant biotic stress management. Front. Chem. 2024; 12: 01-16.

30. Saxena P, Harish, Shah D, Rani K, Miglani R, Singh AK, Sangela V, Rajput VD, Minkina T, Mandzhieva S,

Sushkova S. A critical review on fate, behavior, and ecotoxicological impact of zinc oxide nanoparticles on algae. Environ. Sci. Pollut. Res. 2024; 31: 19105-19122.

31. Jha S, Rani R, Singh S. Biogenic zinc oxide nanoparticles and their biomedical applications: a review. J. Inorg. Organomet. Polym. Mater. 2023; 33(10): 1437-1452.

32. Asif N, Amir M, Fatma T. Recent advances in the synthesis, characterization, and biomedical applications of zinc oxide nanoparticles. Bioprocess Biosyst. Eng. 2023;46(1):45-67.

Saeed M, Marwani HM, Shahzad U, Asiri AM, Rahman MM. Recent advances, challenges, and future perspectives of ZnO nanostructure materials towards energy applications. Chem. Rec. 2024;24(1):23-45.

Chaudhary S, Shivalkar S, Sahoo AK. Biosynthesis of zinc oxide nanoparticles and major applications. Mycosynthesis of Nanomaterials. 2023;12(3):101-125.

Abbasi R, Shineh G, Mobaraki M, Doughty S, Tayebi L. Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review. J. Nanopart. Res. 2023;25(2):150-175.

Allah MA Albo Hay, Alshamsi HA. Green synthesis of ZnO NPs using Pontederia crassipes leaf extract: characterization, their adsorption behavior, and anti-cancer property. Biomass Convers. Biorefinery. 2024;14(1):20-39.

Velsankar K, Parvathy G, Mohandoss S, Sudhahar S. Effect of green synthesized ZnO nanoparticles using Paspalum scrobiculatum grains extract in biological applications. J. Electron Micosc. Tech. 2022;8(5):243-259.

Kakasi B, Varga FJ, Nagy ST. Nanomaterials on living organisms: reduction of toxicity toward sustainability. Sustainable Nanomaterials: Synthesis and Applications. 2024;17(3):299-319.

Zöngür A, Zeybekler SE. Evaluation of the effects of zinc oxide (ZnO NPs) nanoparticles synthesized by green synthesis on Caenorhabditis elegans. Biol. Futur. 2024;75(1):12-25.

Rohani R, Dzulkharnien NSF, Harun NH, Ilias IA. Green approaches, potentials, and applications of zinc oxide nanoparticles in surface coatings and films. Bioinorg. Chem. Appl. 2022;19(2):77-95.

Alam M. Photocatalytic activity of biogenic zinc oxide nanoparticles: in vitro antimicrobial, biocompatibility, and molecular docking studies. Nanotechnol. Rev. 2021;10(3):149-167.

Clarke B, Ghandi K. The interplay of growth mechanism and properties of ZnO nanostructures for different applications. Small. 2023;19(1):1234-1256.

Pal K, Chakroborty S, Nath N. Limitations of nanomaterials insights in green chemistry sustainable route: review on novel applications. Green Processing and Synth. 2022;11(4):178-199.

Medhi R, Marquez MD, Lee TR. Visible-light-active doped metal oxide nanoparticles: review of their synthesis, properties, and applications. ACS Appl. Nano Mater. 2020;3(10):10248–10282.

Abdelbasir SM, McCourt KM, Lee CM, Vanegas DC. Waste-derived nanoparticles: synthesis approaches, environmental applications, and sustainability considerations. Fornt. Chem. 2020; 31(8): 1-18.

Halder M, Jha S. Medicinal plants and bioactive phytochemical diversity: a fountainhead of potential drugs against human diseases. Medicinal Plants: Biodivers. Biotechnol. Conserv. 2023;978-9811999369:121-147.

Chassagne F, Butaud JF, Ho R, Conte E, Hnawia É, Raharivelomanana P. Traditional medical practices for children in five islands from the Society archipelago (French Polynesia). J. Ethnobiol. Ethnomed. 2023; 19(1): 1-56.

Karati D, Varghese R, Mahadik KR, Sharma R, Kumar D. Plant bioactives in the treatment of inflammation of skeletal muscles: a molecular perspective. Evid. Based Complement. Altern. Med. 2022; 2022(1):4295802.

Şimşek A, Çiçek B, Turan E. The effect of chlorogenic acid from green coffee as a natural antioxidant on the shelf life and composition of hazelnut paste. Eur. Food Res. Technol. 2023; 249(4):847–859.

Yeager SE, Batali ME, Guinard JX, Ristenpart WD. Acids in coffee: A review of sensory measurements and meta-analysis of chemical composition. Crit. Rev. Food Sci. Nutr. 2023; 63(8):1010-1036.

Singh RK, Dhama K, Khandia R, Munjal A, Karthik K, Tiwari R, Chakraborty S, Malik YS, Bueno-Marí R. Prevention and control strategies to counter Zika virus, a special focus on intervention approaches against vector mosquitoes—current updates. Fornt. Microbiol. 2018: 8(9): 1-22.

Kaitana Y, Kurnia N, Tulung M, Tuda JSB, Mamahit JME, Tallei TE. The potential pangi leaf extract for Aedes spp. mosquito control. J. Adv. Biotechnol Exp Ther. 2023;6(1): 133-139.

KA S, JN, K VR. Zinc oxide-based antibacterial and antiviral functional materials. Antibacterial and Antiviral Functional Materials. ACS Publications. 2024;2:135-156.

Muthulakshmi L, Sundarapandian V, Nagapriyadarshini D, Annaraj J, Mathew MT, Nellaiah H. Larvicidal and Antimicrobial Activities of Green-Synthesized Ag Nanoparticles. Antimicrob. Antivir. Mater. 2022: 19;23-46

Abdulwahab Y, Ahmad A, Wahid I, Taba P. Coffee arabica-derived copper nanoparticles: A potent larvicidal agent against Aedes aegypti mosquitoes. J. Adv. Biotechnol Exp Ther. 2024;7(2): 314-327.

Kharat SN, Mendhulkar VD. Synthesis, characterization, and studies on antioxidant activity of silver nanoparticles using Elephantopus scaber leaf extract. Mater. Sci. Eng. 2016;62:719–724.

Husain JH, Arumugam D, Nawabjohn MS, Kumaran S, Pandurangan AK. Green Synthesis of Silver Nanoparticles Using Centratherum anthelminticum Extract against Breast Cancer Cells. Asian Pac. J. Cancer Prev.: APJCP. 2024;25(8):2711.

Ijoma KI, Ajiwe VIE, Ndubuisi. Evidence-Based Preferential In Vitro Antisickling Mechanism of Three Native Nigerian Plants Used In The Management of Sickle Cell Disease. Malaysian J. Biochem. Mol. Biol. 2022; 3: 9-17.