Argan Oil Mitigates Oxidative Stress Induced by Chronic Alcohol Consumption in Rats: An In Vivo and In Silico Study with Computational ADMET and Molecular Docking Analysis
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Abstract
The escalating issue of excessive alcohol consumption poses a significant health concern, leading to various disruptions across multiple physiological pathways. Argan oil (AO), derived from the fruit of “Argania spinosa L”, an indigenous tree native to southwestern Morocco, is renowned for its diverse health and well-being advantages. In this study, we focused on the impact of alcohol on the oxidation-reduction system, a critical aspect frequently influenced by alcohol. To investigate the potential mitigating effects of argan oil on oxidative stress, we employed three distinct treatment groups of rats: (1) alcohol-treated (3 g/kg), (2) alcohol (3 g/kg) and argan oil-treated (10 ml/kg), and (3) water-treated (control). Following a one-month treatment period, the rats were sacrificed, and their organs were utilized for biochemical assays. Results revealed that argan oil treatment significantly mitigated oxidative stress markers, as evidenced by a reduction in malondialdehyde (MDA) levels and restoration of antioxidant enzyme activities, such as superoxide dismutase (SOD) and catalase (CAT), toward normal levels. At the molecular level, ADMET and docking analyses demonstrated favorable binding interactions between argan oil molecules and proteins implicated in oxidative stress pathways. These findings underscore the potential of argan oil to restore equilibrium in the oxidation-reduction system, offering neuroprotective and antioxidant effects. This study provides foundational insights into the protective effects of argan oil against alcohol-induced oxidative damage, highlighting its potential as a therapeutic agent.
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