The Potency of Adenostemma platyphyllum and A. madurense as Antioxidant and Anti-aging Agents
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Abstract
Adenostemma platyphyllum and Adenostemma madurense are medicinal plants belonging to the same genus as A. lavenia. These plants are characterized by similar bioactivity. Therefore, this research is aimed at determining the antioxidant and anti-aging properties of A. platyphyllum and A. madurense, as well as identifying the potentially bioactive compounds in these plants. The plant extracts were obtained by successive maceration in n-hexane, ethyl acetate, and methanol in order to increase polarity. The total phenolic, total flavonoid, and total terpenoid contents were quantified using standard methods. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline sulfonate (ABTS) radical scavenging assays. The anti-aging activity was assessed using Schizosaccharomyces pombe yeast cells. The results showed that the methanol extract of A. platyphyllum had the highest phenolic content of 120.06 mg gallic acid equivalent (GAE)/g extract, while the ethyl acetate extract had the highest flavonoid and terpenoid contents of 58.62 mg quercetin equivalent (QE)/g extract and 6.88 mg nerol equivalent (NE)/g extract, respectively. The methanol extracts of the plants showed the highest antioxidant activity of 38.42 mg ascorbic acid equivalent (AAE)/g extract with IC50 value of 91.83 μg/mL. In addition, the methanol extracts showed the highest yeast cell viability, indicating anti-aging potential. The total phenolic content was strongly correlated with the antioxidant activity, suggesting that these substances were essential in the radical scavenging properties of the extracts.
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