Brine Shrimp Cytotoxicity and Anti-Mitotic Activity of Aqueous Root-Bark extract of Securidaca longepedunculata (Polygalaceae) doi.org/10.26538/tjnpr/v2i3.1

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Published: Mar 1, 2018
Keywords:
Allium cepa,, Brine Shrimp,, Securidaca longepedunculata,, cytotoxic, cancer

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Ridwan A. Lawal
Department of Biochemistry, College of Medicine, University of Lagos, P.M.B 12003, Lagos, Nigeria
Sodiq K. Lawal
Department of Human Anatomy, St Francis University College of Health and Allied Sciences, Ifakara, Tanzania.
Omolola S. Odesanmi
Department of Biochemistry, College of Medicine, University of Lagos, P.M.B 12003, Lagos, Nigeria.
Olufemi Isiaq
Department of Computing, Southampton Solent University, United Kingdom
Ikeoluwapo O. Adefisan
Department of Biological Sciences, College of Basic & Applied Sciences, Mountain Top University, Ogun State, Nigeria.
Ibrahim A. Badmus
Department of Biochemistry, College of Medicine, University of Lagos, P.M.B 12003, Lagos, Nigeria.
Jimoh O. Yusuf
Department of Biochemistry, College of Medicine, University of Lagos, P.M.B 12003, Lagos, Nigeria.
Osaretin A.T. Ebuehi
Department of Biochemistry, College of Medicine, University of Lagos, P.M.B 12003, Lagos, Nigeria.

Abstract

Securidaca longepedunculata is a savannah shrub found growing in South-West Nigeria and some other areas in Africa. It has been reported to be used in the treatment of over a hundred ailments. Its root bark is included in several anti-cancer decoctions in Nigeria. Despite its reported use as an anti-cancer plant, there is a dearth of information on the anti-cancer potential of Securidaca
longepedunculata in Nigeria. The aim of this research is to determine the cytotoxic and antimitotic activity of aqueous root bark extract of Securidaca longepedunculata. Securidaca longepedunculata aqueous root-bark extract was prepared and used for the study. Cytotoxic activity of S. longepedunculata extract was determined by the brine shrimp toxicity assay and Allium cepa assay was used to assess the anti-mitotic activity. Brine shrimp toxicity assay showed an LC50 of 25.1 µg/mL and the Allium cepa assay revealed that 100 mg/mL extract caused a reduction in mitotic index (MI) which was comparable to that of the standard drug, methotrexate.
Securidaca longepedunculata has potential as a cytotoxic agent.

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A. Lawal, R., K. Lawal, S., S. Odesanmi, O., Isiaq, O., O. Adefisan, I., A. Badmus, I., O. Yusuf, J., & A.T. Ebuehi, O. (2018). Brine Shrimp Cytotoxicity and Anti-Mitotic Activity of Aqueous Root-Bark extract of Securidaca longepedunculata (Polygalaceae): doi.org/10.26538/tjnpr/v2i3.1 . Tropical Journal of Natural Product Research (TJNPR), 2(3), 109-113. https://tjnpr.org/index.php/home/article/view/509
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Vol. 2 No. 3 (2018): Tropical Journal of Natural Product Research
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

A. Lawal, R., K. Lawal, S., S. Odesanmi, O., Isiaq, O., O. Adefisan, I., A. Badmus, I., O. Yusuf, J., & A.T. Ebuehi, O. (2018). Brine Shrimp Cytotoxicity and Anti-Mitotic Activity of Aqueous Root-Bark extract of Securidaca longepedunculata (Polygalaceae): doi.org/10.26538/tjnpr/v2i3.1 . Tropical Journal of Natural Product Research (TJNPR), 2(3), 109-113. https://tjnpr.org/index.php/home/article/view/509

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