Formulation and Evaluation of Therapeutic Potential of Nanoemulsion of a Blend of Antimicrobial Oils doi.org/10.26538/tjnpr/v2i2.3

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Published: Feb 1, 2018
Keywords:
Nanoemulsion,, Formulation,, Antimicrobial oils,, Therapeutic potential,, Safety.

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Uduma E. Osonwa
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka 420281, Nigeria.
Simeon A. Okechukwu
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka 420281, Nigeria.
Chibueze P. Ihekwereme
Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka 420281, Nigeria.
Kingsley I. Chukwu
Department of Pharmaceutics, College of Pharmacy and Nutrition, University of Saskatchewan, Canada.
Stanley C. Eluu
Department of Biotechnology, Ebonyi State University, Abakaliki, Ebonyi State, Nigeria.
Ricardo B. Azevedo
Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasilia, Brazil.

Abstract

This work is aimed at producing an effective self-emulsifying nanoformulation of a combination of 9 antimicrobial edible plant oils against bacterial and fungal infections. The oils are olive, eucalyptus, dill, castor, peppermint, garlic, ginger, sunflower and lemongrass oils. Oil Formulation (OF) was produced by mixing a simple Oil Blend (OB) of the oils with polysorbate 80 at the ratio of 1:4. Particle size distribution, polydispersity index and zeta potential of OF and Nanoemulsion (NE)were determined. The antimicrobial properties of OB and OF were determined using selected bacteria and fungi. The most sensitive bacteria and fungi were then selected for in vivo assay of OB and OF using albino Wistar rats (n = 5). Oral treatment began 4 days post-infection and lasted 5 days. Ciprofloxacin and Ketoconazole were used as positive controls. Safety screenings (hematological, liver function tests (LFT’s) and histological) were then
conducted. The results showed that the optimal Oil/Polysorbate 80 ratio was 1:4. NE had mean particle size (151.12 ± 6.21) nm, polydispersity index (0.12) and zeta potential (-13.70 ± 4.40) mV, which after 90 days became (202.50 ± 4.11) nm, 0.09, and (35.00 ± 6.15) mV respectively. OF had mean particle size (158.00 ± 3.08) nm, polydispersity index (0.07) and zeta potential (-15.50 ± 3.01) mV, which after 90 days became (162.90 ± 6.01) nm, 0.08, and (-19.00 ± 0.00) mV respectively. Nanoemulsion formulation of the oil blend significantly (p<0.05) improved antimicrobial activity in infections of Staphylococcus aureus and Aspergillus niger and it was not
toxic to the rats.

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How to Cite
E. Osonwa, U., A. Okechukwu, S., P. Ihekwereme, C., I. Chukwu, K., C. Eluu, S., & B. Azevedo, R. (2018). Formulation and Evaluation of Therapeutic Potential of Nanoemulsion of a Blend of Antimicrobial Oils: doi.org/10.26538/tjnpr/v2i2.3 . Tropical Journal of Natural Product Research (TJNPR), 2(2), 67-73. https://tjnpr.org/index.php/home/article/view/507
Issue
Vol. 2 No. 2 (2018): Tropical Journal of Natural Product Research
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

E. Osonwa, U., A. Okechukwu, S., P. Ihekwereme, C., I. Chukwu, K., C. Eluu, S., & B. Azevedo, R. (2018). Formulation and Evaluation of Therapeutic Potential of Nanoemulsion of a Blend of Antimicrobial Oils: doi.org/10.26538/tjnpr/v2i2.3 . Tropical Journal of Natural Product Research (TJNPR), 2(2), 67-73. https://tjnpr.org/index.php/home/article/view/507

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