Polymorphism of Catalase Gene and Serum Antioxidant Level in Iraqi Women Suffering from Spontaneous Abortion doi.org/10.26538/tjnpr/v5i7.4
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Abstract
Cupressus sempervirens (Cupressaceae) “cypress” is a specie native to the Mediterranean. Spontaneous abortion is a pregnancy failure that happens spontaneously before 20 weeks of gestation. Catalase (CAT) has been implicated to play a vital role in the protection of early pregnancy. The current study was aimed at investigating the polymorphism of catalase gene in relation to serum antioxidant level in Iraqi women suffering from spontaneous abortion. Fifty women with spontaneous abortion (study group) and fifty stable pregnant women (control group) were recruited for this study. Blood sample was collected from each subject for analyses. Vitamin D was measured by AFIAS-6 Compact Desktop Immuno-analyzer, while catalase activity was evaluated from serum by Colorimetric analysis. Also, the amount of Malondialdehyde (MDA) was determined. DNA was isolated from the blood samples and used for detecting polymorphism by RT-PCR. The results revealed that vitamin D and catalase activity were highly significant (p<0.0001) in the control than patient group. Conversely, the level of MDA was significantly (p<0.0001) higher in the patient group than control. The polymorphism assay indicated that CC genotype showed a significantly (p = 0.003) higher frequency in the patients (38%) than control (10%), while the TT genotype was higher in the control than in the patients group. Also, the CT genotype showed higher frequency in the control than patients group, but the difference was insignificant.
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