Characterization of Acid Treated Porcine Mucin and its Effects on Bioadhesion
doi.org/10.26538/tjnpr/v2i2.4
Keywords:
Mucin,, Acid treatment,, Modified,, Powder,, Analyses,, BioadhesionAbstract
The study aimed at determining the effect of acid modification on the physicochemical and bioadhesive properties of porcine mucin powder. Mucin powder was treated with solutions of acetic and hydrochloric acids at high and low concentrations and neutralized with equimolar volumes of pyridine and sodium hydroxide solutions, respectively after 24 h and dried. The treated powders were characterized using ultraviolet (UV) scans, solubility tests, pH, melting point, particle size analysis, Differential scanning calorimetry (DSC), Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), powder X-ray diffractometry
(PXRD) and bioadhesion measurements. Modified powder UV scans showed a noticeable shift in their peak of absorbances, generally
improved solubility, pH range of 4.20 - 4.90, melting point ranging from 105°C to 125°C and mean particle size range from 36.36 ± 6.65 to 114.25 ± 5.65 μm. Unmodified mucin DSC showed a sharp endothermic transition while those of the modified products were sharp and broad. The melting temperatures corresponded to the endothermic transitions (Tm), ranging from 105°C to 140°C. FTIR showed characteristic change in spectra pattern resulting in broadening of the OH band. PXRD showed distinct and more porous particles with the modified products. Their bioadhesion values ranged from 0.155 to 0.054 Nm-1 with the HCl modified mucin powders showing the highest bioadhesion. Acid modification of native porcine mucin powders resulted in the formation of modified products with superior bioadhesive properties over the native porcine mucin powders. These modified products have a potential application in bioadhesive drug delivery systems.
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