Effects of Hydroethanolic Leaf Extract of Ipomoea asarifolia (Convolvulaceae) in Doxorubicin and Isoproterenol-Induced Toxicity in Rats
doi.org/10.26538/tjnpr/v2i2.2
Keywords:
Ipomoea asarifolia,, Convolvulaceae,, Doxorubicin,, Isoproterenol,, Biochemical parameters,, Antioxidant indices.Abstract
This study investigated the effects of the hydroethanolic leaf extract of Ipomoea asarifolia on doxorubicin and isoproterenol-induced toxicities in rats. Rats were randomly assigned into groups- Group 1: distilled water (10 mL/kg orally), Group 2: toxicant, Groups 3-5: I. asarifolia (100, 200 and 400 mg/kg orally, respectively) and toxicant, and Group 6: gallic acid (15 mg/kg orally) and toxicant. Treatment lasted for 21 days in the doxorubicin (DOX) model and DOX (5mg/kg intraperitoneally) was administered on days 7, 14 and 21. Treatment was carried out for 30 days in the isoproterenol (ISO) model and ISO (85 mg/kg subcutaneously) was administered on
days 28-30. A day after the treatment, blood samples were collected for biochemical analysis and animals were sacrificed. Vital organs were harvested for in-vivo antioxidants assay. In the DOX model, I. asarifolia (100-400 mg/kg) produced significant (p < 0.01) reduction in levels of lactate dehydrogenase (LDH) and triglycerides (TG) relative to the DOX group. The extract reversed the
diminution in heart reduced glutathione (GSH) level caused by DOX. ISO significantly increased (p < 0.001) levels of LDH and TG, but these effects were reversed by I. asarifolia (100 and 400mg/kg). The extract (400 mg/kg) significantly reversed (p < 0.01) the increase in kidney MDA level elicited by ISO. I. asarifolia (200 and 400 mg/kg) significantly (p < 0.001) reversed the increase in liver MDA and reduction in CAT levels elicited by ISO. These findings suggest that the extract possesses cardioprotective effects against doxorubicin and isoproterenol-induced toxicities.
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