Antioxidative and Free Radical Scavenging Properties of Ethyl Acetate Fractions of Persea americana Seed and Bryophyllum pinnatum Leaf doi.org/10.26538/tjnpr/v5i8.26
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Abstract
Persea americana and Bryophyllum pinnatum are important plants with age-long application in traditional medicine for the management of varieties of ailments. The seed of P. americana is a byproduct and the leaf of B. pinnatum constituting weed in some locality could be harnessed for marketable antioxidant potentials. The present study aims to assess the antioxidative and free radical scavenging potential of ethyl acetate fractions of Persea americana seed and Bryophyllum pinnatum leaf. This was determined using the effect of the plant fractions on hydroxyl, nitric oxide, and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Total antioxidant capacity (TAC), reducing power assay and inhibition of H2O2-induced lipid peroxidation in rat stomach homogenates were assessed using standard methods. The extracts exhibited stronger DPPH radicals scavenging effect than tannic acid with threshold inhibitory concentration (IC50) values of 53.10 ± 2.66, 65.59 ± 3.28, and 2039.49 ± 163.16 µg/mL for P. americana, B. pinnatum, and tannic acid respectively. The ethyl acetate fractions exhibited strong hydroxyl and nitric oxide radicals scavenging ability in vitro, with IC50 values of 370.37 ± 14.81, 107.45 ± 5.37, 623.96 ± 31.20 µg/mL and 377.26 ± 18.86, 691.48 ± 48.40,105.86 ± 6.35 µg/mL for P. americana, B. pinnatum, and catechin respectively. Furthermore, the B. pinnatum leaf fraction was found to be highly effective in the inhibition of H2O2-induced lipid peroxidation in rat stomach homogenates. The present study showed that the ethyl acetate fractions of P. americana seed and B. pinnatum leaf possess potent antioxidant properties evidenced in their radical scavenging ability.
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