LC-MS Based Secondary Metabolites Profile of Elaeocarpus grandiflorus J.E. Smith. Cell Suspension Culture Using Picloram and 2,4-Dichlorophenoxyacetic Acid doi.org/10.26538/tjnpr/v5i8.13

Abstract

Elaeocarpus grandiflorus contains prominent bioactive compounds. The bioactive metabolites can be increased using the cell suspension culture technique by adding synthetic auxin, including picloram and 2,4-dichlorophenoxyacetic acid (2,4-D). Therefore, this study aimed to analyze the effect of picloram and 2,4-D on the secondary metabolite profile of E. grandiflorus cell suspension culture. Petioles of young leaves from E. grandiflorus were used as explants for callus induction, and then the callus was used for cell suspension culture. The cell culture was maintained on a woody plant medium (WPM) for 30 days supplemented with picloram (3.5 mg/L; 5.0 mg/L; and 7.5 mg/L), or 2,4-D (1.5 mg/L; 2.5 mg/L; and 3.5 mg/L). The 2.5 mg/L  2,4-D treatment with the highest dry weight was harvested every five days until the 30th day. Secondary metabolites in all treatments showed no significant difference (P = 0.949, F3.6 = 0.228), and the highest content of secondary metabolites was kaempferols which was up to 24.29 ± 0.77%, while the total average flavonoid content was up to 55.69 ± 0.96%. In addition, the secondary metabolites did not change significantly for 30 days (P = 0.974, F3.6 = 0.279). Most plant energy and hormones were used for cell division and growth instead of secondary metabolite biosynthesis during this period. This study showed that picloram and 2.4- D induction have no significantly different effect on the secondary metabolite profile in the E. grandiflorus cell suspension culture.