Fmrfamide Mitigated MDMA (3, 4- Methylenedioxymethamphetamine)/Tramadol-Induced Testicular Toxicity in Wistar Rat https://doi.org/10.26538/tjnpr/v8i7.34
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Abstract
MDMA (3,4-methylenedioxymethamphetamine) and tramadol are central nervous system stimulants known for inducing emotional communion and socializing effects. FMRFamide, an RFamide peptide isolated from mollusks, plays diverse roles in physiological processes. This study aimed to evaluate FMRFamide's impact on testicular toxicity induced by MDMA/tramadol in Wistar rats. Thirty male Wistar rats were divided into six groups (n=5). Group A served as the control, Group B received 2 mg/kg FMRFamide for two consecutive days. Groups C, D, and E received MDMA/tramadol (20 mg/kg) for ten days, while Group F received the same dosage along with FMRFamide. Sperm analysis, biochemical, and histological assessments were conducted. The results revealed significant body weight loss, decreased sperm count and motility, increased abnormal sperm formation, and reduced serum LH, FSH, and Testosterone levels. Inducible Nitric Oxide Synthase (iNOS) expression was intensified in Groups A, B, D, and F. Notably, FMRFamide administration attenuated the adverse effects of MDMA/tramadol-induced testicular toxicity, suggesting a potential therapeutic role in safeguarding male reproductive health. These findings highlight the importance of further exploring FMRFamide's protective mechanisms and its potential application in mitigating drug-induced reproductive toxicity.
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