Optimization of Total Flavonoid Content from Cardamom Fruits Using a Simplex-Centroid Design, Along with the Evaluation of the Antioxidant Properties doi.org/10.26538/tjnpr/v5i8.10

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Published: Aug 1, 2021
Keywords:
Amomum compactum Sol. Ex Maton, DPPH, Flavonoid, FRAP, Mixture designs, Solvent extraction

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Waras Nurcholis
Department of Biochemistry, Faculty of Mathematic and Natural Science, IPB University, Bogor, Indonesia
Khusnul Ma’rifah
Department of Biochemistry, Faculty of Mathematic and Natural Science, IPB University, Bogor, Indonesia
Made I. Artika
Department of Biochemistry, Faculty of Mathematic and Natural Science, IPB University, Bogor, Indonesia
Syarifah I. Aisyah
Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia
Bambang P. Priosoeryanto
ropical Biopharmaca Research Center, IPB University, Bogor, Indonesia

Abstract

The fruits of Cardamom (Amomum compactum Sol. Ex Maton; Zingiberaceae), commonly known as Queen species, which contained flavonoid compounds, have a variety of pharmacological properties and are used in traditional medicine. Therefore, this study aimed to employ solvent macerations to optimize the total flavonoid extraction from Cardamom fruits, as well as evaluate their antioxidant activity. The fruits were collected from a local farmer in Bogor, Indonesia, in August 2019. Then, different extraction solvents, namely water, acetone, methanol, and ethanol, alongside their binary, ternary, and quaternary combinations, were investigated using a simplex-centroid design. The optimized extracts were tested for their flavonoid content using an aluminium chloride reagent and their antioxidant activity compared by utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Water extracts showed the maximum flavonoid content of 88.01 mg/g DW with the highest reducing antioxidant activity at 1110.95 mol TE/g DW by FRAP assay. Meanwhile, the radical scavenging activity by the DPPH method revealed the highest value for the water-ethanol binary mixture to be 153.17 mol TE/g DW. Consequently, the water and water-ethanol are the optimal solvents for extracting cardamom flavonoid compounds with antioxidant properties.

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How to Cite
Nurcholis, W., Ma’rifah, K., I. Artika, M., I. Aisyah, S., & P. Priosoeryanto, B. (2021). Optimization of Total Flavonoid Content from Cardamom Fruits Using a Simplex-Centroid Design, Along with the Evaluation of the Antioxidant Properties: doi.org/10.26538/tjnpr/v5i8.10. Tropical Journal of Natural Product Research (TJNPR), 5(8), 1382-1388. https://tjnpr.org/index.php/home/article/view/443
Issue
Vol. 5 No. 8 (2021): Tropical Journal of Natural Product Research
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Waras Nurcholis, Department of Biochemistry, Faculty of Mathematic and Natural Science, IPB University, Bogor, Indonesia

Tropical Biopharmaca Research Center, IPB University, Bogor, Indonesia

Bambang P. Priosoeryanto, ropical Biopharmaca Research Center, IPB University, Bogor, Indonesia

Division of Veterinary Pathology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia

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