Hematological and Histopathological Changes in the Spleen of Male Albino Rats (Rattus norvegicus) Treated with Meloxicam doi.org/10.26538/tjnpr/v5i8.8
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Abstract
Meloxicam is a non-steroidal anti-inflammatory drug, which works by lowering the levels of hormones that cause inflammation and pain in the body. However, its effect on human organs is yet to be investigated. Therefore, this study was aimed at examining the hematological and histopathological impacts of meloxicam drug on the spleen of male albino rats (Rattus norvegicus). Male albino rats, weighing 180-200 g and aged 6-7 weeks, were divided into two groups of ten rats each; experimental and control. The control group was administered with a sterile normal saline solution, while the experimental group received a meloxicam solution, both for seven weeks. At the end of the experiment, the animals were sacrificed and the tissues were subjected to hematological and histological investigations. Some hematological parameters were determined. The results revealed histological changes in the spleen tissues of meloxicam-treated experimental rats, including the emergence of cellular degeneration and necrosis in both the white and red pulp. The presence of hemosiderin in the red pulp and cellular degeneration in the marginal zone as well as hyperplasia in the germinal center in the white pulp were also observed. Experimental animals administered with meloxicam revealed no significant changes in the number of red blood cells or packed cell volume in the hematological parameters. However, the platelets, white blood cells, and hemoglobin were significantly higher in the experimental group. The findings of this study revealed that meloxicam induces histological changes in the spleen architecture, as well as aberrant abnormalities in the various hematological parameters examined.
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References
Mabmoud FY, Abou Elghait AT, Rateb A, Salah E. Comparative study on the effect of long-term administration of non- steroidal anti-inflammatory drugs Meloxicam and ketoprofen on the structure of the kidney and gastric mucosa in adult albino rats. Egy J Histol. 2010; 33 (4):722-734.
Andalib S, Naeini AM, Garjani A, Asl NA, Abdollahi A. A comparative study pertaining to deleterious effects of diclofenac sodium and meloxicam on kidney tissue in rats. EXCLI J. 2011; 10(1):149-154.
Al-Rekabi FMK, Abbas DA, Hadi, NR. Effect of subchronic Exposure to Meloxicam on some hematological biochemical and liver histopathological parameters in rats. Iraqi J Vet Sci. 2009; 23(2):249-254.
Weir M. Renal effects of nonselective NSAIDs and coxibs. Cleve Clin J Med. 2002; 69 (1):53-58.
Modi CM, Mody SK, Patel HB, Dudhatra GB, Kumar A. Avale M. Toxic pathological over view of analgesic and anti-inflammatory drug in animals. J Appl Pharm Sci. 2012; 2(1):149-157.
Ricciotti E, Garret A, Gerald F. Prostaglandin and inflammation. Arterioscler Thromb Vasc Biol 2011; 31(5): 986-1000.
Ahmed MY, Abdul-Fattah JH, Aziz FM. The protective role vitamin C against the Hepatotoxic and nephrotoxic effect of Meloxicam in male mice. Int J Pharm Bio Sci. 2015; 10(5):69-73.
Gassel AD, Tobias KM, Egger CM, Rohrbach BW. Comparison of oral and subcutaneous administration of buprenorphine and meloxicam for preemptive analgesia in cats undergoing ovariohysterectomy. J Am Vet Med Assoc. 2005; 227(12):1937-1944.
Kirchgessner MS. Meloxicam. J Exotic Pet Med. 2006; 15(4): 281-283.
Bashandy SA and Alwasel SH. Carbon TetrachlorideInduced Hepatotoxicity and nephrotoxicity in rats: protective Role of vitamin C. J. Pharmacol Toxicol. 2011; 6(3):283-229.
Bronte V and Pittet MJ. The spleen in local and systemic regulation of immunity. Immunity. 2013; 39(5):806-818.
Lewis SM, williams A, Eisenbarth SC. Structure-function of the immune system in the spleen. Sci Immunol. 2019; 4 (33):1-12.
Calabro S, Liu D, Gallman A, Nascimento MS, Yu Z, Zhang TT, Chen P, Zhang B, Xu L, Gowthaman U, Krishnaswamy JK, Haberman AM, Williams A, Eisenbarth SC. Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity. Cell Rep. 2016; 16(9):2472-2485.
Germolec DR, Kashon M, Nyska A, Kuper CF, Portier C, Kommineni C, Johnson KA, Luster MI. The accuracy of extended histopathology to detect immunotoxic chemicals. Toxicol Sci. 2004; 82(2):504-514.
Baraaj AH. Histological, biochemical and DNA changes in the liver of male albino rats treated with silver nanoparticles. Nano Biomed Eng. 2021; 13(1):20-26.
Carpenter JW, Pollock CG, Koch DE, Hunter RP. Single and multiple-dose pharmacokinetics of meloxicam after oral administration to the rabbit (Oryctolagus cuniculus). J Zoo Wildl Med. 2009; 40(4): 601-606.
Bancroft JD and Steven AS. Theory and practice of histological techniques. (2nd Ed.) Churchill living stone, Edinburgh. London. 2012; pp233-250.
Casacó A, Beausoleil I, Gonzalez B, Luaces P, León A, Arteaga ME, Prado P, Rodríguez V, Pérez A, Guevara G, Bada AM, Ledón N, Fuentes D, González C, Hernandez O, Orphee R, Blanco D, García-Osuma M, Ballester-LabradaA. Hematological, biochemical, respiratory, cardiovascular
and electroneurophysiological parameters in African green monkeys (Cercopithecus aethiops sabaeus). Its use in nonclinical toxicological studies. J Med Primatol. 2010; 39(3): 177-186.
Illukkumbura A. Introduction to categorical data analysis (fifth book) easy statistics series. Independently published, may12, 2021, pp29-31.
Bessone F. Non-steroidal anti-inflammatory drugs: What is the actual risk of liver damage? World J Gastroenterol. 2010; 16(45): 5651-6561.
Weir M. Renal effects of non-selective NSAIDs and coxibs. Cleve Clin J Medicine. 2002; 69(1):53-58.
Burukoglu D, Baycu C, Taplamacioglu F, Sahin E, Bektur E. Effects of nonsteroidal anti-inflammatory meloxicam on stomach, kidney, and liver of rats. Toxicol Ind Health. 2016; 32(6):980-986.
Jadav R, Patel B, Joshi D, Patel G, Raval S. Toxipathological studies of experimentally inducedMeloxicam toxicity in Wister rats (Rattus norvegius). Wayamba J Anim Sci. 2014; 16 (1):870-875.
Elmore SA. Enhanced Histopathology of spleen. Toxicol Pathol. 2006; 34:648-655.
Suttie AW. Histopathological of spleen. Toxicol Pathol. 2006; 34:466- 480.
Pachathundikandi SK and Varghese ET. Blood zinc proto porphyrin, serum total protein and total cholesterol levels in automobile workshop workers in relation to lead toxicity: our Experience. Indian J Clin Biochem. 2006; 21(2):114-117.
El-Sharaky AS, Newairy AA, Elguindy NM, Elwafa AA. Spermatotoxicity, biochemical changes and histological alteration induced by gossypol in testicular and hepatic tissues of male rats. Food Chem Toxicol. 2010; 48(12): 3354-3361.
Amin HM, El-Feki, MA, Abdalla AA, Youssef MA. Hematological and Biochemical Effect of Meloxicam in male Albino Rats. Curr Sci Int. 2017; 6(1): 23-33.
Tavakoli M.M, Nazifi S, Mosleh N, Hosseinian SA, Nakhaee P. Comparative Effects of Dexamethasone and Meloxicam on Magnitude of the Acute Inflammatory Response Induced by Escherichia coli Lipopolysaccharide in Broiler Chickens. J Inflamm Res. 2020; 13: 487-495.
Ibrahim KE, AL-Mutary MG, Bakhiet AO and Khan HA. Histopathological of liver, kidney and spleen of Mice exposed to gold nanoparticles. Molecules. 2018; 23(8): 1848-1861.
Kaid F, Alabsi AM, Alafifi N, Saeed RA, Al-koshab MA, Ramanathan A and Ali AM. Histological, Biochemical and Hematological effects of Goniothalamin on selective internal organs of male Sprague- Dawley rats. J Toxicol. 2019; 1(1): 1-13.
Cataldi M, Viglliotti C, Mosca T, Cammarota MR, Capon D. Emerging role of the spleen in the pharmacokinetics of monoclonal antibodies, nanoparticles and Exosomes. Int J Mol Sci. 2017; 18(6):12-49.
Orinya OA, Adenkola AV, Ogbe RJ. Hematological and Biochemical studies on the effect of diclofenac sodium on Wister Rattus norvegicus. Int J Biol Chem Sci. 2016; 10 (5): 2231-2242.
Shehata MM, Saad Eldien HM, Meligy FY, Bahaidarh SY. The Possible Protective Role of Barley Seeds on the Spleen after Administration of Glucocorticoids in Adult Albino Rats: A Histological and Immunohistochemical Study. J Microsc Ultrastruct. 2019; 7(4):171-180.
Yan SX, Deng XM, Wang QT, Sun XJ, Wei W. Prednisone treatment inhibits the differentiation of B lymphocytes into plasma cells in MRL/MpSlac-lpr mice. Acta Pharmacol Sin. 2015; 36(11):1367-1376.