Inhibitory Effects of Radish Peeled Root Extracts against Some Pathogenic Bacteria doi.org/10.26538/tjnpr/v5i8.5
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Abstract
Several studies have shown the ability of extracts from different plant parts to suppress the growth of pathogenic bacteria, including drug-resistant strains. This study was aimed to investigate the antibacterial effectiveness of radish (Raphanus sativus) root peel extracts against selected pathogenic bacteria. Different clinical samples were collected in period between December 2019 - June 2020 in Tikrit city of Iraq. The Identification of isolates was conducted using microscopic, macroscopic cultural characteristics and biochemical tests. Ethanol and methanol extracts were prepared from radish root peels. The Kirby-Buer well diffusion assay was employed to test the antibacterial activities of the extracts, selected antibiotics, and interaction between both agents. Virulence factors were detected before and after exposure of test isolates to the extracts. The results indicated that Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were the predominant isolates. The methanol extract showed more inhibitory effect than the ethanol extract against the test pathogenic bacteria. The concentration of 100 mg/mL of the methanol extract had higher inhibitory activity compared to other concentrations. A synergistic inhibitory effect between the extracts with test antibiotics was obtained against a wide variety of bacteria, thereby expanding the target spectrum. In addition, it was observed that biofilm production was the factor most affected, suggesting the possibility of the extracts reducing the virulence of the test bacteria. It can be concluded that the combination of radish peeled root methanol extract with ethanol extract or antibiotics inhibited bacterial growth synergistically.
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Törün B, Çoban EP, Biyik H, Barişik E. Antimicrobial activity of Echinophora tenuifolia L. and Raphanus sativus L. extracts. Indian J Pharm Edu Res. 2017;51(3):373-376.
Elgayyar M, Draughon FA, Golden DA, Mount JR, Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. J Food Protec. 2001; 64(7):1019-1024.
Abdallah NA, Abd El –Rahman RZ, Amer AK, IsmaeelLIM. Antibacterial activity of some plant extracts on the treatment of surgical wounds infection. Egypt J Exp
Biol. 2013; 9(1):115-124.
Rani I, Akhund S. Abro H. Antimicrobial potential of seed extract of Raphanus sativus. Pak J Bot. 2008; 40(4): 1793-1798.
Janjua S, Shahid M, Abbas F. Phytochemical analysis and in vitro antibacterial activity of root peel extract of Raphanus sativus L. var niger. J Adv Med Plant Res. 2013; 1(1):1-7.
Anggraini F, Satari MH, Mariam MS. Bacterial inhibition test of methanol extracts of strawberry (Fragraia x ananassa Duchesne), lime (Citrus aurantifolia), and radish (Raphanus sativus L.), towards Streptococcus sanguis ATCC 10556. Padjadjaran J Dent.
; 30(2): 98-102.
Mahmoodn YSH. Green synthesis of copper oxide nanoparticles using aloe vera and studying antibacterial effect against chronic suppurative Otitis Media, Tikrit University, College of Science, Diploma Thesis. 2019; 38-40.
McFarland J. The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc. 1907; 49(14):1176-1178.
Lim HW, Song KY, Chon JW, Jeong D, Seo KH. Antimicrobial action in Raphanus raphanistrum subsp sativus radish extracts against foodborne bacteria present in various milk Products. J Milk Sci Biotechnol. 2019; 37(3):187-190.
Ogueke CC, Ogbulie JN, Anyanwu BN. The effects of ethanol and boiling water extracts of root barks and leaves of Uvaria Chamae on some hospital isolates. J Am Sci. 2007; 3(3):68-73.
Al-Hadhrami, Rehab Mohammed Said, Raqiya Mohsin Said Al Muniri, and Mohammad Amzad Hossain. Evaluation of antimicrobial and cytotoxic activities of polar solvent extracts from leaves of Ammi majus used by the omanis. Pacific Sci Review A: Nat Sci Engineer. 2016; 18(1):62-65.
Clinical Laboratory Standards Institute. Performance Standard for Antimicrobial Disk Susceptibility Tests. 2018; 31(1):1-94
Mahmood YSH, Abed SM, Alwan AM. Isolation, identification of bacterial species causing chronic suppurative otitis media and detection of some of their virulence factors. Tikrit J Pure Sci. 2019; 24(7):45-51.
Oliveira A & Maria de Lourdes RS. Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci. J BMC Res Notes. 2010; 3(1):1-8.
Birri DJ, Brede DA, Tessema GT, Nes IF. Bacteriocin production, antibiotic susceptibility and prevalence of haemolytic and gelatinase activity in faecal lactic acid bacteria isolated from healthy Ethiopian infants. J Microb Ecol. 2013; 65(2):504-516.
Mahmood WS, Alrifae SB, Abed SM. The efficiency of oral ketoconazole in reduction of lipase enzyme activity of cutibacterium acnes in acne vulgaris patients, Int J Pharm Res. 2020;12(3):1283-1286.
Malarkodi C, Chitra K, Rajeshkumar S, Gnanajobitha G, Paulkumar K, Vanaja M, Annadurai G. Novel ecofriendly synthesis of titanium oxid nanoparticles by using Planomicrobium sp. and its antimicrobial evaluation. Der Pharmacia Sinica. 2013; 4(3):59-66.
Khazaal AS, Ismeel NN, Hussein AK. Statistical study to check the conformity of aggregate in Kirkuk City to requirement of Iraqi specification. Tikrit J Eng Sci. 2015; 2(22):1-18.
Ahmad F, Hasan I, Chishti DK and Ahmad H. Antibacterial activity of Raphanus sativus Linn. seed extract. Global J Med Res. 2012; 12(11):25-34.
Reda FM, El-Zawahry YA and Omar AR, Synergistic effect of combined antibiotic and methanol extract of Eucalyptus camaldulensis leaf against Staphylococcus aureus and Pseudomonas aeruginosa. Int J Appl SciBiotechnol. 2017; 5(4):486-497.
Nagappan R. Evaluation of aqueous and ethanol extract of bioactive medicinal plant, Cassia didymobotrya(Fresenius) Irwin and Barneby against immature stages of filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae). Asian Pac J Trop Biomed. 2012; 2(9):707-711.
Aghemwenhio IS, Timilehin AA, Alpheus GA. Susceptibility of beta-haemolytic Escherichia coli to commonly used antibiotics in selected hospitals in Delta State, Southern Nigeria. Arc Clin Microbiol. 2017; 8(2): 36.
Al-ani MSH. Study the synergistic effect of some natural substances with the effectiveness of antibiotics on ciprofloxacin-resistant bacteria isolated from urinary
tract infection, Higher Diploma Thesis / Pathological Analysis. College of Science, University of Tikrit. 2018. 43 p.
Fatma S, Arrish FS, Baraka MA, Alzowi SA. Study of the synergistic effect of the alcoholic extract of leaves and flowers of Calotropis procera plant with the antibiotics on two types of bacteria: Salmonella typhimiriim and Streptococcis faecalis. J Pure Appl Sci. 2018; 17(2):82-87.
Ennacerie FZ, Filali FFR, Moukrad N. Antibacterial synergistic effect of extracts of the organs of Capparis spinosa in combination with antibiotics. Int J Adv Res. 2017; 5(9):1238-1247.
Anggraini F, Satari MH, Mariam MS. Bacterial inhibition test of methanol extracts of strawberry (Fragraia x ananassa Duchesne), lime (Citrus aurantifolia), and radish (Raphanus sativus L.), towards Streptococcus sanguis ATCC 10556. Padjadjaran J Dent.
; 30(2):98-102.
Muthusamy B and Shanmugam G. Analysis of flavonoid content, antioxidant, antimicrobial and antibiofilm activity of in vitro hairy root extract of radish (Raphanus
sativus L.). J Plant Cell Tiss Organ Cult. 2020; 140: 619-633.
Saleh TH, Hashim ST, Malik SN, AL-Rubaii BAL. Down-Regulation of flil gene expression by Ag nanoparticles andTiO2 nanoparticles in pragmatic clinical isolates of Proteus mirabilis and Proteus vulgaris from urinary tract infection. Nano Biomed Eng.2019; 11(4):321-332.
Ali MAM and Al-Rubaii BAL. Study of the Effects of Audible Sounds and Magnetic Fields on Staphylococcus aureus Methicillin Resistance and mecA Gene Expression. Trop J Nat Prod Res. 2021; 5(5):825-830.