Brine Shrimps Toxicity, Bactericidal and Antifungal activity of Monodora myristica (Gaertn) Essential Oils doi.org/10.26538/tjnpr/v2i1.8
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Abstract
Essential oils from the seeds and stem bark of Monodora myristica were obtained through hydro-distillation using Clevenger-type apparatus. The toxicities of the volatile oils were assayed using the brine shrimps’ toxicity assay and the LC50 (median lethal concentration) was calculated using Finney’s probit analysis. The antimicrobial assay was carried out using the agar diffusion method. Ten microbes consisting of five bacteria and five fungi were used in this study. The bacteria were three species of Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Salmonella typhi) and two Gram-negative bacteria (Escherichia coli, and Pseudomonas aeruginosa) and the fungi were Candida albicans, Candida krusei, Candida glabrates, Aspergillus niger and Penicillum nostatum. Monodora myristica seeds and stem-bark displayed an equal level of toxicity (LC50 = 0.1 µg/mL) in brine shrimps lethality assay. In comparison to standard antibiotics (Gentamicin) and antifungal agent (Tioconazole), the activity of Monodora myristica essential oils for growth inhibition of the test microorganisms was low with the zone of inhibition of 18.0 ± 2.0 mm recorded against Bacillus subtilis, the most susceptible organism to the essential oil.
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Ananiel K, Hudson JB Amason JT, Gbeassor, M. Investigation of medicinal plants of Togo for antiviral activity. Pharm Biol. 2000; 38:40-45.
Werka JS, Boehmeb AK, Setzera WN. Biological Activities of Essential Oils from Montverde, Costa Rica. Nat Prod Commun. 2007; 2:12.
Prakash P and Gupta N. Therapeutic uses of Ocimum sanctum LINN (tulsi) with a note on eugenol and its pharmacological actions: a short review. Ind J Phys Pharm. 2005; 49(2):125-131.
Dorman HJD, Deans SG, Noble RC, Surai P. Evaluation in vitro of plant essential oils as natural antioxidants. J Ess Oil Res. 1995; 7:645-651
.
Dobre AA, Gagiu V, Petru N. Antimicrobial activity of essential oils against food-borne bacteria evaluated by two preliminary methods. Rom Biotech Lett. 2011; 16(6):1-6.
Kubo A and Kubo I. Antimicrobial agents from Tanacetum balsamita. J Nat prod. 1995; 58(10):1565-1569.
Fournier G, Leboeuf M, Cave A. Annonaceae essential oils: a review. J Ess Oil Res. 1999; 11:131.
Okafor JC. Development of forest tree crops for food supplies in Nigeria. Forest Ecol Manage. 1987; 1:235.
Ekeanyanwu CR, Ogu LG, Nwachukwu UP. Biochemical characteristics of the African nutmeg, Monodora myristica. Agric J. 2010; 5(5):303
Uwakwe AA and Nwaoguikpe RNJ. Invitro and antisickling effect of Xylopia aethiopica and Monodora myristica. Med Plant Res. 2008; 2(6):110.
Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Illinois, USA. 1995; 5-115 p.
Krishnaraju AV, Rao-Tayi VN, Vanisree M, Tsay HS, Subbaraju GV. Assessment of bioactivity of Indian medicinal plant using brine shrimp (Artemia salina) Lethality Assay. Int J Appl Sci Eng. 2005; 3(2):125-134.
Washington JE and Sutter VL. Dilution Susceptibility Tests, Agar and microbroth Dilution procedure. Manual of Clinical Microbiology, 3rd Edition.Washington DC: Am Soc Micr.1980; 453 p.
Owokotomo IA and Ekundayo O. Comparative study of the essential oils of Monodora myristica from Nigeria. Eur Chem Bull. 2012; 1(6):263-265.
Carson CF, Mee BJ, Riley TV. Mechanism of action of Melaleca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage and salt tolerance assays and electron microscopy. Ant Agents Chemother. 2012; 46: 1914–1920