Antioxidant and α-Glucosidase Inhibitory Activities of Phenolic Compounds Isolated from the Aerial Parts of Ampelocissus africanus doi.org/10.26538/tjnpr/v5i9.25
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Abstract
Ampelocissus africanus is an important medicinal plant with diverse ethnomedicinal uses. The purpose of this study was to investigate the antioxidant and α-glucosidase inhibitory activities of phenolic compounds isolated from the aerial parts of Ampelocissus africanus.Two known compounds were isolated using a combination ofcolumn and thin layer chromatography. The structures were elucidated usingUV and NMR spectroscopy. The antioxidant activity of the compounds was evaluated using 2,21-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, while the antidiabetic potential was investigated using the α-glucosidase inhibition assay. Ascorbic acid and acarbose were used as standard and data obtained were expressed as mean ± SEM.The IC50 of the two compounds were calculated using a suitable regression analysis and graph pad prism software 6.0 using t-test.Compounds 1 and 2 were isolated as a yellow solids and identified as methyl gallate and quercetin, respectively. Result of the antioxidant assay using ABTS revealed that compound 1 gave a percentage inhibition of 98.22±0.79, while compound 2 gave 99.04±0.39% inhibition. The DPPH assay revealed inhibition at 59.38±3.23% and 63.45±2.56% for compounds I and 2, respectively. The values for DPPH assay were significantly different at p value <0.05 when compared to ascorbic acid. Compound 2 exhibited α-glucosidase inhibitory activity (IC50 4.02±0.37 μg/mL) though lower than acarbose (IC50 0.98± 0.57 μg/mL). Compound 1 however, showed weak α-glucosidase inhibitory activity with IC50>100µg/mL. These results might justify the ethnomedicinal uses of this plant in ameliorating metabolic disorder such as diabetes.
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