Phytochemical Investigation, Comparison and Characterization Study of Malaysian Stingless Bee Honey versus Jordanian Honey by LC-MS/MS doi.org/10.26538/tjnpr/v5i9.12
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Abstract
Phytochemical studies have revealed more than 200 distinct polyphenolic compounds present in honey alone. Stingless bee honey (Trigona honey) is naturally found in tropical and subtropical regions. This study aims to interrogate the physical parameters, polyphenolic content, and antioxidant characteristics of Malaysian Trigona honey in comparison with Apis honey (Centaurea hyalolepis) and Citrus honey from Jordan. The three honey types were subjected to phytochemical and chromatographic analysis to explore the differences in honey composition related to bees type and geographical location. The total phenolic content ranged between 288.09 and 663.19 mg GAE/kg of honey in the three honey types. Trigona honey had the highest phenolic content (663.19 mgGAE/kg) followed by Centaurea hyalolepis honey (471.87 mgGAE/kg), both of which were higher than Citrus honey (288.09 mgGAE/kg). Trigona honey showed an IC50 of 61.042 ± 0.45 mg/mL, whereas, Centaurea hyalolepis honey and Citrus honey had IC50 of 120.29 ± 1.64 mg/mL and 129.51 ± 4.3 mg/mL, respectively. Statistical analysis has revealed a significant negative correlation between the IC50 value for the three honey samples and the concentration of polyphenols (p≤0.001). Chromatographic analysis using LC-MS/MS showed a 28 and 42-fold difference in the polyphenolic content in Trigona over C.hyalolepis and Citrus honey, respectively. In conclusion, the diversity in the polyphenols contents and the high amounts of phytochemical compounds found in Trigona honey confers the antioxidant activity and there is no unique compound responsible for such activity over C. hyalolepis and Citrus honey.
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Jibril FI, Hilmi ABM, Manivannan L. Isolation and characterization of polyphenols in natural honey for the treatment of human diseases. Bull Natl Res Cent. 2019;43(1):4-11
Aw Yong PY, Islam F, Harith HH, Israf DA, Tan JW, Tham CL. The Potential use of Honey as a Remedy for Allergic Diseases: A Mini Review. Front Pharmacol. 2021;11(1):2061-2070.
Al-kafaween MA, Hilmi ABM, Jaffar N, Al-Jamal HAN, Zahri MK, Jibril FI. Antibacterial and Antibiofilm activities of Malaysian Trigona honey against Pseudomonas aeruginosa ATCC 10145 and Streptococcus pyogenes ATCC 19615. Jordan J Biol Sci. 2020;13(1):69-76
Al-Farsi M, Al-Amri A, Al-Hadhrami A, Al-Belushi S. Color, flavonoids, phenolics and antioxidants of Omani honey. Heliyon. 2018;4(10):874-888
Ramanauskiene K, Stelmakiene A, Briedis V, Ivanauskas L, Jakštas V. The quantitative analysis of biologically active compounds in Lithuanian honey. Food Chem. 2012;132(3):1544-1548
Rao PV, Krishnan KT, Salleh N, Gan SH. Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Rev Bras Farmacogn. 2016;26(5):657-664
Liyanage D, Mawatha B. Health benefits and traditional uses of honey: A review. J Apith. 2017; 2(1):9-14.
Kuropatnicki AK, Kłósek M, Kucharzewski M. Honey as medicine: historical perspectives. J Apic Res. 2018; 57(1):113-118.
Al-Kafaween MA, Al-Jamal HAN, Hilmi ABM, Elsahoryi NA, Jaffar N, Zahri MK. Antibacterial properties of selected Malaysian Tualang honey against Pseudomonas aeruginosa and Streptococcus pyogenes. Iran J Microbiol. 2020; 12(6):565-576.
Khleifat KM, Qaralleh H, Al-limoun MO, Al-khlifeh EM, Aladaileh SA, Tawarah N, Almajali IS. Antibacterial and Antioxidant Activities of Local Honey from Jordan. Trop J Nat Prod Res. 2021;5 (3):470-477.
Rayyan WA, Alshammari SA, ALSammary AM, ALShammari MS, Seder N, Abu-Qatouseh LF. The Phytochemical Analysis and Antimicrobial Activity of Pergularia Tomentosa in North East Kingdom of Saudi Arabia KSA. Biomed Pharmacol. 2018;11(4):1763-1771.
Ahmed S and Othman NH. Honey as a potential natural anticancer agent: a review of its mechanisms. Evid-Based Compl Altern Med. 2013;2013:1-7.
Jull AB, Cullum N, Dumville JC, Westby MJ, Deshpande S, Walker N. Honey as a topical treatment for wounds. Cochrane Database Syst Rev. 2015;1(3):256-259.
Abd El-Malek FF, Yousef AS, El-Assar SA. Hydrogel film loaded with new formula from manuka honey for treatment of chronic wound infections. J Glob Antimicrob. 2017; 11:171-176.
Al-Nadaf AH, Seder NJ, Rayyan WA. Wound healing; antimicrobial and anti-oxidant activity for Jordanian Juglans Regia L. unripe fruits. J Innov Pharm. 2018;5(3):26-34.
Hoover SE and Ovinge LP. Pollen collection, honey production, and pollination services: managing honey bees in an agricultural setting. J. Econ. Entomol. 2018;111(4):1509-1516
Brosi BJ. The complex responses of social stingless bees (Apidae: Meliponini) to tropical deforestation. For Ecol. 2009; 258(9):1830-1837
Aksoy A, Ertürk YE, Erdogan S, Eyduran E, Tariq MM. Estimation of honey production in beekeeping enterprises from eastern part of Turkey through some data mining algorithms. Pak J Zool. 2018; 50(6):2199-2207.
Al-Ghamdi AA, Adgaba N, Herab AH, Ansari MJ. Comparative analysis of profitability of honey production using traditional and box hives. Saudi J Biol Sci. 2017; 24(5):1075-1080.
Erejuwa OO, Sulaiman SA, Ab Wahab MS. Honey: a novel antioxidant. Molecules. 2012; 17(4):4400-4423.
Dżugan M, Tomczyk M, Sowa P, Grabek-Lejko D. Antioxidant activity as biomarker of honey variety. Molecules. 2018; 23(8):2069-2077.
Stagos D, Soulitsiotis N, Tsadila C, Papaeconomou S, Arvanitis C, Ntontos A, Karkanta F, Adamou‑Androulaki S, Petrotos K, Spandidos DA. Antibacterial and antioxidant activity of different types of honey derived from Mount Olympus in Greece. Int J Mol Med. 2018; 42(2):726-734.
Avila S, Beux MR, Ribani RH, Zambiazi RC. Stingless bee honey: Quality parameters, bioactive compounds, healthpromotion properties and modification detection strategies. Trends Food Sci. Technol. 2018; 81:37-50.
Zafar M, Latafat T, Zehra A, Farooqui Y. Therapeutic Properties of Honey: A Review of Literature. Res Rev A JPharmacol. 2020;10(1):41-49.
Ng WJ, Chan YJ, Lau ZK, Lye PY, Ee KY. Antioxidant properties and inhibitory effects of trigona honey against Staphylococcus aureus planktonic and biofilm cultures. Int J. 2017; 12(37):28-33.
Ferreira IC, Aires E, Barreira JC, Estevinho LM. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem. 2009; 114(4):1438-1443.
da Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R. Honey: Chemical composition, stability and authenticity. Food Chem. 2016;196:309-323.
Helrich K. Official methods of analysis, vols. 1 and 2. (15th) ed. Arlington, VA: The Association; 1990. 64-85 p.
Ali F, Ranneh Y, Ismail A, Esa NM. Identification of phenolic compounds in polyphenols-rich extract of Malaysian cocoa powder using the HPLC-UV-ESI—MS/MS and probing their antioxidant properties. J Food ScinTechnol. 2015; 52(4):2103-2111.
Liu S-C, Lin J-T, Wang C-K, Chen H-Y, Yang D-J. Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chem. 2009; 114(2):577-581.
Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 2002;10(3):8-14.
Isla MI, Craig A, Ordoñez R, Zampini C, Sayago J, Bedascarrasbure E, Alvarez A, Salomón V, Maldonado L. Physico chemical and bioactive properties of honeys from Northwestern Argentina. LWT-Food Sci Technol. 2011;44(9):1922-1930.
Chua LS, Rahaman NLA, Adnan NA, Eddie Tan TT. Antioxidant activity of three honey samples in relation with their biochemical components. J Anal Methods Chem. 2013;2013:1-8.
USDA. Extracted Honey Grades and Standards, 1985. Accessed 4 May 2021 [Available from: https://www.ams.usda.gov/grades-standards/extractedhoney-grades-and-standards.
Moniruzzaman M, Sulaiman SA, Khalil MI, Gan SH. Evaluation of physicochemical and antioxidant properties of sourwood and other Malaysian honeys: a comparison with manuka honey. Chem Cent J. 2013;7(1):1-12.
Islam A, Khalil I, Islam N, Moniruzzaman M, Mottalib A, Sulaiman SA, Gan SH. Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year. BMC Compl Altern Med. 2012; 12(1):177-186.
Selvaraju K, Vikram P, Soon JM, Krishnan KT, Mohammed A. Melissopalynological, physicochemical and antioxidant properties of honey from West Coast of Malaysia. J Food Sci Technol. 2019; 56(5):2508-2521.
Madhu G, Kumar AS, Nair SK, editors. Sunlight-induced honey-mediated green synthesis of silver nanoparticles. AIP Conference Proceedings; AIP Publishing LLC. 2019.
Chirife J, Zamora MC, Motto A. The correlation between water activity and% moisture in honey: Fundamental aspects and application to Argentine honeys. J Food Eng. 2006;72(3):287-292.
Singh I and Singh S. Honey moisture reduction and its quality. J Food Sci Technol. 2018; 55(10):3861-3871.
Chuttong B, Chanbang Y, Sringarm K, Burgett M. Physicochemical profiles of stingless bee (Apidae: Meliponini) honey from South east Asia (Thailand). Food Chem. 2016; 192:149-155.
Al‐Mahasneh M, Rababah T, Ma'Abreh A. Evaluating the Combined Effect of Temperature, Shear Rate and Water Content on Wild‐Flower Honey Viscosity Using Adaptive Neural Fuzzy Inference System and Artificial Neural Networks. J Food Proc Eng. 2013; 36(4):510-520.
Chakir A, Romane A, Marcazzan GL, Ferrazzi P. Physicochemical properties of some honeys produced from different plants in Morocco. Arab J Chem. 2016; 9:S946-S954.
Saxena S, Gautam S, Sharma A. Physical, biochemical and antioxidant properties of some Indian honeys. Food Chem. 2010;118(2):391-397.
Mureşan CI, Buttstedt A. pH-dependent stability of honey bee (Apis mellifera) major royal jelly proteins. Sci Rep. 2019; 9(1):1-6.
Mateo R and Bosch-Reig F. Classification of Spanish unifloral honeys by discriminant analysis of electrical conductivity, color, water content, sugars, and pH. J Agric Food Chem. 1998; 46(2):393-400.
Alvarez-Suarez JM, Tulipani S, Díaz D, Estevez Y, Romandini S, Giampieri F, Damiani E, Astolfi P, Bompadre S, Battino M. Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food Chem Toxicol. 2010; 48(8-9):2490-2499.
Bertoncelj J, Doberšek U, Jamnik M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem. 2007; 105(2):822-828.
Sari DYI, Wijayanti HS, Afifah DN. Total phenolic content, antioxidant activity and organoleptic test of Nata de pina between various parts of honey pineapple variety (Ananas comosus [L.] Merr. Var. Queen). J Food Res. 2021; 5(4):169-174.
Mohamed M, Sirajudeen K, Swamy M, Yaacob M, Sulaiman S. Studies on the antioxidant properties of Tualang honey of Malaysia. Afr J Trad Compl Altern Med. 2010;7(1):59-63.
MartíNez RA, Schvezov N, Brumovsky LA, Román ABP. Influence of temperature and packaging type on quality parameters and antimicrobial properties during Yateí honey storage. J Food Sci Technol. 2017; 38:196-202.
Khalil MI, Mahaneem M, Jamalullail SMS, Alam N, Sulaiman SA. Evaluation of radical scavenging activity and colour intensity of nine Malaysian honeys of different origin. J ApiProd ApiMed Sci. 2011;3(1):04-11.
Zulkhairi Amin FA, Sabri S, Mohammad SM, Ismail M, Chan KW, Ismail N, Norhaizan ME, Zawawi N. Therapeutic properties of stingless bee honey in comparison with european bee honey. Adv Pharm Pharm Sci. 2018; 2018:1-12.
Abdulkhaliq A and Swaileh KM. Physico-chemical properties of multi-floral honey from the West Bank, Palestine. Int J Food Prop. 2017;20(2):447-454.
Santos-Buelga C and González-Paramás AM. Chemical composition of honey. Bee Products-Chemical and Biological Properties: Springer; 2017. 43-82 p.