The Potential Antioxidant Activity of N-Hexane Fraction of Mundar Leaves (Garcinia forbesii King.): doi.org/10.26538/tjnpr/v5i9.4

Sutomo Sutomo; Arnida Arnida; Syarifatul Lathifah; Rizki Aulia; Normaidah Normaidah

Authors

Sutomo Sutomo Department of Pharmaceutical Biology, Pharmacy Study Program, Universitas Lambung Mangkurat, Banjarbaru, Indonesia
Arnida Arnida Department of Pharmaceutical Biology, Pharmacy Study Program, Universitas Lambung Mangkurat, Banjarbaru, Indonesia
Syarifatul Lathifah Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat, Banjarbaru Indonesia
Rizki Aulia Pharmacy Study Program, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat, Banjarbaru Indonesia
Normaidah Normaidah Department of Pharmaceutical Chemistry, Pharmacist Professional Education Program, Universitas Lambung Mangkurat, Banjarbaru, Indonesia

Keywords:

Garcinia fobesii, Mundar, Extraction, Chromatography, Secondary Metabolites

Abstract

Mundar (Garcinia forbesii King.) is a native plant of South Kalimantan, Indonesia. This study was aimed to identify the compounds and evaluated the antioxidant activity of the n-hexane fraction and sub-fraction of G. forbesii leaves. The G. forbesii leaves were macerated with methanol. Liquid-liquid extraction of the methanol extract was performed using n-hexane-distilled water. The fraction obtained was then subjected to vacuum liquid chromatography using n-hexane:ethyl acetate gradient system with ratios of 25:1; 20:1; 15:1; 10:1; 8:2; 6:4; 4:6 and 2:8 v/v. Compound identification and qualitative antioxidant tests was done using thin-layer chromatography and then sprayed with specific reagents. The quantitative antioxidant tests were carried out using UV-Vis spectrophotometry. Maceration produced an extract with a yield of 20.4% and an n-hexane fraction of with a yeild of 29.0%. Fractionation with VLC resulted in 8 sub fractions (A-H). The qualitative test using specific reagents revealed that the 8 sub fractions (A-H) contained flavonoids and alkaloids, 2 fractions (B and C) contained terpenoids, 3 fractions (D-F) contained steroids, and 4 fractions (E-H) contained phenols and tannins. The qualitative antioxidant test showed that all sub fractions have potential antioxidant activity. Quantatively, the n-hexane fraction showed a strong antioxidant activity with an IC50 value of 26.07 ppm. The findings from this study shows that G. forbesii leaves have potential for use as a natural antioxidant agent.

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