Protective Effect of Aqueous Extract of Hypoestes rosea Against Indomethacin- Induced Gastric Ulcer in Sprague Dawley Rats
doi.org/10.26538/tjnpr/v6i6.28
Keywords:
Hypoestes rosea, Aqueous extract, Indomethacin, Gastric ulcer, Anti-ulcerAbstract
The leaves of Hypoestes rosea contain phytochemical compounds such as saponins, hypoestoxide, flavonoids and terpenoids amongst others. These compounds have been reported to be effective anti-diabetic, anti-ulcerogenic, anti-inflammatory and anti-oxidative agents. The leaves of H. rosea are also used in folk medicine for management of stomach related ailments. This study explores the effectiveness of aqueous leaf extract of the plant as anti-ulcerogenic potentials against indomethacin-induced gastric ulcer in Sprague Dawley rats. The LD50 of the crude extract was determined to be 3.25 g/kg bw. The rats were pretreated with 100, 200 and 300 mg/kg bw of the extract for 21 days prior to ulcer induction with indomethacin (40 mg/kg bw). The extract of H. rosea caused a significant (p<0.05) decrease in biochemical parameters of the gastric secretions analyzed as follows: Pepsin level of the gastric juice decreased from 126.30 ± 1.25 to 66.70 ± 1.20 µg/mL, gastric secretion volume decreased from 4.1 to 2.1 mL and the ulcer lesion index decreased from 13.40 to 1.67. Gross examination and histological investigation revealed that H. rosea extract prevented gastric mucosa erosion and hemorrhagic streaks, typical features of ulceration. It also prevented loss of mucin from the gastric mucosa. In conclusion, the results suggest that aqueous extract of H. rosea protects against indomethacin-induced gastric ulceration in Sprague Dawley rats with the most effective dose being 300 mg/kg
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