Antisecretory Effect of the Combination of Pegagan (Centella Asiatica) and Sambiloto (Andrographis paniculata) Leaves Ethanol Extracts on Pyloric Ligation-Induced Gastric Ulcer in Rats
doi.org/10.26538/tjnpr/v5i10.8
Keywords:
Antiseretory effect, Combination, Andrographis paniculata, Centella asiatica, Gastric ulcers, Pyloric ligationAbstract
Pegagan (Centella asiatica) and sambiloto (Andrographis paniculata) contain flavonoid compounds that can provide anti-ulcer effect. This study aims to investigate the antisecretory effect of the combination of C. asiatica and A. paniculata leaves ethanol extract on pyloric ligation-induced gastric ulcer in rats. The animals were treated with a combination of 100:75, 100:150, 200:75 and 200:150 mg/kg BW extract for 7 days after fasting for 48 hours prior to pyloric ligation. Furthermore, the animals were sacrificed 19 hours after pyloric ligation while the stomach was isolated for macroscopic, microscopic and gastric secretion studies. The combination of C. asiatica and A. paniculata leaves ethanol extract with 100:75, 100:150, 200:75, and 200:150 mg/kg BW doses showed antisecretory effect against ulcers induced by pyloric ligation. There was a significant difference (p < 0.05) between the combination of the extract and the control group in terms of ulcer score, ulcer and mucus indexes, volume, pH and acidity of gastric secretion, which showed a fair healing effect of 78.08%. Based on the results, the combination of C. asiatica and A. paniculata leaves ethanol extract have antisecretory effect with the most effective dose being 200:150 mg/kg BW.
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