Differentiation of Oil Palm Hybrid from Parentals Using As-PCR Marker
doi.org/10.26538/tjnpr/v1i6.7
Keywords:
Oil palm,, Tenera,, Dura,, Pisifera,, Allele Specific-PCR.Abstract
Oil palm (Elaesis guineensis) is a highly economic oil producing tree that produces fruits of three
forms which cannot be differentiated until reproductively matured. The commercially acceptable
form is the Tenera fruit form because of its high oil-yielding capacity. It is a hybrid of Dura and
Pisifera. The aim of this study was to determine alleles unique to Tenera fruit form of the Nigerian
oil palm. Allele Specific Polymerase Chain Reaction (AS-PCR) method was used in this study.
It required DNA isolation, common shell gene primer testing, allele specific primer pair investigation as right and left primers and a multiplex PCR investigation consisting of common and allele specific primers for the shell gene. These primer combinations were used to screen the genome of Dura, Pisifera and Tenera for variants in the shell gene. The findings from this study suggest two alleles of sizes 400 bp and 200 bp unique to Tenera when the primer combinations S3F&R and S22&S32 was used. This can serve as a marker for differentiating Tenera from Dura and Pisifera. The outcome of this research will aid early detection of choice variety for distribution
to Oil palm growers.
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