Antibacterial Activity and Molecular Docking Analysis of the Stem Bark Extracts of Persea americana Mill (Lauraceae) doi.org/10.26538/tjnpr/v6i6.27
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Abstract
Persea americana is utilized as food in most countries. Its excellent nutritional benefit and bioactivities have been extensively evaluated. Therefore, this study aims to determine the in-silico and in-vitro bioactivity of its bark extracts against multidrug-resistant (MDR) bacteria isolated from fish pond effluents. Extraction of the plant was done using the maceration method in ethanol, methanol, acetone, cold water, and hot water for 7 days and screened against MDRbacteria. Phytochemical screening was carried out on all the plant extracts. One hundred microliters of the crude extract were used for the minimum inhibitory concentration (MIC) test. Different bioactive molecules in the hot-water extract were identified using Gas chromatography-mass spectrometry (GC-MS) method and valuated using molecular docking tools to inhibit penicillin-binding protein (PBP) and DNA gyrase (DNAg). Phytochemicals such as tannins, flavonoids, saponins, steroids, alkaloids, and glycosides were present in all the extracts. Inhibition zones ranging from 0-20mm or methanol bark (MB), hot-water bark (HWB), and cold-water bark (CWB), ethanol bark (EB) (0-18mm), acetone bark (AB) 0-13mm at 100 mg/mL of the crude extract were observed. The MIC of the extracts against multi-drug resistant isolates in EB, MB, and HWB were at 6.25 mg/mL, while those of AB and CWB were at 12.5 mg/mL and 12.5 mg/mL, respectively. GC-MS analysis showed the presence of 105 compounds. Likewise, molecular docking revealed that certain phytoconstituents examined in this study had higher binding affinity compared to conventional antibiotics. Therefore, P.americana bark possesses promising bioactive chemicals that can be used as antibacterial agents.
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Adwan G, Abu-Shana B, Adwan K. antibacterial activities of some plant extracts alone and in combination with different antimicrobials against multidrug-resistant Pseudomonas aeruginosa strains. Asian Pac J. Trop Med. 2018; (3):266-269.
Alves MJ, Froufe HJC, Costa AFT, Santos AF, Oliveira, LG, Osório, SRM. Docking studies in target proteins involved in antibacterial action mechanisms: extending the knowledge on standard antibiotics to antimicrobial mushroom compounds. Mol. 2016; 19:1672-1684.
Kroemer RT. structure-based drug design: docking and scoring. Curr Protein Pep Sci. 2007; 8:312-328.
Dos-santos, FJB, Moura, DJ, Peres, YF, Sperotto, Arm, Caramão, EB. Genotoxic, and mutagenic properties of bauhinia platypetala extract, a traditional Brazilian medicinal plant. J. Ethnopharmacol. 2011; 44:474-482.
Maitera ON, Osemeahon SA, Barnabas HL. Proximate and elemental analysis of avocado fruit obtained from Taraba state, Nigeria. Ind J Sci Res Technol. 2014; 2(2):67-73.
Tassew BB, Zinabwa HT, Eyasu Gebrie A. A Review on Avocado Seed: Functionality, Composition, Antioxidant and Antimicrobial Properties. Chem Sci Int J. 2019; 27(2):1-10.
Raúl VL, Dariana GR, Rocío I. Díaz DG, María G, Alejandro C, Adriana P, Carmen HB. Purified avocado seed acetogenins: Antimicrobial spectrum and complete inhibition of Listeriamonocytogenes in a refrigerated food matrix, CYTA. J Food. 2019; 17(1):228-239.
Trease GE and Evans WC. Pharmacognosy. 13th (ed). ELBS/Bailliere Tindall, London. 1989. 345-346 p, 535-536 p, 772-773p.
Sofowora A. Medicinal plants and Traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. 1993. 289 p.
Doughari JH, Pukuma MS, De N. Antibacterial effects of Balanites aegyptiaca L. Drel. and Moringa oleifera Lam. On Salmonella typhi. Afr J Biotechnol. 2007; 6(19):2212-2215.
Ekaiko M, Arinze A, John W, Ajah O. Antimicrobial effect of the leaf extract of Psidium guajava L. and Carica papayaL. Int J Life Sci Res. 2016; 3:55-60.
Emmanuel EU, Onagbonfeoana ES, Chinedu NP, Chibuike AO, Edith OC, Chioma I, Obinna A, Gavin IC, Raymond IC, Ndukaku OY. meliorative effect of methanol extract of Telfairia occidentalis Hook. and Amaranthus hybridus Linn. Against cadmium-induced oxidative stress in rats. J Appl Pharm Sci, 2017; 7(09):109-115.
Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical screening of stem bark extract from Jatropha curcas (Linn). Afr J Pharm Pharmacol. 2019; 3(2):058-062.
Ogundare AO and Oladejo BO. Antibacterial Activities of the Leaf and Bark Extract of Persea americana. Am J Ethnomed. 2017; 1(1):064-071.
Amoussatou S, Eléonore YL, Louis F, Kamirou CS, Fidèle A, Joachim DG. Comparative phytochemical analysis and antimicrobial activity of extracts of seed and leaf of Persea americana Mill. Acad J Med Plant Res. 2020; 8(5):058-063.
Khan HR, Sikandar B, Tasveer N, Kanwal GS, Kazmi S. Qualitative phytochemical screening and antifungal activity of Carica papaya leaf extract against human and plant pathogenic fungi. Int Res J Pharm. 2017; 4:83-86.
Nurdin R, Nikmah UD, Bohari O. Phytochemical and Antioxidant Activity of Avocado Leaf Extract (Persea americana Mill.). Asian J Sci Res. 2018; 11:357-363.
Oboh G, Odubanjo VO, Bello F, Ademosun AO, Oyeleye SI, Nwanna EE, Ademiluyi AO. Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro. J Basic Clin Physiol Pharmacol. 2016; 27(2):131-140.
Umar H, Bashar I, Usman B, Alhassan O, Ahmed H, Labaran, I, Bello A. Phytochemical Screening of Aqueous Extract of Garlic (Allium sativum) bulbs. Rep Opin. 2018; 6(8):1-4.
Okigbo R, Okorie R, Putheti R. In vitro effects of garlic (Allium sativum L.) and African basil (Ocimum gratissimumL.) on pathogens isolated from rotted cassava roots. Interciencia. 2019; 34:742-747.
Raquel FE. Bacterial lipid composition and antimicrobial efficacy of cationic steroid compounds. Biochem Biophy Acta. 2017; 1778:2500-2509.
Okwu DE. Evaluation of the chemical composition of indigenous spices and flavoring Agents. Glob J Pure Appl Sci. 2017; 7(3):455-459.
Rao V and Sung MK. Saponin as anticarcinogens. The J Nutr. 2015; 125:717S-724S.
Nyarko AA and Addy ME. Effects of aqueous extract of Adenia cissampeloides on blood pressure and serum analyte of hypertensive patients. Phytother Res. 2016; 4(1):25-28.
Achi NO, Chimaraoke C, Ekeleme E, Onyeanula, JC. Phytochemical, proximate analysis, vitamin and mineral composition of aqueous extract of Ficus capensis leaves in South-Eastern Nigeria. The J Nutr. 2017; 7:117-122.
Chowdhury R. Pharmacological Investigation of Leaves of Carica papaya. (Doctoral dissertation, East-West University). 2014.167-180
Aruljothi S, Uma C, Sivagurunathan P, Bhuvaneswari M. Investigation on antibacterial activity of Carica Papaya Leaf Extracts against Wound infection-causing Bacteria. Int J Res Stud Biosci. 2016; 2(11):8-12.
Al-Anazi, K and Al-Jasser A. Infections Caused by Stenotrophomonas maltophilia in Recipients of Hematopoietic Stem Cell Transplantation. Front Oncol. 2016; 4:232.
Shibula K and Velavan S. Determination of Phytocomponents in methanolic extract of Annona muricata leaf using GC-MS technique. Int J Pharmacogn Phytochem Res. 2015; 7(6):1251-1255.
Komansilan A, Abadi AL, Yanuwiadi B, Kaligis DA. Isolation and identification of biolarvicide from Soursop (Annona muricata Linn) seed to mosquito (Aedes aegypti) larvae. Int J Eng Technol. 2016; 12(03):28-32.
Sermakkani M and Thangapandian V. GC-MS analysis of Cassia italica leaf methanol extract. Asian J Pharm Clin Res. 2016; 5(2):90-94.
Kumar P, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res. 2016; 4(7):191-195.
Mukhtar M, Arshad M, Ahmad RJ, Pomerantz B, Wigdahl B, Parveen Z. Antiviral potentials of medicinal plants. Virus Res. 2018; 131(2):111-120.
Denaro M, Smeriglio A, Barreca D, De Francesco C, Occhiuto C, Milano G, Trombetta D.Antiviral activity of plants and their isolated bioactive compounds: an update. Phytother Res. 2020; 34(4):742-768.
Lipinski CA. “Lead-and drug-like compounds: the ruleoffice revolution,” Drug Discov Today: Technol. 2004; 1(4):337-341.
Benet LZ, Hosey CM, Ursu O, Oprea TI. “BDDCS, the rule of 5 and d