Physiological and Histopathological Effects of Two Seed Extracts (Phoenix dactylifera and Raphanus sativus) and L-Carnitine Drug on Semen Quality and Testicular Sexual Hormonal Changes in Adult Male Rabbits doi.org/10.26538/tjnpr/v5i10.3
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Abstract
Date palm (Phoenix dactylifera) pollen (DPP) is widely used in traditional medicine to treat male infertility. Radish (Raphanus sativus) is an edible root vegetable that was domesticated in Europe in pre-Roman times. This study was aimed at comparing the effects of DPP and Radish seed extracts, as well as L-Carnitine drug on the quality of sperm and sex hormone parameters in adult male rabbits. A total of 30 male rabbits were divided into five groups. Each group was administered with: normal saline (Control 1; Group I), DPP (Group II), L-Carnitine (Group III), Petroleum ether (Control 2; Group IV), or Radish seed oil extract (Group V) every day for eight weeks. Semen quality, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured at the end of the experiment. Also, histopathological examinations on testicles were performed. The results showed that there was significant increase in activity, total and normal sperm counts in Group II compared to Groups I and III. Meanwhile, a significant increase in activity, total and normal sperm counts was observed in Groups V compared to IV. The hormonal levels of FSH, LH, and testosterone were significantly higher in Groups II and III compared to Group I, but significantly lower in Groups V compared to IV. The findings of this study revealed that date palm pollen suspension seems to improve sperm quality, enhance fertility in male adult rabbits compared to the Radish seed oil extract and L-Carnitine drug, therefore, it may be useful in solving infertility problems.
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References
Hassan AM, Alam SS, Abdel-Aziem SH, Ahmed K. A.Benzo-a-pyrene induced genotoxicity and cytotoxicity in germ cells of mice: Intervention of Radish and cress. J Genet Eng Biotechnol. 2011; 9(1):65-72.
Baltrušaitytė V, Venskutonis PR, Čeksterytė V. Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chem. 2007; 101(1):502-514.
Özcan M. Inhibition of Aspergillus parasiticus NRRL 2999 by pollen and propolis extracts. J Med Food. 2004; 7(1):114-116.
Campos MG, Webby RF, Markham KR, Mitchell KA, Da Cunha AP. Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids. J Agric Food Chem. 2003; 51(3):742-745.
Leja M, Mareczek A, Wyżgolik G, Klepacz-Baniak J, Czekońska K. Antioxidative properties of bee pollen in selected plant species. Food chem. 2007; 100(1):237-240.
Choi Eun‐Mi. Antinociceptive and anti-inflammatory activities of pine (Pinus densiflora) pollen
extract. Phytother Res. 2007; 21(5):471-475.
Hammed MS, Arrak JK, Al-Khafaji NJ, Hassan AA. Effect of date palm pollen suspension on ovarian function and fertility in adult female rats exposed to lead acetate. DJM.2012; 3(1):90-96.
Uzbekova DG, Makarova VG, Khvoynitskaya LG, Slepnev AA. Evaluation of bee-collected pollen influence on lipid peroxidation, antioxidant system and liver function in old animals. J Hepatol. 2003; 38(1):203.
Adaay MH and Mattar AG. Effect of aqueous and ethanolic extracts of Tribulus terrestris, Phoenix dactylifera and Nasturtium officinale mixture on some reproductive parameters in male mice. Baghdad Sci J. 2012; 9(1):640-50.
Phillipson JD. Phytochemistry and medicinal plants. Phytochem. 2001; 56(3):237-243.
Ali BH, Bashir AK, Alhadrami G. Reproductive hormonal status of rats treated with date pits. Food Chem. 1999;66(4):437-441.
Salah-Abbès JB, Abbès S, Abdel-Wahhab MA, Oueslati R. Raphanus sativus extract protects against zearalenone induced reproductive toxicity, oxidative stress and mutagenic alterations in male Balb/c mice. Toxicon. 2009; 53(5):525-533.
El-Tohamy, Magda M, El-Nattat WS, El-Kady RI. The beneficial effects of Nigella sativa, Raphanus sativus, and Eruca sativa seed cakes to improve male rabbit fertility, immunity, and production. Am J Sci. 2010; 6(10):1247-1255.
Anders K, Oegaard J, Yasuhiko M, Yoshio O, Yasushi M. Volatiles in distillates of fresh radishes of Japanese and Kenyan origin. Agric Biol Chem.1978; 42(9):1715-1721.
Ruiz‐Pesini E, Alvarez E, Enríquez JA, López‐Pérez MJ. Association between seminal plasma carnitine and sperm mitochondrial enzymatic activities. Int J Androl. 2001; 24(6):335-340.
Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A, Gharsallah N. Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of Date Palm Pollen (DPP) from two Tunisian cultivars. Arab J Chem. 2019; 12(8):3075-3086.
Sharma A, Khare SK, Gupta MN. Enzyme‐assisted aqueous extraction of peanut oil. J Am Oil Chem Soc. 2002;79(3):215-218.
Waheed A, Hamid FS, Madiha B, Seemab, Naveed A, Nadia K, Hina G. GC-MS analysis of chemical components seed oil of Raphanus sativus L. MOJ Toxicol. 2019;5(3):112-118.
Bahmanpour S, Panjeh SM, Talaei T, Vojdani Z, Poust PA, Zareei S, Ghaemian M. Effect of Phoenix dactylifera pollen on sperm parameters and reproductive system of adult male rats. Iran J Med Sci. 2006; 21(1):208-212.
Khushboo M, Murthy MK, Devi MS, Sanjeev S, Ibrahim KS, Kumar NS, Gurusubramanian G. Testicular toxicity and sperm quality following copper exposure in Wistar albino rats: ameliorative potentials of L-carnitine. Environ Sci Pollut Res. 2018; 25(2):1837-1862.
Abed-Alazeez LA, Ali AH, Haba MK. The Protective Effect of Radish (Raphanus sativus) Seeds against the Oxidative Stress Induced by Sodium Nitrite in Male Rabbits Oryctolagus cuniculus. Baghdad Sci J. 2016; 13(1):44-51.
Véras RML, Ferreira MDA, De Carvalho FFR, Véras ASC. Forage cactus (Opuntia ficus-indica Mill) meal in replacement of corn; 1: apparent digestibility of nutrients. Rev Bras de Zootec. 2002; 28(2):145-152.
Finley PR and Tietz NW. Clinical guide to laboratory tests. WBSC. 1996; 8(1):123-125.
Suvarna KS, Christopher L, Bancroft JD. BTAP of Hist Tech 7th Edition. 2013; 6(2):395-405.
Schwartz BM, Janie H, Wilson DM. Goff. An easy guide to research design & SPSS. SAGE Open Med. 2018; 17(2): 93-103.
Faleh BH and Alaa AS. Effect of palm pollen grains extracts Phoenix dactylifera L. on the spermatogenic activity of male rabbits. BJR. 2006; 5(1-2):1-10.
Yoshida M, Erik F, Michael EH. mGluR‐dependent persistent firing in entorhinal cortex layer III neurons. Eur J Neurosci. 2008; 28(6):1116-1126.
Purdy PH, Ericsson SA, Dodson RE, Sternes KL, Garner DL. Effects of the flavonoids, silibinin and catechin, on the motility of extended cooled caprine sperm. Small Rumin. Res. 2004; 55(1-3):239-243.
Hassan HMM. Chemical composition and nutritional value of palm pollen grains. Glob J Biochem. 2011; 6(1):1-7.
Jacyno E, Kołodziej A, Kamyczek M, Kawęcka M, Dziadek K, Pietruszka A. Effect of L-carnitine supplementation on boar semen quality. Acta Vet Brno. 2007; 76(4):595-600.
Rebouche CJ and Hermann S. Carnitine metabolism and its regulation in microorganisms and mammals. Annu Rev Nutr. 1998; 18(1):39-61.
Jeulin C and Lawrence ML. Role of free L-carnitine and acetyl-L-carnitine in post-gonadal maturation of mammalian spermatozoa. Hum Reprod. 1996; 2(2):87-102.
Abdel-Hamed OA, El-Sayed AI, Iraqi MM, Saad AM,Radwan AA. Comparative evaluation of different herbal formula and L-Carnitine on reproductive performance of male Moshtohor rabbits. Anim Biotechnol. 2014;11(17):11.
Zaman R. "Study of cardioprotective activity of Raphanus sativus L. in the rabbits." Pak J Biol Sci. 2004; 7(5):843-847.
Matsufuji H, Otsuki T, Takeda T, Chino M, Takeda M. Identification of reaction products of acylated anthocyanins from red Radish with peroxyl radicals. J Agric Food Chem. 2003; 51(10):3157-3161.
El-Tohamy MM, El-Nattat WS, El-Kady RI. The beneficial effects of Nigella sativa, Raphanus sativus, and Eruca sativa seedcakes to improve male rabbit fertility, immunity, and production. Am J Sci. 2010; 6(10):1247-1255.
Melis MR, Alessandro M, Antonio A. Apomorphine-and oxytocin-induced penile erection and yawning in intact and castrated male rats: effect of sexual steroids.Neuroendocrinol.1994; 59(4):349-354.
Kostyuk VA, Potapovich AI, Strigunova EN, Kostyuk TV,Afanas' Ev IB. Experimental evidence that flavonoid metalcomplexes may act as mimics of superoxide dismutase. Arch Biochem Biophys. 2004; 428(2):204-208.
Zargar AH, Wani AI, Masoodi, SR, Laway BA, Bashir MI, Salahuddin M. Epidemiologic and etiologic aspects of hirsutism in Kashmiri women in the Indian subcontinent. Fertil Steril. 2002; 77(4):674-678.
Bhat GM, Lone MI, Alsolami S, Iqbal QM. Recurrent malignant Leydig cell tumor of testis: a case report with review of literature. Gulf J Oncol. 2010; 7(1):42-45.
Austin CR and Short RV. Reproduction in Mammals: Books 1 to 5. CUP. 1972(4); 440-462.
Peluso G, Barbarisi A, Savica V, Reda E, Nicolai R, Benatti P, Calvani M. Carnitine: an osmolyte that plays a metabolic role. J Cell Biochem. 2001; 80(1):1-10.
Babu SR, Sadhnani MD, Swarna M, Padmavathi P, Reddy PP. Evaluation of FSH, LH and testosterone levels in different subgroups of infertile males. Indian J Clin Biochem. 2004; 19(1):45-49.
Mehraban F, Jafari M, Toori MA, Sadeghi H, Joodi B, Mostafazade M, Sadeghi H. Effects of date palm pollen Phoenix dactylifera L. and Astragalus ovinus on sperm parameters and sex hormones in adult male rats. Iran J Reprod Med. 2014; 12(10):705.
Midzak AS, Chen H, Papadopoulos V, Zirkin BR. Leydig cell aging and the mechanisms of reduced testosterone synthesis. Mol Cell Endocrinol. 2009; 299(1):23-31.
Neuman SL, Lin TL, Heste PY. "The effect of dietary carnitine on semen traits of White Leghorn roosters." Poult Sci J. 2002; 81(4):495-503.
Adabi SG, Cooper RG, Ceylan N, Corduk M. L-carnitine and its functional effects in poultry nutrition. Poult Sci J. 2011; 67(2):277-296.
Morris L and Gibb Z. Oral supplementation with Lcarnitine improves stallion fertility. J Equine Vet Sci. 2016; 43(1):S82.