Cytotoxic Activity of Two Compounds Extracted from Artocarpus elasticus Reinw. Ex Blume Leaves on Selected Cancer Cell Lines
doi.org/10.26538/tjnpr/v5i10.5
Keywords:
Artocarpus elasticus Reinw. Ex Blume, apigenin, 6-prenyl apigenin, anticancerAbstract
Artocarpus elasticus Reinw. Ex Blume is a genus in the Moraceae family and it contains an abundance of phenolics, such as flavonoids, stilbenoids, dihydrochalcones, dihydrobenzoxanthones, and prenylated flavonoids. This study was aimed at isolating chemical constituents from Artocarpus elasticus leaves and investigate the cytotoxic effect of the isolated compounds on selected cancer cell lines. Extract was prepared from A. elasticus leaves, which was followed by fractionation and isolation of chemical constituents via chromatographic technique. The chemical structures of the two compounds obtained from the leaf extract were established using several spectroscopic methods. Cytotoxic effect of both compounds against P388, MCF-7, and T47D cancer cells was investigated using the MTT assay. The results revealed that the two compounds isolated from the leaves of A. elasticus were apigenin and 6-prenyl apigenin. The in vitro cytotoxicity assay showed IC50 values of 14.13, 40.35, and 434.20 g/mL and 6.65, 19.75, and 313.61 g/mL for apigenin and 6-prenyl apigenin, respectively. This observation demonstrates that 6-prenyl apigenin displayed higher cytotoxic activity compared to apigenin. The finding of this research suggests that both compounds might be useful as anticancer agents.
References
Gupta AK, Pathak U, Medhi M, Mastinu A, Sikarwar MS, Mishra P. Botanical chemical and pharmacological properties of Artocarpus lakoocha (Monkey Fruit): A Review. Agric Rev. 2020; 41(4):305-316.
Daus M, Chaithada P, Phongpaichit S, Watanapokasin R, Carroll AR, Mahabusarakan W. New prenylated dihydrochalcones from the leaves of Artocarpus elasticus. Phytochem Lett. 2017; 19:226-230.
Ramli, F, Rahmani M, Ismail IS, Sukari, MA, Rahman MA, Zajmi A, Akim AM, Hashim NM, Go R. A new bioactive secondary metabolite from Artocarpus elasticus. Nat Prod Commun. 2016; 11(8):1103-1106.
Jenis J, Baiseitova A, Yoon SH, Park C, Kim JY, Li ZP, Lee KW, Park KH. Competitive α-glucosidase inhibitors, dihydrobenzoxanthone, from the bark of Artocarpus elasticus. J Enzy Inhib Med Chem. 2019; 34(1):1623-1632.
Chan EWC, Wong SK, Tangah J, Chan HT. Chemistry and Pharmacology of Artocarpin: An isoprenyl flavone from Artocarpus spesies. Sys Rev Pharm. 2018; 9(1):58-63.
Ye JB, Ren G, Li WY, Zhong GY, Zhang M, Yuan JB, Lu T. Characterization and identification of prenylated flavonoids from Artocarpus heterophyllus Lam. roots by Quadrupole Timeof-Flight and Linear Trap Quadrupole Orbitrap Mass Spectrometry. Molecules. 2019; 24(24):4591.
Mustapha I, Juliawaty LD, Syah YM, Hakim EH, Latip J, Ghisalberti EL. An oxepinoflavone from Artocarpus elasticus with cytotoxic activity against P-388 Cells. Arch Pharm Res.2009; 32(2):191-194.
Salahuddin S, Megawati M, Fajriah S. Screening of phytochemistry and toxicity assay of Artocarpus elasticusleaves. Proc Nat Sem Food Med Environ Res Health. 2013; 457-463p.
Megawati M, Fajriah S, Darmawan A, Salahuddin S. Antioxidant activity of Aktivitas Artocarpus elasticus BL. (Moraceae) leaves extracts. Nat Proc Indon Appl Chem. 2013;1:12-15.
Harneti D, Tjokronegoro R, Safari A, Supratman U, XeMin L, Mukhtar MR, Mohamad K, Awang K, Hayashi H. Cytotoxic triterpenoids from the bark of Aglaia smithii (Meliaceae). Phytochem Lett. 2012; 5:496-499.
Yunianto P, Rosmalawati S, Rachmawati I, Suwarso WP,Sumaryono W. Isolation and identification of endophytic fungi from Srikaya plants (Annona squamosa) having potential secondary metabolites as anti-breast cancer activity. J Microb Indonesia. 2012; 6:23-29.
Susidarti RA, Jenie RI, Ikawati M, Putri DDP, Meiyanto E. Cytotoxic activity and apoptosis induction of 8-hydroxyisocapnolactone-2,3-diol and its combination with doxorubicin on MCF-7 and T47D cells. J Appl Pharm Sci. 2014; 4(06):089-097.
Fajriah S, Megawati M, Darmawan A. Apigenin, an anticancer isolated from Macaranga gigantifolia leaves. J Trop Life Sci. 2016; 6(1):7-9.
Beula SJ, Raj BA, Mathew B. Isolation and molecular recognization of 6-prenyl apigenin towards MAO-A as the active principle of seeds of Achyranthes aspera. Biomed Prev Nutr. 2014; 4(3):379-382.
Arung ET, Yoshikawa K, Shimizu K, Kondo R. Isoprenoidsubstituted flavonoids from wood of Artocarpus heterophyllus on B16 melanoma cells: cytotoxicity and structural criteria. Fitoter. 2010; 81:120-123.
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
