Eco-Friendly and Cost-Effective Method for the Quantitative Determination of Ceftriaxone Sodium in Pharmaceutical Drug Formulations doi.org/10.26538/tjnpr/v5i10.4
Main Article Content
Abstract
Ceftriaxone sodium is a beta-lactam antibiotic with bacteriocidal activity. This drug is eliminated in the urine, although only in a 33-67% concentration. Many High-Performance Liquid Chromatography (HPLC)-based methods for the analysis of ceftriaxone have been developed using various solvents, but these solvents are expensive, poisonous, and harmful. This study was therefore aimed at developing an eco-friendly and cost-effective HPLC-based approach for the quantitative determination of ceftriaxone sodium in pharmaceutical drug formulations. In the method development, the column used was X-Bridge C-18 (4.6 mm x 250 mm, 5 µm) for the separation, identification, and determination. The mobile phase was a mixture of phosphate buffer and methanol in the ratio of 73:27 (% v/v) at a flow rate of 1 mL/min, while the temperature was maintained at 25oC. Throughout the method development, the photodiode array detector was at a wavelength of 254 nm. The developed approach demonstrated good system applicability, with an RSD of less than 2%. R2 was 0.9998, which is exact because the % RSD is less than 2% (repeatability with 0.5% RSD and intermediate precision with 0.22% RSD). The limit of detection (LOD) and limit of quantification (LOQ) for the method were 0.0472 and 0.157 mg/mL, respectively. The R2 and RSD values suggested that the approach was linear, exact, and accurate, demonstrating that the developed method is environmentally friendly, cost-effective, and green.
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