The Effect of Exposure to Polystyrene Nanoplastics on Cytokine Levels and Reproductive System of Male Tilapia
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Abstract
Several studies demonstrated adverse effects associated with exposure to polystyrene nanoplastics (NPs), such as toxicity, inflammation, or other health-related concerns. This study aims to analyse the impact of NPs exposure on pro-inflammatory cytokine levels and testicular histology in tilapia gonads. Twenty-four male tilapias were used in this study and divided into four groups, including a control (commercial pellets only) and three treatment groups (commercial feed mixed 0.5, 1.0 and 2.0 µL/kg of NPs, respectively) for 25 days. Blood sample was used to tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-ɣ) levels analysis. Histology of testicular tissue was prepared with hematoxylin and eosin (HE) staining. The results showed that NPs exposure has no significant influence of TNF-α and IFN-ɣ levels. The levels of TNF-α on the control (1.6 ± 0.3 ng/mL) and the treatment groups from low to high concentration (1.7± 0.4; 1.7 ± 0.4; and 1.6 ± 0.3 ng/mL) and the levels of IFN-γ control (16.7 ± 2.8 ng/mL) and the treatment groups (19.1 ± 5.3; 18.7 ± 4.2; and 19.8 ± 3.5 ng/mL). Polystyrene nanoplastics exposure can reduce the number of spermatogenic cells. Spermatogonia cells was decreased from 27.5 ± 0.9 to 13.1 ± 1.1 cells/cyst. Spermatocyte cell was decreased from 57.6 ± 0.6 to 18.5 ± 0.3 cells/cyst. Spermatid cells was decreased from 86.8 ± 2.7 to 38.5 ± 2.7. In conclusion, exposure to multiple doses of NPs for 25 days did not increase pro-inflammatory cytokines levels in the blood serum of tilapia, but it caused histological changes in the gonads of fish.
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