Gotu Kola Supplement Ameliorates Stress-Induced Liver Injury in Mice: doi.org/10.26538/tjnpr/v6i6.25

Anthony  T. Eduviere; Mamerhi T. Enaohwo; Prosper E. Awhin; Lily O. Otomewo; Omogor Iwalio

Authors

Anthony T. Eduviere Department of Pharmacology, Delta State University, Abraka, Nigeria
Mamerhi T. Enaohwo Department of Human Anatomy & Cell Biology, Delta State University, Abraka, Nigeria
Prosper E. Awhin Department of Medical Biochemistry, Delta State University, Abraka, Nigeria
Lily O. Otomewo Department of Pharmacology, Delta State University, Abraka, Nigeria
Omogor Iwalio Department of Pharmacology, Delta State University, Abraka, Nigeria

Keywords:

Sleep deprivation, Oxidative stress, Liver function, Gotu kola

Abstract

Insufficient sleep is a common lifestyle which induces stress and has been shown to enhance the generation of free radicals, thus resulting in cellular injury. Gotu kola is a medicinal plant with established antioxidant profile. This study aims to evaluate the probable ameliorative effect of Nigerian-processed Gotu kola supplement known as Centella lujica on liver function in mice subjected to stress initiated by sleep disruption. Thirty mice (22+2g) were randomly assigned into five groups. Treatment lasted for seven days. Group 1 served as control (not sleep deprived), Group 2 was sleep deprived, mice in groups 3-5 in addition to being sleep deprived for the final 72 h of treatment, received Centella lujica (50 mg/kg), (100 mg/kg), astaxanthin (50mg/kg) respectively. Liver enzyme activity and hepatic oxidative stress parameters were assessed thereafter. Hepatocellular changes were also recorded using histological techniques. Pre-treatment of sleep-deprived mice with Centella lujica supplement caused a reduction in pro-oxidants with a concurrent increase in antioxidant levels. Meanwhile, the opposite effect was the case in the sleep-deprived group. Also, liver enzyme activity and liver cell degradation were significantly (p <0.05) reduced in the centella groups when compared to the sleep deprived group. In conclusion, Centella lujica supplement caused a reduction in oxidative stress and enhanced liver function in mice exposed to 72 h sleep restriction.

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