Haematological and Biochemical Examination of Pulcherrimin A isolated from Caesalpinia pulcherrima stem bark doi.org/10.26538/tjnpr/v5i11.19

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Published: Nov 1, 2021
Keywords:
Silver nanoparticles, Biosynthesis, Antibacterial activity, Gnetum africanum, Anacardium occidentale, Landolphia owariensis

Main Article Content

Marie E. U. Dibua
Department of Microbiology, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
Joseph Ukomadu
Department of Microbiology, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
Emmanuel S. Okeke
Department of Biochemistry, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
Onome Ejeromedoghene
School of Chemistry and Chemical Engineering, Southeast University, Jiangning District, Nanjing, Jiangsu Province, 211189, PR China.
Stephen C. Emencheta
Department of Pharmaceutical Microbiology and Biotechnology, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
Elibe I. Mba
Department of Microbiology, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria

Abstract

The rapid increase in microbial infections and the failure of conventional therapeutic agents (antibiotics) are currently a global public health threat. However, nanoparticles can be used as a potential treatment alternative. This study investigated the antimicrobial potential of silver nanoparticles (AgNps) biosynthesized from culture cell-free supernatant of Aspergillus flavus (Af) and aqueous extract of plants: Gnetum africanum (Ga), Anacardium occidentale (Ao), and Landolphia owariensis (Lo). The AgNps were synthesized and characterized respectively, using standard procedures. Their antimicrobial efficacy were determined against Staphylococcus spp, Klebsiella spp., and Salmonella spp. using the agar well diffusion method with ciprofloxacin (CPX) as a positive control. The AgNps were confirmed by visible colour change in the reaction mixtures of the AgNO3 solution and the samples. The characterization of the AgNps through UV-Visible spectroscopy showed that Ao-AgNps, Lo-AgNps, Ga-AgNps, and Af-AgNps had maximum absorbance at a wavelength of 439, 437, 428, and 453 nm respectively which correspond to the excitation of surface plasmon resonance (SPR). SEM and TEM studies revealed irregular spherical nanoparticles with sizes around 100 nm. The aqueous leaf extract synthesized AgNps displayed low antibacterial activity (P < 0.05) (possibly due to environmental conditions) against the test organisms compared to CPX except for Ga-AgNps with a substantially high inhibitory effect against Klebsiella spp. only. More so, the fungal biomass (Af-AgNPs) synthesized AgNps displayed substantial inhibitory efficacy against all the tested pathogens. This study reveals the potential antimicrobial abilities of the AgNps of the fungus and plant extracts.

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How to Cite
E. U. Dibua, M., Ukomadu, J., S. Okeke, E., Ejeromedoghene, O., C. Emencheta, S., & I. Mba, E. (2021). Haematological and Biochemical Examination of Pulcherrimin A isolated from Caesalpinia pulcherrima stem bark: doi.org/10.26538/tjnpr/v5i11.19. Tropical Journal of Natural Product Research (TJNPR), 5(11), 2005-2010. https://tjnpr.org/index.php/home/article/view/347
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Vol. 5 No. 11 (2021): Tropical Journal of Natural Product Research
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Articles
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Author Biography

Emmanuel S. Okeke, Department of Biochemistry, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria

Natural Science Unit, SGS, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria

Institute of Environmental health and Ecological Security, School of Environment and Safety Engineering, Jiangsu University, 212013 P.R. China

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