Anticonvulsant Activity of Hippocratea welwitschii Oliv. (Celastraceae) Root Extracts on Chicks and Swiss Mice http://www.doi.org/10.26538/tjnpr/v8i1.48
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Abstract
Dried roots of Hippocratea welwitschii are a prevalent traditional medication for the management of epilepsy in the traditional ‘Ukanafum’ system of medicine in the Akwa Ibom, South Southern, Nigeria. Various extracts of the root of the plant namely: n-hexane, ethyl acetate, methanolic, aqueous and crude extracts were subjected to anticonvulsant evaluation using the maximal electroshock test (MEST) in chicks, subcutaneous pentylenetetrazole (scPTZ) and strychnine induced seizures test in mice. The neurotoxic potential of the extracts was assessed using beam walking assays in mice. The n-hexane (250 mg/kg), methanol (250 mg/kg) and aqueous (125 and 250 mg/kg) extracts produced 30% protection against seizure induced by MEST. The methanol extract (250 mg/kg) and crude extract (125 mg/kg) showed 80% and 70% protection in the scPTZ test, respectively. Similarly, the aqueous extract at 125 mg/kg protected 60% of the mice against seizure induced by strychnine. In the beam walking assay test, only the methanol extract produced a significant (p < 0.05) increase in the number of foot slips and the time taken to complete the task. In conclusion the various extracts of the root of Hippocratea welwitschii demonstrated anticonvulsant activity and deserve further study on the isolation and elucidation of the anticonvulsant active compounds and possible mechanism of anticonvulsant action.
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Muhammad KJ, Jamil S, Basar N, Magaji MG.
Anticonvulsant studies on the isolated compounds from the
leaves of Scurrula parasitica L (Loranthaceae). Malaysian J
Fund Appli Sci. 2019; 15(6): 806-810.
Yemitan OK, Adeyemi OO. Antiepileptogenic and
anticonvulsant actions of Dalbergia saxatilis (Hook, F.) in
sub-toxic chemical kindling and toxic convulsant models.
Eur J Med Plants. 2013; 3(2): 288-296.
Thurman DJ, Beghi E, Begley CE, Berg AT, Buchhalter JR,
Ding D, Hesdorffer DC, Hauser WA, Kazis L, Kobau R,
Kroner B, Labiner D, Liow K, Logroscino G, Medina MT,
Newton CR, Parko K, Paschal A, Preux PM, Sander JW,
Selassie A, Theodore W, Tomson T, Wiebe S, ILAE
Commission on Epidemiology. Standards for epidemiologic
studies and surveillance of epilepsy. Epilepsia. 2011; 52(7):
–26.
World Health Organization. Neurological Disorders: Public
Health Challenges, Geneva, Switzerland. 2006.
Hirak KM, Chandi CK, Sanjay KD, Lakshmikanta G, Bijan
KG. Epilepsy and its Management: A Review. Journal of
Pharma Sci Tech. 2012; 1(2): 20-26.
Simonato M, Brooks-Kayal AR, Engel J Jr, Galanopoulou
AS, Jensen FE, Moshe SL, O’Brien TJ, Pitkanen A, Wilcox
KS, French JA. The challenge and promise of antiepileptic
therapy development in animal models. Lancet Neurol. 2014;
(9): 949–960.
Musa MA, Abdullahi IM, Kamal MJ, Magaji GM.
Phytochemical screening and anticonvulsant studies of ethyl
acetate fraction of Globimetula braunii on laboratory
animals. Asian Pac J Trop Biomed. 2014; 4(4): 285–289.
Hela SI, Megahed HS, Salem SM, Youness ER.
Monotherapy versus polytherapy in epileptic adolescents.
Maced J Med Sci. 2013; 6(2):174-177.
Jaafar KM, Shajarahtunnur J, Norazah B. Antioxidant
Activity of Leaf Extracts of Globimetula braunii (Engler)
van Tiegh Parasitizing on Piliostigma thonningii and Parkia
biglobosa. J. Teknol. 2017; 79: 43–47.
Tripathi AS, Chitra V, Sheikh NW, Mohale DS, and Dewani
AP. Immunomodulatory Activity of the Methanol Extract of
Amorphophallus campanulatus (Araceae) Tuber. Trop J
Pharma Res. 2010; 9(5): 451-454.
Gurib-Fakim A. Medicinal plants: traditions of yesterday and
drugs of tomorrow. Mol asp Med. 2006; 27(1): 1-93.
Rates SMK. Plants as source of drugs. Toxicon. 2001; 39(5):
-613.
Burkill HM. The useful plants of West Tropical Africa, 2nd
ed., vol. 2. The White Friars Press limited, Great Britain.
; 352-364.
Onyekere PF, Enebechi CK, Nnamani DO, PeculiarOnyekere CO, Okonta EO. Phytochemical analysis and
antimicrobial activity of methanol extract of the leaves of
Hippocratea Welwitschii OLIV. (CELASTRACEAE).
African J Pharm Res Develop. 2019; 11(2): 106 – 115.
Swinyard EA, Kupferberg HJ. Antiepileptic drugs: detection,
quantification, and evaluation. Fed Proc. 1985; 44(10):
–2633.
Sayyah M, Valizadeh J, Kamalinejad M. Anticonvulsant
activity of the leaf essential oil of Laurus nobilis against
pentylenetetrazole and maximal electroshock-induced
seizures. Phytomed. 2002; 9: 212-216.
Swinyard EA, Woodhead JH, White HS, Franlin MR.
General principles: Experimental selection, quantification
and evaluation of anticonvulsants. In R. H. Levy, R. H.
Mattson, B. Melrum, J. K. Penry, F. E. Dreifuss (Eds.).
Antiepileptic Drugs (3rd Edition). 1989; 85-102). New York:
Raven press.
Porter RJ, Cereghino JJ, Gladding GD, Hessie BJ,
Kupferberg HJ, Scoville B, White BG. Antiepileptic drug
development program. Cleveland Clin Quart. 1984; 51(2):
-305.
Stanley JL, Rachael JL, Brown TA. McDonald ML, Dawson
RG, Reynolds SD. The mouse beam walking assay offers
improved sensitivity over the mouse rotarod in determining
motor coordination deficits induced by benzodiazepines. J
Psychopharm. 2005; 19(3): 221-227.
Obiloma AA, Ogbonna PC, Okere TO, Adumanya OC,
Maduforo AN. Chemical Composition of Hippocratea
welwitchi: a Common Indigenous Spice Consumed in Southeastern Nigeria. J Diet Assoc Nigeria. 2017; 8: 131-135.
Okwute SK, Okoh-Esene RU, Okogun JI. Two Triterpenoids
Isolated from the Root of Hippocratea welwitschii
(celastraceae)-oliv. UW. Trends in Sci Technol J. 2018; 3(2):
–540.
Illodigwe EE, Akah PA, Okoye TC, Omeje EO.
Anticonvulsant effects of a glycoside isolated from the leaf
of Spathodea campanulata P. Beauv. J Med Res. 2010;
(18): 1894-1900.
Swinyard EA, Woodhead JH. General principles of
detection, quantification and evaluation of anticonvulsants in
Antiepileptic drugs woodbury DM. Penry JK and Pippenger
CE, eds, Raven press. 1982; 113.
White HS, Woodhead JH, Franklin MR, Swinyard EA, Wolf
HH. Experimental selection qualification and evaluation of
antiepileptic drugs 4thedn. Levy LH. Mattson RH and
Meldrum BS. Eds. Raven press, New Work. 1995; 99-100.
Sayyah M, Nadjafnia L, Kamalinejad M. Anticonvulsant
activity and chemical composition of Artemisia dracunculus
L. essential oil. J Ethnopharmacol 2004; 94: 283-287.
Subramaniam S, Rho JM, Penix L, Donevan SD, Fielding
RP, Rogawski MA. Felbamate block of the N-methyl-Daspartate receptor. J Pharmacol Exp Ther 1995; 273(2): 878-
Prigol M, Brüning CA, Godoi B, Nogueira CW, Zeni G. mTrifluoromethyl-diphenyl diselenide attenuates
pentylenetetrazole-induced seizures in mice by inhibiting
GABA uptake in cerebral cortex slices. Pharmacol Rep 2009;
: 1127- 1133.
Czapin ́ski P, Blaszczyk B, Czuczwar SJ. Mechanisms of
action of antiepileptic drugs. Curr Top Med Chem 2005; 5:
-14.
Sun XY, Zhang L, Wei CX, Piao HR, Quan ZS.
Characterization of the anticonvulsant activity of doxepin in
various experimental seizure models in mice. Pharmacol Rep
; 61: 245-251.
Yudkoff M, Daikhin Y, Nissim I, Horyn O, Luhovyy B,
Lazarow A. Short-term fasting, seizure control and brain
amino acid metabolism. Neuro chem Int 2006; 48: 650-656.
Yang J, Wetterstrand C, Jones RS. Felbamate but not
phenytoin or gabapentin reduces glutamate release by
blocking presynaptic NMDA receptors in the entorhinal
cortex. Epilepsy Res 2007; 77(23): 157-164.
Mante PK, Adongo DW, Woode E, Kukuia KKE, Elvis
OforiAmeyaw, EO. Anticonvulsant Effect of Antiaris
toxicaria (Pers.) Lesch. (Moraceae) Aqueous Extract in
Rodents. Hindawi Publishing Corporation ISRN
Pharmacology. 2013; 9.
Bigler ED. “Comparison of effects of bicuculline, strychnine,
and picrotoxin with those of pentylenetetrazole on photically
evoked after discharges,” Epilepsia. 1977; 18(4): 465–470.
Trailovic SM, Varagic VM. The Effect of Ivermectin on
Convulsions in Rats Produced by Lidocaine and Strychnine.
Vet Res Comm. 2007; 31: 863–872
Kehne JH, Kane JM, Miller FP, Ketteler HJ, Braun DL,
Senyah Y, Chaney SF, Abdallah A, Dudley MW, Ogden
AM, Palfreyman MG. MDL 27, 531 selectively reverses
strychnine-induced seizures in mice. BJ. Pharmacol. 1992;
: 910-916.