Anti-SARS-CoV-2 Activity and Acute Toxicity Screening of <i>Annona muricata</i> and <i>Artemisia annua</i> Leaf Extracts

http://www.doi.org/10.26538/tjnpr/v8i1.45

Authors

  • Osayemwenre Erharuyi Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, PMB 1154, Benin City, Nigeria 
  • Sylvester Aghahowa Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, PMB 1154,Benin City, Nigeria 
  • Etinosa Igbinosa Department of Microbiology, Faculty of Life Sciences, University of Benin, PMB 1154,Benin City, Nigeria 
  • Vincent O. Imieje Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, PMB 1154, Benin City, Nigeria 
  • Osahon K. Ogbeide Department of Chemistry, Faculty of Physical Sciences, University of Benin, PMB 1154,Benin City, Nigeria 
  • Isaac U. Akhigbe Department of Chemistry, Faculty of Physical Sciences, University of Benin, PMB 1154,Benin City, Nigeria 
  • Ichoron Nahandoo Department of Chemistry, University of Agriculture PMB 2373, Makurdi, Nigeria
  • Bukola E. Olowoeyo Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, PMB 1154, Benin City, Nigeria 
  • Paschal C. Akubuiro Department of Chemistry, Faculty of Physical Sciences, University of Benin, PMB 1154,Benin City, Nigeria 
  • James Ayorinde Department of Biochemistry and Nutrition, Nigeria Institute of Medical Research, Yaba, Lagos, Nigeria.
  • John O. Igoli Department of Chemistry, University of Agriculture PMB 2373, Makurdi, Nigeria
  • Abiodun Falodun Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, PMB 1154, Benin City, Nigeria 

Keywords:

Toxicity, Antiviral activity, SARS-CoV-2, COVID-19, Phytochemicals, Medicinal plants

Abstract

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Available data suggests that there is no specific treatment in modern medicine. The present study aims to investigate the anti-SARS-CoV-2 activity of Annona muricata and Artemisia annua leaf extracts as well as their acute toxicity study.

The phytochemical screening of the plant extracts was done using standard methods. The acute toxicity evaluation was done using Lorke method. The antiviral activity screening against SARS-CoV-2 was done by plaque assay with Vero-E6 cell line.

Phytochemical screening revealed the presence of alkaloids, terpenoids, flavonoids, saponins and steroids in both plants, while tannins and anthraquinone glycosides were absent. The quantification of SARS-CoV-2 by plaque assay with Vero (E6) cell line shows a reduction in the zones of cellular death for the extracts treated cells compared to the cells with the virus only. Acute toxicity test showed no death or abnormal behavioural changes in mice during and after 24 hours of extract administration.

On the basis of the results obtained from the present study, both Annona muricata and Artemisia annua leaf extracts are considered to have some level of inhibition against SARS-CoV-2 virus growth.

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Published

2024-02-01

How to Cite

Erharuyi, O., Aghahowa, S., Igbinosa, E., Imieje, V. O., Ogbeide, O. K., Akhigbe, I. U., … Falodun, A. (2024). Anti-SARS-CoV-2 Activity and Acute Toxicity Screening of <i>Annona muricata</i> and <i>Artemisia annua</i> Leaf Extracts: http://www.doi.org/10.26538/tjnpr/v8i1.45. Tropical Journal of Natural Product Research (TJNPR), 8(1), 6028–6034. Retrieved from https://tjnpr.org/index.php/home/article/view/3424

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