Antibacterial and Wound Healing Effects of Chitosan-Silver Nanoparticle and Binahong (<i>Anredera cordifolia</i>) Gel Modified with Cinnamon Essential Oil
http://www.doi.org/10.26538/tjnpr/v8i1.32
Keywords:
Wound healing, Antibacterial, Cinnamon essential oil, GelAbstract
Wound healing is a complex biological process that restores the skin structure to its original state following an injury. Improper wound treatment could lead to microbial infection. The extensive use of antibacterial drugs for the treatment of wound infections has encouraged the growth of multidrug-resistant bacteria, leading to the development of serious opportunistic infections. The use of gel formulations containing cinnamon essential oil (CEO) can prevent microbial infection and fascilitates wound healing. This study is aimed at evaluating the antibacterial activity of chitosan (Chit)-silver nanoparticle (AgNp), and binahong leaf extract (BLE) gel modified with CEO against Staphylococcus aureus, and to investigate the gel’s wound healing effect. The gel was formulated with CEO in different concentrations; 9, 7, 5, 3, 1, and 0% (without CEO). The physicochemical properties of the different formulations, including odour, colour, texture, homogeneity, pH, and spreadability. were assessed. The antibacterial and wound healing activities of the gel formulations were investigated in Staphylococcus aureus infected excision wound in Wistar rats. The gel modified with CEO has an average pH ranging from 4.53 to 5.16 and an average spreadability of 5.24 to 5.33 cm. The gel formulations at an optimum concentration of 7% CEO show significant antibacterial activity against Staphylococcus aureus with inhibition zone diameter of 19.5 mm. Histologically, gel formulations with CEO were found to exhibit significant wound healing effect. Overall, the results from the study suggest that gels formulated with CEO have the potential to be used as an alternative treatment for wound infection.
References
Asri H, Adrianto AA, Fadhila N. The effectiveness of topical administration of noni fruit extract ointment with graded concentrations on the number of fibroblasts (research on male Wistar white rats made with incisions). Medicomplement J. 2021; 1(1):7-12.
Susanti PG and Cahyaningrum SE. Characterization and effectiveness test gel of Aloe vera combination chitosan as Staphylococcus aureus antibacterial. UNESA J Chem. 2022; 11(1):26-33.
Mariani F and Galvan EM. Staphylococcus aureus in polymicrobial skinand soft tissue infections: Impact of inter-species interactionsin disease outcome. Antibiotics. 2023; 12(7):1164.
Theerdhala S and Harikrishnan N. Mupirocin loaded niosomal gel for topical wound healing applications. Trop J Nat Prod Res. 2023; 7(8):3676-3682.
Aulia ID and Cahyaningrum SE. Characterization and antibacterial activity test of chitosan-silver nanoparticle gel and binahong leaf extract (Anredera cordifolia (Tenore) Steen) against Staphylococcus aureus. J Pijar Mipa. 2023; 18(4):650-658.
Mirzajani F, Askari H, Hamzelou S, Schober Y, Römpp A, Ghassempour A, Spengler B. Proteomics study of silver nanoparticles toxicity on Oryza sativa L. Ecotoxicol Environ Saf. 2014; 108:335-339.
Hajji S, Khedir SB, Hamza-Mnif I, Hamdi M, Jedidi I, Kallel R, Boufi S, Nasri, M. Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects. Biochim Biophys Acta Gen Subj. 2019; 1863(1):241-254.
Bagheri M, Validi M, Gholipour A, Makvandi P, Sharifi E. Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect. Bioeng Transl Med. 2021; e10254.
Cremar L, Gutierrez J, Martinez J, A Materon L, Gilkerson R, Xu F, Lozano K. Development of antimicrobial chitosan based nanofiber dressings for wound healing applications. Nanomed J. 2018; 5(1):6-14.
Dahm H. Chapter 9, Silver Nanoparticles in Wound Infections: Present Status and Future Prospects. In Nanotech in Skin, Soft Tissue, and Bone Infec. Springer. 2020.
Paladini F and Pollini M. Antimicrobial Silver Nanoparticles for Wound Healing Application: Progress and Future Trends. Materials. 2019; 12(2540):1-16.
Haryani Y, Kartika GF, Yuharmen Y, Putri EM., Alchalish DT, Melanie Y. Utilization of red ginger rhizome water extract (Zingiber officinale Linn. var. rubrum) for simple biosynthesis of silver nanoparticles. Chimica et Natura Acta. 2016; 4(3):151-155.
Inayati I, Arifin NH, Febriansah R, Indarto D, Suryawati B, Hartono H. Trans-cinnamaldehyde inhibitory activity against mrkA, treC, and luxS genes in biofilm-forming Klebsiella pneumoniae: An in silico study. Trop J. Nat Prod Res. 2023; 7(10):4249-4255.
Yuan X, Han L, Fu P, Zeng H, Lv C, Chang W, Runyon RS, Ishii M, Han L, Liu K, Fan T, Zhang W, Liu R. Cinnamaldehyde accelerates wound healing by promoting angiogenesis via up-regulation of PI3K and MAPK signaling pathways. Lab Invest. 2018; 98(6):783-798.
Kim Y, Kim S, Cho KH, Lee JH, Lee J. Antibiofilm activities of cinnamaldehyde analogs against uropathogenic Escherichia coli and Staphylococcus aureus. Int J Mol Sci. 2022; 23(13):7225.
Mousavi F, Bojko B, Bessonneau V, Pawliszyn J. Cinnamaldehyde characterization as an antibacterial agent toward E. coli metabolic profile using 96-blade solid-phase microextraction coupled to liquid chromatography–mass spectrometry. J Proteome Res. 2016; 15(3):963-975.
Firmino DF, Cavalcante TTA, Gomes GA, Firmino NCS, Rosa LD, de Carvalho MG, Catunda FEA Jr. Antibacterial and antibiofilm activities of Cinnamomum sp. essential oil and cinnamaldehyde: Antimicrobial activities. Sci World J. 2018; 2018:7405736.
Pang D, Huang Z, Li Q, Wang E, Liao S, Li E, Zou Y, Wang W. Antibacterial mechanism of cinnamaldehyde: Modulation of biosynthesis of phosphatidylethanolamine and phosphatidylglycerol in Staphylococcus aureus and Escherichia coli. J Agric Food Chem. 2021; 69(45):13628-13636.
Kumalasari ML and Andiarna F. Phytochemical test of ethanol extract of basil leaves (Ocimum basilicum L). Indones J. Heal Sc. 2020; 4(1):39–44.
Rivai H, Misfadhila S, Sari LK. Qualitative and Quantitative Analysis of Chemical Contents of Hexane, Acetone, Ethanol and Water Extracts from Turmeric Rhizomes (Curcuma domestica Val). J Sains Farmasi & Klinis. 2019; March:1-16.
Ariyanta HA. Silver nanoparticles preparation by reduction method and its application as antibacterial for cause of wound infection. J MKMI. 2016; 10(1):36-42.
Ydollahi M, Ahari H, Anvar AA. Antibacterial activity of silver-nanoparticles against Staphylococcus aureus. Afr J Microbiol Res. 2016; 10(23):850–855.
Simanjuntak RCM, Rlyoly W, Fretes FD, Gintu AR. Biofilm chitosan as modern dressing for ulcers. JKPK. 2021; 6(3):335-342.
Magani AK, Tallei TE, Kolondam BJ. Antibacterial test of chitosan nanoparticles against Staphylococcus aureus and Escherichia coli. J Bios Logos. 2020; 10(1):7-12.
Emelda E, Husein S, Saputri D, Yolanda. Formulation and testing of physical properties of single gel preparations and combinations of ethanolic extracts of red betel leaves (Pipper crocatum) and cinnamon oil. Inpharnmed J. 2020; 4(2):43–53.
Alsarra IA. Chitosan topical gel formulation in the management of burn wounds. Int J Biol Macromol. 2009; 45(1):16-21.
Nurhayati LS, Yahdiyani N, Hidayatulloh A. Comparison of the antibacterial activity of yogurt starter with disk diffusion agar and well diffusion agar methods. J Teknologi Hasil Peternakan. 2020; 1(2):41-46.
Lestari NR and Cahyaningrum SE. Synthesis and characterization of hydroxyapatite - nanoperak as anti bacteria that cause dental caries. Indo J Chem Sci. 2022; 11(1):33-40.
Ferro TA, Araújo JM, Dos Santos Pinto BL, Dos Santos JS, Souza EB, da Silva BL, Colares VL, Novais TM, Filho CM, Struve C, Calixto JB, Monteiro-Neto V, da Silva LC, Fernandes ES. Cinnamaldehyde inhibits Staphylococcus aureus virulence factors and protects against infection in a Galleria mellonella model. Front Microbiol. 2016; 21(7):2052
Quazi A, Patwekar M, Patwekar F, Mezni A, Ahmad I, Islam F. Evaluation of wound healing activity (excision wound model) of ointment prepared from infusion extract of polyherbal tea bag formulation in diabetes-induced rats. Evid Based Complement Alternat Med. 2022; 2022:1372199.
Kintoko K, Karimatulhajj H, Elfasyari TY, Ihsan EA, Putra TA, Hariadi P, Ariani C, Nurkhasanah, N. Effect of diabetes condition on topical treatment of binahong leaf fraction in wound healing process. Trad Med J. 2017; 22(2):103-110.
Panjaitan CC, Widyarman AS, Amtha R, Astoeti TE. Antimicrobial and antibiofilm activity of cinnamon (Cinnamomum burmanii) extract on periodontal pathogens-an in vitro study. Eur J Dent. 2022; 16(4):938-946.
Nugroho B and Cahyaningrum SE. Synthesis and characterization of hydroxyapatite-nanosilver-clove oil (Eugenia caryophyllus) as antibacterial in toothpaste preparations against Streptococcus mutans bacteria. J. Pijar Mipa. 2023; 18(4):659–665.
Chenthamara D, Subramaniam S, Ramakrishnan SG, Krishnaswamy S, Essa MM, Lin F, Qoroffleh MW. Therapeutic efficacy of nanoparticles and routes of administration. Biomater Res. 2019; 23(20).
Abdussalam MW, Mohamed L, Abraheem MS, Mansour MMA., Sherif AM. Biofabrication of silver nanoparticles using teucrium apollinis extract: characterization, stability, and their antibacterial activities. Chem. 2023; 5(1):54-64.
Sikorski D, Bauer M, Frączyk J, Draczyński Z. Antibacterial and antifungal properties of modified chitosan nonwovens. Polym. 2022; 14(9):1-17.
Ramalingam B, Khan MR, Mondal B, Mandal AB, Das SK. Facile synthesis of silver nanoparticles decorated magnetic-chitosan microsphere for efficient removal of dyes and microbial contaminants. ACS Sustainable Chem Eng. 2015; 3(9):2291–2302.
Li Y and Kong DWH. Analysis and evaluation of essential oil components of cinnamon barks using GC-MS and FTIR spectroscopy. Ind Crop Prod. 2013; 41:269-278.
Behrooz AB, Fereshteh F, Fahimeh LA, Moones V, Farideh TY. Chemical composition and antioxidant, antimicrobial, and antiproliferative activities of Cinnamomum zeylanicum bark essential oil. Evid-Based Complement Alternat Med. 2020; 2020:1-8.
Muhaimin FI, Cahyaningrum SE, Lawarti RA, Maharani DK. Characterization and antibacterial activity assessment of hydroxyapatite-betel leaf extract formulation against Streptococcus mutans in vitro and in vivo. Indo J Chem. 2023; 23(2):358-369.
Utamia DT, Hertianib T, Pratiwib SU, Haniastuti T, Randy A, Priyanto JA, et al. Correlation analyses of the oral biofilm growth inhibition towards hydrophobicity reduction of oral pathogenic bacteria. Trop J. Nat Prod Res. 2023; 7(10):4141-4145.
Herdyastuti N, Fauziah Widodo R, Prabowo YY, Apriliana IA, Cahyaningrum SE. Diversity of chitinolytic bacteria from shrimp farms and their antifungal activity. J Nat Sci Bio Med. 2021; 12(3):317-324.
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