Antioxidant Activity, Total Phenolic and Total Flavonoid Contents of Etlingera elatior (Jack) R.M. Smith from North Luwu, Indonesia http://www.doi.org/10.26538/tjnpr/v8i1.34
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Abstract
Etlingera elatior (Jack) R.M. Smith is an endemic plant of North Luwu, Indonesia. It is used traditionally as acidifiers in food. This study aim to evaluate the antioxidant activity, total phenolic and total flavonoid contents of the ethanol leaf extract of E. elatior. The dried leaves of E. elatior were extracted by maceration in 70% ethanol at room temperature for three days. The antioxidant activity of the extract was assessed using the 2,2-diphenyl-1-picrilhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays and ferric reducing antioxidant power (FRAP) method. The total phenolic and total flavonoid contents were determined using the Folin-Ciocalteu and Aluminium chloride colorimetric methods. The results showed that E. elatior leaf extract had antioxidant activity with IC50 value of 42.45 ± 1.37 µg/mL compared to 2.78 ± 0.01 µg/mL for vitamin C (Positive control) for DPPH radical scavenging activity. While for the ABTS scavenging activity, the IC50 was 26.46 ± 0.09 µg/mL compared to 0.15 ± 0.02 µg/mL for vitamin C. In the FRAP assay, the extract showed a ferric reducing antioxidant power of 78.52 ± 0.26 mgAAE/g extract. The total phenolic and total flavonoid contents of the extract were 27.19 ± 0,07 mgGAE/g extract and 4,60 ± 0,01 mgQE/g extract, respectively. From the results of the study, it can be concluded that the ethanol leaf extract of E. elatior has strong antioxidant activity which could be attributed to its high phenolic and flavonoid contents.
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References
Badarinath AV, Rao KM, Chetty CMS, Ramkanth S, Rajan TVS, Gnanaprakash K. A Review on In-Vitro Antioxidant Methods: Comparisons, Correlations and Considerations. Int J PharmTech Res. 2010; 2(2):1276-1285.
Sitorus E, Momuat LI, Katja DG. Antioxidant Activity of Seruhan Plant (Peperomia pellucida [L.] Kunth). J Sci. 2013; 13(1):80-85.
Aditya M and Ariyanti PR. Benefits of Gambir (Uncaria gambir Roxb) as an antioxidant. J Major. 2016; 5(3):129-133.
Jabbar A, Wahyuni W, Malaka M, Apriliani A. Antioxidant Activity of Ethanol Extract of Fruit, Leaves, Stems and Rhizomes on Wualae (Etlingera elatior (Jack) R.M Smith) Plants. J Farm Galen. 2019; 5(2):189.
Handayani V and Ahmad AR. Phytochemical Laboratory, Faculty of Pharmacy, Indonesian Muslim University, Makassar; Sudir, M.; Phytochemical Laboratory, Faculty of Pharmacy, Indonesian Muslim University, Makassar. Antioxidant Activity Test of Methanol Extract of Patikala Flowers and Leaves (Etlingera elatior (Jack) R.M.Sm) Using the DPPH Method. Pharm S Res. 2014; 1(2):86–93.
Lachumy SJT, Sasidharan S, Sumathy V, Zuraini Z. Pharmacological Activity, Phytochemical Analysis and Toxicity of Methanol Extract of Etlingera elatior (Torch Ginger) Flowers. Asian Pac J Trop Med. 2010; 3(10):769–774.
Utami YP. Measurement of Simplicia Parameters and Ethanol Extract of Patikala Leaves (Etlingera elatior (Jack) R.M. Sm) from Enrekang Regency, South Sulawesi. J of Pharmacy and Pharmacology Magazine.2020; 24(1):6.
Utami YP, Kristiyanti AMD, Imrawati. Optimization of Distillation Time on the Content and Characteristics of the Essential Oil of Patikala Leaves (Etlingera elatior (Jack) R.M.Smith). J Katalisator 2022; 7(2):205–212.
Utami YP, Ismail I, Nisa M, Oktaviani I. Antibacterial Activity Test of Etlingera elatior (Jack) R.M. Sm Leaf Extract against Bacteria Staphylococcus aureus and Escherichia coli Diffusion Method. Int J Res Pharm Pharm Sci. 2023; 8(3):1–4.
Kumar K, Kamboj R, Rao N, Yadav J. Status and Role of Coccinellids in Insect Pest Management. In: Karem Ghoneim (Ed), Advances in Agricultural Entomology, AkiNik Pub, Vol. 7, 2019. 15–33 p.
Munteanu IG and Apetrei C. Analytical Methods Used In Determining Antioxidant Activity: A Review. Int J Mol Sci. 2021; 22(7):3380.
Hapsari BW, Manikharda, Setyaningsih W. Methodologies in the Analysis of Phenolic Compounds in Roselle (Hibiscus sabdariffa L.): Composition, Biological Activity, and Beneficial Effects on Human Health. Horticult. 2021; 7(2):35.
Yuniarti R, Nadia S, Alamanda A, Zubir M, Syahputra RA, Nizam M. Characterization, Phytochemical Screenings and Antioxidant Activity Test of Kratom Leaf Ethanol Extract (Mitragyna speciosa Korth) Using DPPH Method. J Phy Conf Ser. 2020; 1462:012026.
Sholekah FF. Differences in Altitude on The Content of Flavonoids and Beta Carotene in Karika Fruit (Carica pubescens) Dieng Wonosobo Area. Proc Natl Semin Biol Biol Educ Yogyak. 2017; 75-82.
Susana I, Ridhay A, Bahri S. Study of Antioxidant Activity of Kecombrang Stem Extract (Etlingera elatior) Based on Solvent Polarity Level. J of Covalent Chemistry Research. 2018; 4(1):16–23.
Setiawan F, Yunita O, Kurniawan A. Testing the Antioxidant Activity of Ethanol Extract of Secang Wood (Caesalpinia sappan) Using the DPPH, ABTS, and Frap Methods. J of Science and Health. 2018; 2:82–89.
Serlahwaty D and Sevian AN. Antioxidant Activity Test of 96% Ethanol Extract Combination of Strawberries and Tomatoes Using the ABTS Method. Proceedings of the 50th National Seminar on Indonesian Medicinal Plants. 2016; 3:322–330.
Aminah A, Muflihunna A, Abidin Z. Antioxidant Activity Test of Ethyl Acetate Fraction of Wungu (Graptophyllum pictum (Linn) Griff) Leaves Using the FRAP Method (Ferric Reducing Antioxidant Power). J Ilm -Syifaa. 2016; 8:39–44.
Indrisari M, Aksa R, Khair N, Agiel FMB, Harmawati H, Muslimin L. Acute Safety Assessment of Ethanol Leaves Extract of Cemba (Acacia rugata L.) on The Kidney in Male Wistar Rats. Trop J Nat Prod Res. 2023; 7(7):3360–3363.
Thao TTP, Tu PTC, Men TT. A Comparative Study on Polyphenol, Flavonoid Content, Antioxidant and Antiinflammatory Capacity of Different Solvent Extract from Portulaca oleracea in Carrageenan-Induced Paw Edema in Mice. Trop J Nat Prod Res. 2023; 7(10):4152-4159.
Kamari FE, Chlouchi A, Hamzaoui NE, Harmouzi A, Lhilali I, Amrani Jihane E, El-Mouhdi K, El Omari H, Abdellaoui A. Chemical Composition, Antioxidant and Antimicrobial Activities of The Essential Oil of Origanum majorana Growing in Middle Atlas of Morocco. Trop J Nat Prod Res. 2023; 7(10):4232-4237.
Saralieva EN, Petkova NT, Ivanov Van G, Aneva IY, Georgiev VG, Nikolova KT. Polyphenolic Compounds, Triterpenes, Carlina Oxide, Antioxidant Activity And Carbohydrate Profile Of Different Vegetal Parts Of Carlina vulgaris L., Carlina acanthifolia All. and Carlina corymbosa. Trop J Nat Prod Res. 2023; 7(10):4242-4248.
Hasanah N and Novian DR. Analysis of Ethanol Extract of Yellow Pumpkin (Cucurbita moschata D.).Parapemikir J Ilm Farm. 2020; 9(1):54.
Santoso U. Antioksidan pangan; Ugm Press, 2021.
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang C-M. Determination of Antioxidants By DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Mol Basel Switz. 2022; 27(4):1326.
Ingrid HM and Santoso H. Extraction of Antioxidants and Active Compounds from Kiwi Fruit (Actinidia deliciosa). Unpar J Engr Sci . 2014; 2:43.
Herni K, Anas S, Ajeng D, Iskandar Y. Cytotoxicity of Quercetin and Quercetin-3-O-Rhamnoside of Etlingera elatior (Jack) R.M.Sm. Leaves against HeLa Cervical Cancer Cells. J Appl Pharm Sci. 2021; 11(05):085–090.
Ulfa SM. Department of Chemistry, Faculty of Science and Technology, State Islamic University (Uin) Maulana Malik Ibrahim Malang. 2016.
Maryam S, Baits M, Nadia A. Measurement of the Antioxidant Activity of Ethanol Extract of Moringa Leaves (Moringa oleifera Lam.) Using the FRAP Method (Ferric Reducing Antioxidant Power). J Fitofarmaka Indones. 2016, 2(2):115–118.
Maulana KA, Naid T, Dharmawat DT, Pratama M. Analysis of Antioxidant Activity of Jackfruit Seed Extract (Artocarpus heterophyllus Lam) Using the FRAP (Ferric Reducing Antioxidant Power) Method. J Bionat. 2019; 20(1):27-33.
Maulida Z. Antioxidant Activity of Flavanoid Isolates from the Ethanol Extract of Butterfly Pea Flowers (Clitoria ternatea) with Frap Method. Benzena Pharm Sci J. 12 2023.
Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of Total Flavonoid Content in Propolis By Two Complementary Colometric Methods. J Food Drug Anal. 2002; 10(3): Article 3.
Kupina S, Fields C, Roman MC, Brunelle SL. Determination Of Total Phenolic Content Using The Folin-C Assay: Single-Laboratory Validation, First Action 2017.13. J AOAC Int. 2018, 101(5):1466–1472.
Royani A, Hanafi M, Julistiono H, Manaf A. The Total Phenolic And Flavonoid Contents of Aloe vera and Morinda citrifolia Extracts as Antibacterial Material against Pseudomonas aeruginosa. Mater J Today Proc. 2023, 72:2796–2802.
Da Silva LAL, Pezzini BR, Soares L. Spectrophotometric Determination of The Total Flavonoid Content In Ocimum basilicum L. (Lamiaceae) Leaves. Pharmacogn Mag. 2015; 11(41):96–101.