Effects of Solvent Variation on The Antioxidant, Anti-Inflammatory, and Alpha-Glucosidase Inhibitory Activity of <i>Andrographis paniculata</i> (Burm.f.)Wall Leaves Extract

http://www.doi.org/10.26538/tjnpr/v8i1.36

Authors

  • Candra Irawan Department of Food Nanotechnology Politeknik AKA Bogor, Bogor, Indonesia 16158
  • Riri Enriyani Department of Analytical Chemistry Politeknik AKA Bogor, Bogor, Indonesia 16158
  • Ismail Department of Analytical Chemistry Politeknik AKA Bogor, Bogor, Indonesia 16158
  • Maman Sukiman Department of Industrial Waste Treatment, Politeknik AKA Bogor, 16158, Indonesia
  • Imalia Dwi Putri Department of Food Nanotechnology Politeknik AKA Bogor, Bogor, Indonesia 16158
  • Andita Utami Department of Analytical Chemistry Politeknik AKA Bogor, Bogor, Indonesia 16158
  • Lintannisa Rahmatia Department of Analytical Chemistry Politeknik AKA Bogor, Bogor, Indonesia 16158
  • Anisa Lisandi Department of Analytical Chemistry Politeknik AKA Bogor, Bogor, Indonesia 16158

Keywords:

Andrographis paniculata (Burm.f.) Wall, alpha-glucosidase, anti-inflammatory, antioxidant, Ultrasonic Assist Extraction

Abstract

Andrographis paniculata (Burm.f.) Wall, Acanthaceae, is a plant that grows in the lowlands at 700 meters above sea level. This study aimed to determine the antioxidant activity, alpha-glucosidase enzyme inhibition, and anti-inflammatory activity of Andrographis paniculata (Burm.f.) Wall leaf extract using ethanol and butanol as extraction solvents. The extraction process was accomplished using the Ultrasonic Assist Extraction (UAE) method. The result showed that ethanol and butanol extract yields were 8.88% and 9.02%, respectively. The ethanol and butanol extracts exhibited potent antioxidant activity with IC50 values of (2 – 45) mg/L from the FRAP assay. The extracts also demonstrated potent anti-inflammatory activity with an IC50 range of 0.5-15 mg/L. Also, the ethanol extract showed good alpha-glucosidase inhibitory activity with IC50 = 9.49 ± 0.04 mg/L. The butanol extract showed inhibitory activity against alpha-glucosidase enzyme with an IC50 value of 29.12 ± 0.019 mg/L. The results also showed that the antioxidant, anti-inflammatory, and alpha-glucosidase inhibitory activity of the ethanol leaf extract of Andrographis paniculata (Burm.f.) Wall was slightly higher but not significantly different from butanol; as such, butanol could be used as an alternative solvent to ethanol.

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Published

2024-02-01

How to Cite

Irawan, C., Enriyani, R., Ismail, Sukiman, M., Putri, I. D., Utami, A., … Lisandi, A. (2024). Effects of Solvent Variation on The Antioxidant, Anti-Inflammatory, and Alpha-Glucosidase Inhibitory Activity of <i>Andrographis paniculata</i> (Burm.f.)Wall Leaves Extract: http://www.doi.org/10.26538/tjnpr/v8i1.36. Tropical Journal of Natural Product Research (TJNPR), 8(1), 5968–5972. Retrieved from https://tjnpr.org/index.php/home/article/view/3411