Effects of Solvent Variation on The Antioxidant, Anti-Inflammatory, and Alpha-Glucosidase Inhibitory Activity of Andrographis paniculata (Burm.f.)Wall Leaves Extract http://www.doi.org/10.26538/tjnpr/v8i1.36
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Abstract
Andrographis paniculata (Burm.f.) Wall, Acanthaceae, is a plant that grows in the lowlands at 700 meters above sea level. This study aimed to determine the antioxidant activity, alpha-glucosidase enzyme inhibition, and anti-inflammatory activity of Andrographis paniculata (Burm.f.) Wall leaf extract using ethanol and butanol as extraction solvents. The extraction process was accomplished using the Ultrasonic Assist Extraction (UAE) method. The result showed that ethanol and butanol extract yields were 8.88% and 9.02%, respectively. The ethanol and butanol extracts exhibited potent antioxidant activity with IC50 values of (2 – 45) mg/L from the FRAP assay. The extracts also demonstrated potent anti-inflammatory activity with an IC50 range of 0.5-15 mg/L. Also, the ethanol extract showed good alpha-glucosidase inhibitory activity with IC50 = 9.49 ± 0.04 mg/L. The butanol extract showed inhibitory activity against alpha-glucosidase enzyme with an IC50 value of 29.12 ± 0.019 mg/L. The results also showed that the antioxidant, anti-inflammatory, and alpha-glucosidase inhibitory activity of the ethanol leaf extract of Andrographis paniculata (Burm.f.) Wall was slightly higher but not significantly different from butanol; as such, butanol could be used as an alternative solvent to ethanol.
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