Antioxidant Properties and Mineral Contents of Different Solvent Extracts of Some Medicinal Plants Cultivated in Algeria

http://www.doi.org/10.26538/tjnpr/v8i1.39

Authors

  • Hadjira Guenane Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat 03000, Algeria
  • Maria Rezzoug Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat 03000, Algeria
  • Boulanouar Bakchiche Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat 03000, Algeria
  • Kaddour Cheraif Laboratory of Biological and Agricultural Sciences (LBAS), Amar Telidji University, Laghouat 03000, Algeria
  • Asmaa S. Mohamed Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza 12411, Egypt
  • Maha A.M. El-Shazly Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), Giza 12411, Egypt

Keywords:

Total flavonoid content Mineral content, Total phenolic content, Antioxidant, Algerian plants

Abstract

The current study aims to evaluate the antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) as well as the mineral contents of the aqueous, ethanol, and acetone extracts of three Algerian plants; Haloxylon scoparium, Artemisia campestris L., and Juniperus phoenicea L. The phytochemical screening of the extracts was done using standard methods. The total phenolic and flavonoid contents were evaluated using the Folin-Ciocalteu’s and Aluminium chloride colorimetric methods, respectively. The antioxidant activity was evaluated using the DPPH, ABTS, TAC, and CUPRAC assays. The mineral content was evaluated using ICP-AES. Phytochemical investigation revealed phenols, flavonoids and tannins as the predominant phytochemicals in the plants. The plant extracts showed various degrees of antioxidant activity. The ethanol extract of Juniperus phoenicea exhibited the highest antioxidant activity in both the DPPH and ABTS assays with EC50 values of 39.74 and 13.42 µg/mL, respectively. While the ethanol and acetone extracts of Haloxylon scoparium showed the highest antioxidant activity in the TAC and CUPRAC, respectively with EC50 values of 101.65 and 263 µg/mL, respectively. The TPC of the extracts ranged from 2.38 to 58.03 mg GAE/g DW, while the TFC ranged from 1.03 to 47.59 mg RE/g DW. The mineral content analysis reveals the presence of vital elements like Ca, Mg, Sr, Mn and Ni in the plants. These plants are considered promising natural sources of antioxidants and nutritional supplements that can be utilized in pharmaceutical formulations.

References

Bakchiche B, Gherib A, Bronze MR, Ghareeb MA. Identification, quantification, and antioxidant activity of hydroalcoholic extract of Artemisia campestris from Algeria. Turk J Pharm Sci. 2019; 16(2):234-239.

Cheraif K, Bakchiche B, Gherib A, Bardaweel SK, Ayvaz MC, Flamini G, Ascrizzi R, Ghareeb MA. Chemical composition, antioxidant, anti-tyrosinase, anti-cholinesterase and cytotoxic activities of essential oils of six Algerian plants. Molecules. 2020; 25(7):1710.

Kemal ME, Bakchiche B, Kemal M, Cheraif K, Kara Y, Bardaweel SK, Miguel MG, Yildiz O, Ghareeb MA. Six Algerian plants: Phenolic profile, antioxidant, antimicrobial activities associated with different simulated gastrointestinal digestion phases and antiproliferative properties. J Herb Med. 2023; 38:100636.

Lamchouri F, Benali T, Bennani B, Toufik H, Hassania LIM, Bouachrine M, Lyoussi B. Preliminary phytochemical and antimicrobial investigations of extracts of Haloxylon scoparium. J Mater Environ Sci. 2012; 3(4):754-759.

Bouaziz A, Mhalla D, Zouari I, Jlaiel L, Tounsi S, Jarraya R, Trigui M. Antibacterial and antioxidant activities of Hammada scoparia extracts and its major purified alkaloids. S Afr J Bot. 2016; 105:89-96.

Fatehi N, Allaoui M, Berbaoui H, Cheriti A, Boulenouar N, Belboukhari N. Haloxylon scoparium: An ethnopharmacological survey, phytochemical screening and antibacterial activity against human pathogens causing nosocomial infection. PhytoChem BioSub J. 2017; 11(2):104-109.

Okolie NP, Falodun A, Davids O. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and its potential for the inhibition of lipid peroxidation. Afr J Trad Complement Alternat Med. 2014; 11(3):221-227.

Ghareeb MA, Mohamed T, Saad AM, Refahy LA, Sobeh M, Wink M. HPLC-DAD-ESI-MS/MS analysis of fruits from Firmiana simplex (L.) and evaluation of their antioxidant and antigenotoxic properties. J Pharm Pharmacol. 2018a; 70:133-142.

Ghareeb MA, Saad AM, Ahmed WS, Refahy LA, Nasr MS. HPLC-DAD-ESI-MS/MS characterization of bioactive secondary metabolites from Strelitzia nicolai leaf extracts and their antioxidant and anticancer activities in vitro. Pharmacogn Res. 2018c; 10:368.

Ghareeb MA, Sobeh M, Rezq S, El-Shazly A, Mahmoud M, Wink M. HPLC-ESI-MS/MS profiling of polyphenolics of a leaf extract from Alpinia zerumbet (Zingiberaceae) and its anti-Inflammatory, anti-nociceptive, and antipyretic activities in vivo. Molecules. 2018b; 23(12):3238.

Sobeh M, Mahmoud MF, Hasan RA, Abdelfattah MAO, Sabry OM, Ghareeb MA, El-Shazly AM, Wink M. Tannin-rich extracts from Lannea stuhlmannii and Lannea humilis (Anacardiaceae) exhibit hepatoprotective activities in vivo via enhancement of the anti-apoptotic protein Bcl-2. Sci Rep. 2018; 8:9343.

Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, Falodun A. Antioxidant and hypoglycaemic potentials of the leaf extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.

Abdelfattah MAO, Dmirieh M, Ben Bakrim W, Mouhtady O, Ghareeb MA, Wink M, Sobeh M. Antioxidant and anti-aging effects of Warburgia salutaris bark aqueous extract: Evidences from in silico, in vitro and in vivo studies. J Ethnopharmacol. 2022; 292:115187.

Fouad MS, Ghareeb MA, Hamed AA, Aidy EA, Tabudravu J, Sayed AM, Tammam MA, Mwaheb MA. Exploring the antioxidant, anticancer and antimicrobial potential of Amaranthus viridis L. collected from Fayoum depression: Phytochemical, and biological aspects. S Afr J Bot. 2024; 166:297-310.

Ibrahim AM and Ghareeb MA.. Preliminary phytochemical screening, total phenolic content, in vitro antioxidant and molluscicidal activities of the methanolic extract of five medicinal plants on Biomphalaria alexandrina snails. J Herbs Spices Med Plants. 2020; 26(1):40-48.

Habib MR, Hamed AA, Ali REM, Zayed KM, Gad El-Karim RM, Sabour R, Abu El-Einin HM, Ghareeb MA. Thais savignyi tissue extract: bioactivity, chemical composition, and molecular docking. Pharm Biol. 2022; 60(1):1899-1914.

Ghareeb MA, Sobeh M, Aboushousha T, Esmat M, Mohammed HS, El-Wakil ES. Polyphenolic profile of Herniaria hemistemon aerial parts extract and assessment of its anti-cryptosporidiosis in a murine model: In silico supported in vivo study. Pharmaceut. 2023; 15:415.

Boulanouar B, Abdelaziz G, Aazza S, Gago C, Miguel M.G. Antioxidant activities of eight Algerian plant extracts and two essential oils. Ind Crops Prod. 2013; 46:85-96.

Rezzoug M, Bakchiche B, Gherib A, Roberta A, Kilinçarslan Ö, Mammadov R, Bardaweel SK. Chemical composition and bioactivity of essential oils and ethanolic extracts of Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut. from the Algerian Saharan Atlas. BMC Complement Alternat Med. 2019; 19:1-10.

Apak R, Güçlü K, Özyürek M, Çelik SE. Mechanism of antioxidant capacity assays and the CUPRAC (Cupric Ion Reducing Antioxidant Capacity) assay. Microchim Acta. 2008; 160:413-419.

Bakchiche B, Gören AC, Aydoğmuş Z, Mataraci-Kara E, Ghareeb MA. Artemisia campestris and Artemisia herba-alba: LC-HRESI-MS profile alongside their antioxidant and antimicrobial evaluation. Acta Pharm Sin. 2022; 60(2):131-152.

Haida S, Kribii A, Daoud NA, Belakhmima RA, Kribii A. Antioxidant activity of Haloxylon scoparium alkaloid extracts from Figuig region (southeastern of Morocco). Braz J Pharm Sci. 2022; 58:e19494.

Elmhdwi MF, Attitalla IH, Khan BA. Evaluation of antibacterial activity and antioxidant potential of different extracts from the leaves of Juniperus phoenicea. J Plant Pathol Microb. 2015; 6: 300.

ZahnitW, Smara O, Bechki L, Bensouici C, Messaoudi M, Benchikha N, Larkem I, Awuchi CG, Sawicka B, Simal-Gandara J. Phytochemical profiling, mineral elements, and biological activities of Artemisia campestris L. grown in Algeria. Horticult. 2022; 8:914

Published

2024-02-01

How to Cite

Guenane, H., Rezzoug, M., Bakchiche, B., Cheraif, K., Mohamed, A. S., & El-Shazly, M. A. (2024). Antioxidant Properties and Mineral Contents of Different Solvent Extracts of Some Medicinal Plants Cultivated in Algeria: http://www.doi.org/10.26538/tjnpr/v8i1.39. Tropical Journal of Natural Product Research (TJNPR), 8(1), 5987–5991. Retrieved from https://tjnpr.org/index.php/home/article/view/3408