Chemical Composition and Antifungal Activity of Essential Oils of <i>Rosmarinus officinalis</i> L. and <i>Salvia officinalis</i> L. against <i>Botrytis cinerea Pers</i>


  • Manal Kasmi Department of biology, Facultyof Sciences and Techniques, Abdelmalek-Essaadi University, Tangier, Morocco
  • Adama Diakite Department of biology, Facultyof Sciences and Techniques, Abdelmalek-Essaadi University, Tangier, Morocco
  • Said Barrijal Department of biology, Facultyof Sciences and Techniques, Abdelmalek-Essaadi University, Tangier, Morocco
  • Haiat Essalmani Department of biology, Facultyof Sciences and Techniques, Abdelmalek-Essaadi University, Tangier, Morocco


Salvia officinalis L., Rosmarinus officinalis L., Essential oil, Chemical composition, Botrytis cinerea Pers, Antifungal effect


Botrytis cinerea Pers. the causative agent of grey mould is commonly controlled by synthetic fungicides. Recently, studies have focused on the use of aromatic and medicinal plant extracts as safer alternatives. The aim of this study was to investigate the chemical composition and the antifungalactivity of essential oils of Rosmarinus officinalis L.(REO) and Salvia officinalis L.(SEO), against B. cinerea Pers.on tomato leaves and fruits. The EOs were extracted by hydrodistillation, and their chemical composition was analysed by gas chromatography-mass spectrometry (GC-MS). The antifungal activity of the EOs was evaluated on tomato leaves and fruitsinfested with B. cinerea Pers. using four treatment methods; simultaneous, preventive, curative, and combined preventive and curative treatments. GC-MS analysis showed that REO is composed mostly of α-pinene (29.01%), verbenone (21.59%), and camphor (7.32%), while SEO is predominantly composed of trans-thujone (29.01%), 1,8-cineole (22.78%), camphor (20.31%), and α-pinene (7.49%). REO and SEO demonstrated a concentration-dependent antifungal activity against B. cinerea Pers, especially in the combined preventive and curative treatment which exhibited 100% fungal growth inhibition for both EOs on tomatoleaves and fruits. On the tomato leaves, REO showed the least antifungal activity in the simultaneous treatment (IC50= 7.62 ± 0.83µL/mL), while on tomato fruits, the curative treatment withSEOexhibited the least effective activity (IC50= 9.15± 0.53µL/mL). Therefore, the essential oils of R. officinalis and S. officinalis. have potential for use in the biocontrol of tomato grey mould under green house and postharvest storage.

Author Biography

Manal Kasmi, Department of biology, Facultyof Sciences and Techniques, Abdelmalek-Essaadi University, Tangier, Morocco

Department of biology, Faculty of Sciences, Mohammed V University, Rabat, Morocco.


FillingerS and Elad Y. Botrytis – the Fungus, the Pathogen and Its Management in Agricultural Systems. Springer International Publishing., Switzerland. 2016.

Elad Y. Effect of filtration of solar light on the production of conidia by field isolates of Botrytis cinerea and on several diseases of green house‐grown vegetables. Crop Prot. 1997; 16(7): 635-642

Dulger B and Hacioglu N. Antifungal activity of Endemic Salvia tigrina in Turkey. Trop J Pharm Res. 2008; 7:1051-1054.

Abdallah RAB, Jabnoun-Khiareddine H, Nefzi A, Mokni-Tlili S, Daami-Remadi M. Endophytic Bacteria from Datura stamonium for Fusarium wilt suppression and tomato growth promotion. J Microb Biochem Sci Technol. 2016; 26(8):1-51.

Fiume F and Fiume F. Biological control of Botrytis cinerea gray mould on tomato cultivated in greenhouse. Comm Appl Biol Sci. 2006; 71:897.

Wang K, Xion Q, Kan Jianquan K, Cao S, Zheng Y. Induction of director priming resistance against Botrytis cinerea in strawberries by β-aminobutyric acid and their effects on sucrose metabolism. J Agric Food Chem. 2016; 64:5855-5865.

Yan D, Zhang Y, Liu L, Yan H. Pesticide exposure and risk of Alzheimer’s disease: A systematic review and meta-analysis. Sci Rep. 2016; 6:1-9.

Cimino AM, Boyles AL, Thayer KA, Perry MJ. Effects of neonicotinoid pesticide exposure on human health: A systematic review. Environ Health Perspect. 2017; 125(2):155-162.

Sun C, Lin M, Fu D, Yang J, Huang Y, Zheng X, Yu T. Yeast cell wall induces disease resistance against Penicillium expansum in pear fruit and the possible mechanisms involved. Food Chem. 2017; 241:301-307.

Brandhorst TT and Klein BS. Uncertainty surrounding the mechanism and safety of the postharvest fungicide fludioxonil. Food Chem Toxicol. 2018; 123:561-565.

Catto C, de Vincenti L, Borgonovo G, Bassoli A, Marai S, Villa F, Cappitelli F, Saracchi M. Sub-lethal concentrations of Perilla frutescens essential oils affect phytopathogenic fungal biofilms. J Environ Manag. 2019; 245:264-272.

Ali A, Bordoh PK, Singh A, Siddiqui Y, Droby S. Post-harvest development of anthracnose in pepper (Capsicum spp): Etiology and management strategies. Crop Protect. 2016; 90:132-141.

Mari M, Bautista-Banos S, Sivakumar D. Decay control in the postharvest system: Role of microbial and plant volatile organic compounds. Postharv Biol Technol. 2016; 122:70-81.

Munir M, Amsden B, Dixon E, Vaillancourt L, Ward Gauthier NA. Characterization of Colletotrichum species causing bitter rot of apple in Kentucky orchards. Plant Dis. 2016; 100(11):2194-2203.

Gonzalez-Estrada RR, Chalier P, Ragazzo-Sanchez JA, Konuk D, Calderon-Santoyo M. Antimicrobial soy protein based coatings: Application to Persian lime (Citrus latifolia Tanaka) for protection and preservation. Postharv Biol Technol. 2017; 132:138-144.

Zhang L, Song L, Xu X, Zou X, Duan K, Gao Q. Characterization and Fungicide Sensitivity of Colletotrichum Species Causing Strawberry Anthracnose in Eastern China. Plant Dis. 2020, Article PDIS-10-19-2241.

Sarkhosh A, Schaffer B, Vargas AI, Palmateer AJ, Lopez P, Soleymani A, Farzaneh M. Antifungal activity of five plant-extracted essential oils against anthracnose in papaya fruit. Biol Agric Horticult. 2017; 34(1):18–26.

Leroch M, Kretschmer M, Hahn M. Fungicide resistance phenotypes of Botrytis cinerea isolates from commercial vineyards in South West Germany. J Phytopathol. 2011; 159:63–65.

Leroch M, Plesken C, Weber RWS, Kauff F, Scalliet G, Hahn M. Gray mold populations in German strawberry fields show multiple fungicide resistance and are dominated by a novel clade close to Botrytis cinerea. Appl Environ Microbiol. 2013; 79:159-167.

Grabke J, Fernandez-Ortuno A, Amiri D, Li A, Peres X, Smith NA, Schnabel P. Characterization of iprodione resistance in Botrytis cinerea from strawberry and blackberry. Phytopathol. 2014; 104:396-402.

Lopes UP, Zambolim L, Capobiango NP, Gracia NAO, Freitas-Lopes RL. Resistance of Botrytis cinerea to fungicides controlling gray mold on strawberry in Brazil. Bragantia. 2017; 76(2):266-272.

Avenot HF, QuattriniJ, Puckett R, Michailides TJ. Different levels of resistance to cyprodinil and iprodione and lack of fludioxonil resistance in Botrytis cinerea isolates collected from pistachio, grape and pomegranate fields in California. Crop Prot. 2018; 112:274-281.

El Ouadi Y, Manssouri M, Bouyanzer A, Majidi L, Lahhit N, Bendaif H, Costa J, Chetouani A, Elmsellem H, Hammouti B. Essential oil composition and antifungal activity of Salvia officinalis originating from North-East Morocco, against postharvest phytopathogenic fungi in apples. Der Pharm Chem. 2015; 7(9):95e102.

González-Tejero MR, Casares-Porcel M, Sánchez-Rojas CP, Ramiro-Gutiérrez JM, Molero-Mesa A, Pieroni D. Paraskeva-Hadijchambi. J Ethnopharmacol. 2008; 116:341-357.

Jamila F and Mostafa E. Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. J Ethnopharmacol. 2014; 154:76- 87.

Ali B, Al-wabel NA, ShamsS, Ahamad A, Khan SA, Anwar F.Essential oils used in aromatherapy: A systemic review. Asian Pac J Trop Biomed. 2015; 5:601-611.

Issouffou C, Suwansri S, Salaipeth L, Domig KJ, Hwanhlem N. Synergistic effect of essential oils and enterocin KT2W2G on the growth of spoilage microorganisms isolated from spoiled banana peel. Food Contr. 2018; 89:260-269.

Oliveira J, Parisi MCM, Baggio JS, Silva PPM, Paviani B, Spoto MHF, Gloria EM. Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose. Int J Food Microbiol. 2019; 292:150-158.

Oliveira J, Gloria EM, Parisi MCM, Baggio JS, Silva PPM, Ambrosio CMS, Spoto MHF. Antifungal activity of essential oils associated with carboxymethylcellulose against Colletotrichum acutatum in strawberries. Sci Horticult. 2019a; 243:261-267.

OuYang Q, Okwong RO, Chen Y, Tao N. Synergistic activity of cinnamaldehyde and citronellal against green mold in citrus fruit. Postharv Biol Technol. 2020; 162:111095.

Zhang X, Guo Y, Guo L, Jiang H, Ji Q. In vitro evaluation of antioxidant and antimicrobial activities of Melaleuca alternifolia essential oil. BioMed Res Int. 2018; 2018:2396109.

Feng G, Zhang XS, Zhang ZK, Ye HC, Liu YQ, Yang GZ, Chen C, Chen M, Yan C, Wang LY, Zhang JX, Zhang J. Fungicidal activities of camptothecin semisynthetic derivatives against Colletotrichum gloeosporioidesin vitroand in mango fruit. Postharv Biol Technol. 2019; 147:139-147.

Jahani M, Pira M, Aminifard MH. Antifungal effects of essential oils against Aspergillus nigerin vitro and in vivo on pomegranate (Punica granatum) fruits. Sci Horticult. 2020; 264:109188.

Matos Ferreira L, Douglas Rafael e Silva B, Emanuel da Cruz L, Dutra KA, Daniela Maria do Amaral Ferraz N, Jessica Lafaiete Ribeiro A, Gutierres Nelson S. Chemical composition and insecticidal effect of essential oils from Illicium verum and Eugenia caryophyllus on Callosobruchus maculatus in cowpea. Ind Crops Prod. 2020; 145:112088.

Mehdizadeh L, Taheri P, Ghasemi PA, Moghaddam M. Phytotoxicity and antifungal properties of the essential oil from the Juniperus polycarpos var. turcomanica (B. Fedsch.) R.P. Adams leaves. Physiol Mol Biol Plants. 2020; 26(4):759-771.

Raspo MA, Vignola MB, Andreatta AE, Juliani HR. Antioxidant and antimicrobial activities of citrus essential oils from Argentina and the United States. Food Biosci. 2020l; 36:100651.

Asgar E, Masumeh Z, Franco P. Molecules. 2020; 25(7):1-15.

Chavez-Gonzalez ML, Rodriguez R, Aguilar CN. Essential Oils: A Natural Alternative to Combat Antibiotics Resistance. In: Kateryna Kon; Mahendra Rai (Eds), Antibiotic Resistance. Mechanisms and New Antimicrobial Approaches, Chapter 11; Elsevier, 2016; 227-237 p.

Zatla AT, Dib MA, Djabou N, Ilias F, Costa J, Muselli A. Antifungal activities of essential oils and hydrosol extracts of Daucus carota subsp sativus for the control of fungal pathogens, in particular gray rot of strawberry during storage. J Essent Oil Res. 2017; 29:391-399.

Mouria A, Ouazzani-Touhami A, Douira A. Mise en évidence d‟une variation intra spécifique chez Botrytis cinerea et lutte biologique in vitro par l‟extrait de compost. J Appl Biosci. 2013; 64:4797-4812.

Kasmi M and Essalmani H.Antifungal Effect of Rosmarinus officinalisL. andSalvia officinalis L. Extracts against Tomato Grey Mould.Trop J Nat Prod Res, February 2022, 6(2), 180-187.

Djeddi S, Bouchenah N, Settar I, Skaltsa HD. Composition and antimicrobial activity of the essential oil of Rosmarinus officinalisfrom Algeria. Chem Nat Comp. 2007; 43(4):487-490.

Rasooli I. Antimycotoxigenic characteristics of Rosmarinus officinalisand Trachyspermum copticum L. essential oils; Int J Food Microbiol. 2008; 122:135-139.

Hussain AI, Anwar F, Iqbal T, Bhatti IA. Antioxidant attributes of fourlamiaceae essential oils. Pak J Bot. 2011; 43(2):1315-1321.

Jiang Y, Wu N, Fu YJ, Wang W, Luo M, Zhao CJ, Zu YG, Liu XL. Chemical composition and antimicrobial activity of the essential oil of Rosemary. Environ Toxicol Pharmacol. 2011; 32(1):63-68.

Elamrani A, Zrira S, Benjilali B. A study of Moroccan rosemary oils. J Ess Oil Res. 2000; 12:487-495.

Flamini G, Cioni PL, Morelli I, Macchia M, Ceccarini L. Main agronomicproductive characteristics of two ecotypes of Rosmarinus officinalisL. and chemical composition of their essential oils. J Agric Food Chem. 2002; 50:3512-3517.

Ainane A, Benhima R, Khammour F, Elkouali M, Talbi M, Abba E, Cherroud S, Ainane T. Composition chimique et activité insecticide de cinq huiles essentielles: Cedrus atlantica, Citrus limonum, Eucalyptus globules, Rosmarinus officinaliset Syzygium aromaticum. BIOSUNE’1. 2018; 67-79 p.

Akbari J, Saeedi M, Farzin D, Morteza-Semnani K, Esmaili Z.Transdermal absorption enhancing effect of the essential oil of Rosmarinus officinalison percutaneous absorption of Na diclofenac from topical gel.Pharm Biol. 2015; 53:1442-1447.

Tak JH, Jovel E, Isman MB.Comparative and synergistic activity of Rosmarinus officinalisL. essential oil constituents against the larvae and an ovarian cell line of the cabbage looper, Trichoplusia ni (Lepidoptera: Noctuidae).Pest Manag Sci. 2016; 72:474-480.

Mekonnen A, Yitayew B, Tesema A, Taddese S. "In Vitro Antimicrobial Activity of Essential Oil of Thymus schimperi, Matricaria chamomilla, Eucalyptus globulus, and Rosmarinus officinalis". Int J Microbiol. 2016; 2016:Article ID 9545693. 8 pages.

Rus CF, Alexa E, Pop G, Sumalan RM, Copolovici M. Antifungal activity and chemical composition of Salvia officinalis L. essential oil. Res J Agric Sci. 2015; 47:186-194.

Radulescu V, Chiliment S, Oprea E. Capillary gas chromatography-mass spectrometry of volatile and semi-volatile compounds of Salvia officinalis. J Chromatogr A. 2004; 1027)1-2):121-126.

Vukovic-Gacic B, Nikcevic S, Beric-Bjedov T, Knezevic-Vukcevic J, Simic D. Antimutagenic effect of essential oil of sage (Salvia officinalis L.) and its monoterpenes against UV-induced mutations in Escherichia coli and Saccharomyces cerevisiae. Food Chem Toxicol. 2006; 44:1730-1738.

Pierozan MK, Pauletti GF, Rota L, Santos ACA, Lerin L, Di Luccio M, Mossi AJ, Atti-Serafini L, Cansian RL, Oliveira JV. Chemical characterization and antimicrobial activity of essential oils of Salvia L. species. Ciência Tecnol Alimen. 2009; 29(4):764-770.

Bouaziz M, Yangui T, Sayadi S. Disinfectant properties of essential oils from Salvia officinalis L. cultivated in Tunisia. Food Chem Toxicol. 2009; 47(11):2755-2760.

Alexa E, Sumalan RM, Danciu C, Obistioiu D, Negrea M, Poiana MA, Rus C, Radulov I, Pop G, Dehelean C. Synergistic Antifungal, Allelopatic and Anti-Proliferative Potential of Salvia officinalis L., and Thymus vulgaris L. Essential Oils. Molecules. 2018; 23:185.

Schmidt E, Wanner J, Hiiferl M, Jirovetz L, Buchbauer G, Gochev V, Girova T, Stoyanova A, Geissler M. Chemical composition, olfactory analysis and antibacterial activity of Thymus vulgaris chemotypes geraniol, 4-thujanol/terpinen-4-ol, thymol and linalool cultivated in southern France. Nat Prod Commun. 2012; 7:1095-1098.

Rguez S, Daami-remadi M, Cheib I, Laarif A, Hamrouni I. Composition Chimique, Activité Antifongique et Activité Insecticide de l’Huile Essentielle de Salvia officinalis. Tunis J Med Plants Nat Prod. 2013; 9(2):65-76.

Adrar N, Oukil N, Bedjou F. Antioxidant and antibacterial activities of Thymus numidicus and Salvia officinalis essential oils alone or in combination. Ind Crops Prod. 2016; 88:112-119.

Santos-Gomes PC and Fernandes-Ferreira M. Organ- and season-dependent variation in the essential oil composition of Salvia officinalis L. cultivated at two different sites. J Agric Food Chem. 2001; 49:2908-2916.

Baranauskiene R, Venskutonis PR, Viškelis P, Dambrauskiene E. Influence of nitrogen fertilizers on the yield and composition of Thyme (Thymus vulgaris). J Agric Food Chem. 2003; 51:7751-7758.

Vianna TC, Marinho CO, Júnior LM, Ibrahim SA, Vieira RP. Essential oils as additives in active starch-based food packaging films: A review. Int J Biol Macromol. 2021; 182(1):1803–1819.

Vintila I. Basic Structure, Nomenclature, Classification and Properties of Organic Compounds of Essential Oil. In: Seyed Hashemi, M.B. (Ed.), Essential Oils in Food Processing: Chemistry, Safety and Applications. First Edition. John Wiley & Sons Ltd. Chap. 5. 2018.

Fernando P, Monteiro I, Larissa C, Ferreira I, Jhonata L, Silva I, Leandro P, Pacheco E, Paulo E. Influence of Plant extracts and essential Oils against Panama Disease (Fusarium oxysporum f. sp. cubense) in Banana Seedlings. J Agric Sci. 2013; 5:4–14.

Buckle, J Clin Aromather. 2015; 37-72.

Freiesleben S and Jager AK. Correlation between Plant Secondary Metabolites and Their Antifungal Mechanisms–A Review. Med Arom Plants. 2014; 03(02):1–6.

Gutierrez-del-Río I, Fernandez J, Lombo F. Plant nutraceuticals as antimicrobial agents in food preservation: Terpenoids, polyphenols and thiols. Int J Antimicrob Agents. 2018; 52(3):309–315.

Tariq S, Wani S, Rasool W, Shafi K, Bhat MA, Prabhakar A, Shalla AH, Rather MA. A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microb Pathogen. 2019; 134:103580.

Fernandes RV, Borges SV, Botrel DA.Gum arabic/starch/maltodextrin/inulin as wall materials on the microencapsulation of rosemary essential oil.Carbohydr Polym. 2014; 101:524-532.

Eugénia P, Salgueiro L, Cavaleiro C, Pameira A, Conçalves M. In vitro susceptibility of certain species of yeast and filamentous fungi with essential oil. Ind Crops Prod. 2007; 26:135- 141.

Marei GIK and Abdelgaleil SAM. Antifungal Potential and Biochemical Effects of Monoterpenes and Phenylpropenes on Plant Pathogenic Fungi. Plant Prot Sci. 2018; 54:9–16.

Zillo RR, da Silva PPM, de Oliveira J, da Gloria EM, Spoto MHF. Carboxymethylcellulose coating associated with essential oil can increase papaya shelf life. Sci Horticult. 2018; 239:70–77.

Wang H, Yang Z, Ying G, Yang M, Nian Y, Wei F, Kong W. Antifungal evaluation of plant essential oils and their major components against toxigenic fungi. Ind Crops Prod. 2018; 120:180–186.

Xing C, Qin C, Li X, Zhang F, Linhardt RJ, Sun P, Zhang A. Chemical composition and biological activities of essential oil isolated by HS-SPME and UAHD from fruits of bergamot. Lwt-Food Sci Technol. 2019; 104:38–44.

Mello AM, Gomes RT, Lara SR, Gomes Silva L, Alves JB, Cort´es ME, Abreu SL, Santos VR. The effect of brazilian propolis on the germ tube formation and cell wall of Candida albicans. Pharmacol Online. 2006; 3:352–358.

Viriato A. Terpenoids with antifungal activity for Candida Berkhout, causing nosocomial infections. World Health. 2014; 38:40–50.

Hossain F, Follett P, Dang Vu K, Harich M, Salmieri S, Lacroix M. Evidence for synergistic activity of plant-derived essential oils against fungal pathogens of food. Food Microbiol. 2016; 53:24–30.

Nikkhah M and Hashemi M. Boosting antifungal effect of essential oils using combination approach as an efficient strategy to control postharvest spoilage and preserving the jujube fruit quality. Postharv Biol Technol. 2020; 164:111159.

Daferera DJ, Ziogas BN, Polissiou MG. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Prot. 2003; 22:39–44.

Sumalan RM, Alexa E, Poiana MA. Assessment of inhibitory potential of essential oils on natural mycoflora and Fusarium mycotoxins production in wheat. Chem Cent J. 2013; 7:32.

Rguez S, Msaada K, Daami-Remadi M, Chayeb I, Rebey IB, Hammami M, Laarif A, Hamrouni-Sellami I. Chemical composition and biological activities of essential oils of Salvia officinalis aerial parts as affected by diurnal variations. Plant Biosyst. - Int J Deal With All Asp Plant Biol. 2018; 1126:1724–5575.

Stangarlin JR and Pascholati SF. Activities of ribulose-1,5-bisphosphate Carboxylaseoxygenase (rubisco), chlorophyllase, β-1,3 glucanase and Chitinase and Chlorophyll Content in Bean Cultivars (Phaseolusvulgaris) infected with Uromycesappendiculatus. Sum. Phytopathol. 2000; 26 (1):34–42.

Lucas GC, Alves E, Pereira RB, Perina FJ, Magela de Sauza R. Antibacterial activity of essential oils on Xanthomonas vesicatoria and control of bacterial spot in tomato. Pesq AgropecBras. 2012; 47(3):351-359.

Banani H, Olivieri L, Santoro K, Garibaldi A, Gullino ML, Spadaro D. Thyme and savory essential oil efficacy and induction of resistance against Botrytis cinerea through priming of defense responses in apple. Foods. 2018; 7(2):11.

Hu Y, Zhang J, Kong W, Zhao G, Yang M. Mechanisms of antifungal and anti-aflatoxigenic properties of essential oil derived from turmeric (Curcuma longa L.) on Aspergillus flavus. Food Chem. 2017; 220:1–8.

Raveau R, Fontaine J, Sahraoui LA. Essential oils as potential alternative biocontrol products against plant pathogens and weeds: A review. Foods. 2020; 9(365):31.

Lagrouh F, Dakka N, Bakri Y. The antifungal activity of Moroccan plants and the mechanism of action of secondary metabolites from plants. J Mycol Med. 2017; 27(3):303–311.

Nazzaro F, Fratianni F, Coppola R, De Feo V. Essential oils and antifungal activity. Pharm. 2017; 10(86):1–20.

Grande-Tovar CD, Chaves-Lopez C, Serio A, Rossi C, Paparella A. Chitosan coatings enriched with essential oils: Effects on fungi involve in fruit decay and mechanisms of action. Trends Food Sci Technol. 2018; 78:61–71.

Caccioni RLD and Guizzardi M. Inhibition of germination and growth of fruit and vegetable postharvest pathogenic fungi by essential oil components. J Essential Oil Res. 2011; 6(2):173–179.

Rguez S, Ben Slimene I, Abid G, Hammemi M, Kefid A, Elkahoui S, Ksouri R, Hamrouni Sellami I, Djébali N. Tetraclinis articulata essential oil reduces Botrytis cinerea infections on tomato. Sci Horticul. 2020; 266:109291.

Bevilacqua A, Speranza B, Perricone M, Sinigaglia M, Corbo MR. Bioactivity of essential oils towards fungi and bacteria: mode of action and mathematical tools. Essential Oils in Food Proc: Chem Saf Applic. 2017; 231–246.

He J, Wu D, Zhang Q, Chen H, Li H, Han Q, Lai X, Wang H, Wu Y, Yuan J, Dong H, Qin W. Efficacy and mechanism of cinnamon essential oil on inhibition of Colletotrichum acutatum isolated from “Hongyang” kiwifruit. Front Microbiol. 2018: 9:1–12.

Sharma N and Tripathi A. Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens. World J Microbiol Biotechnol. 2006: 22:587–593.

PaulaP, Moreira S, Jacqueline O, Anaíle dos Mares B, Marise MP, Eduardo Micoti G, Marta HFS.Essential oils from Eucalyptus staigeriana F. Muell. ex Bailey and Eucalyptus urograndis W. Hill ex Maiden associated to carboxymethylcellulose coating for the control of Botrytis cinerea Pers. Fr. and Rhizopus stolonifer (Ehrenb.: Fr.) Vuill. in strawberries.Ind Crops Prod. 2020; 156:112884.

Lucini EI, Zunino MP, Lopez ML, Zygadlo JA. Effect of monoterpenes on lipid composition and sclerotial development of Sclerotium cepivorum Berk. J Phytopathol. 2006: 154:441–446.

Zhang Z, Vriesekoop F, Yuan Q, Liang H. Effects of nisin on the antimicrobial activity of d-limonene and its nanoemulsion. Food Chem. 2014; 150:307–312.

Escamilla-García M, Calderon-Domínguez G, Chanona-Perez JJ, MendozaMadrigal AG, Di Pierro P, García-Almend´arez BE. Physical, Structural, Barrier, and Antifungal Characterization of Chitosan–Zein Edible Films with Added Essential Oils. Int J Mol Sci. 2017: 18 (11).

Yu D, Wang J, Shao X, Xu F, Wang H. Antifungal modes of action of tea tree oil and its two characteristic components against Botrytis cinerea. J Appl Microbiol. 2015; 119(5):1253–1262.

Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review. Evid-Based Compl Altern Med.2016;2016:3012462.

Atares L and Chiralt A. Essential oils as additives in biodegradable films and coatings for active food packaging. Trends Food Sci Technol. 2016; 48:51–62.

Mbili NC, Opara UL, Lennox CL, Vries FA. Citrus and lemongrass essential oils inhibit Botrytis cinerea on ‘Golden Delicious’, ‘Pink Lady’ and ‘Granny Smith’ apples. J Plant Dis Prot. 2017; 124(5):499–511.

Sharifi R, Kiani H, Farzaneh M, Ahmadzadeh M. Chemical Composition of Essential Oils of Iranian Pimpinella anisum L. and Foeniculum vulgare Miller and their Antifungal Activity Against Postharvest Pathogens. J Essent Oil-Bear Plants. 2008; 11:514–522.

Vilela GR, de Almeida GS, D’Arce MABR, Moraes MHD, Brito JO, da Silva MFDG. Activity of essential oil and its major compound, 1,8-cineole, from Eucalyptus globulus Labill., against the storage fungi Aspergillus flavus Link and Aspergillus parasiticus Speare. J Stored Prod Res. 2009; 45:108–111.

Kazemi M. Phytochemical Composition, Antioxidant, Anti-inflammatory and Antimicrobial Activity of Nigella sativa L. Essential Oil. J Essent Oil Bear Plants. 2014; 17:1002–1011.

Derwich E, Benziane Z, Taouil R, Senhaji O, Touzani M. Aromatic Plants of Morocco: GC/MSAnalysis of the Essential Oils of Leaves of Mentha piperita. Adv Environ Biol. 2010; 4(1):80-85.

Bertoli A, Çirak C, Teixeira da Silva J. Hypericum Species as Sources of Valuable Essential Oils. In: Medicinal and Aromatic Plant Science and Biotechnology, Global Sciece Books, 2011.

Meepagala KM, Sturtz G, Wedge DE. Antifungal constituents of the essential oil fraction of Artemisia dracunculus L. var. dracunculus. J Agric Food Chem. 2020; 50:6989–6992.

Gholamnezhad J. Effect of plant extracts on activity of some defense enzymes of apple fruit in interaction with Botrytis cinerea. J Integr Agric. 2019; 18(1):115–123.

Hua L, Yong C, Zhahnquan Z, Boqiang L, Guozheng Q, Shiping T. Pathogenic mechanisms and control strategies of Botrytis cinerea causing postharvest decay in fruits and vegetables. Food Qual Saf. 2018; 2:111–119.

Bolívar-Anillo HJ, Garrido C, Collado IG. Endophytic microorganisms for biocontrol of the phytopathogenic fungus Botrytis cinerea. Phytochem Rev. 2019; 19: 721-740.



How to Cite

Kasmi, M., Diakite, A., Barrijal, S., & Essalmani, H. (2024). Chemical Composition and Antifungal Activity of Essential Oils of <i>Rosmarinus officinalis</i> L. and <i>Salvia officinalis</i> L. against <i>Botrytis cinerea Pers</i>: Tropical Journal of Natural Product Research (TJNPR), 8(1), 5897–5907. Retrieved from