Network Pharmacology and <i>In Silico</i> Investigation on <i>Saussurea lappa</i> for Viral Respiratory Diseases

http://www.doi.org/10.26538/tjnpr/v8i1.26

Authors

  • Avin A Fitrianingsih Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, East Java, 65145, Indonesia
  • Dewi Santosaningsih Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, 65145, Indonesia
  • Susanthy Djajalaksana Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, 65145, Indonesia
  • Roihatul Muti'ah Department of Pharmacy, Faculty of Medicine and Health Science, UIN Maulana Malik Ibrahim, Malang, East Java, 65151, Indonesia
  • Maria I Lusida Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, 60131, Indonesia
  • Setyawati S Karyono 8Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, 65145, Indonesia.
  • Sumarno R Prawiro Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, 65145, Indonesia

Keywords:

Viral respiratory diseases, Saussurea lappa, Network pharmacology, Molecular docking

Abstract

Respiratory viral diseases are prevalently affecting people of all ages, requiring extensive study into herbal medicine as a potential solution. Therefore, this study aimed to identify the Saussurea lappa (S. lappa) compounds and explain the molecular mechanisms against respiratory viral diseases. The molecular mechanisms of the compound against respiratory viral diseases was determined through network pharmacological methods using Cytoscape 3.10.0, GeneCards, OMIM, STRING 11.0, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The interaction of compounds with NFĸB and TNF were analyzed using molecular docking with dexamethasone as a control through PyRx Autodock Vina 9.0 and Biovia Discovery Studio. The results showed that S. lappa compounds activated defense mechanisms against viral infection, impacting genes associated with SARS-CoV-2 disease, and activating NF-κB and NRF2 signaling pathways. The molecular docking results, supporting the network pharmacology finding, indicated that the syrigaresinol compound, with several NF-ĸB binding residues, inhibited the inflammatory pathway by blocking the protein signal. Saussureamine A and C, with lower binding affinities for TNFα, showed higher effectiveness compared to dexamethasone, showing their potential to reduce inflammation. In addition, syrigaresinol and saussureamine A and C showed potential for reducing inflammation. These results showed the potential of S. lappa as an herb for defense against SARS-CoV-2.

Author Biographies

Avin A Fitrianingsih, Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, East Java, 65145, Indonesia

Department of Microbiology, Faculty of Medicine and Health Science, UIN Maulana Malik Ibrahim, Malang, East Java, 65151, Indonesia

Maria I Lusida, Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, 60131, Indonesia

Institute of Tropical Disease, Universitas Airlangga, Surabaya, East Java, 60115, Indonesi

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Published

2024-02-01

How to Cite

Fitrianingsih, A. A., Santosaningsih, D., Djajalaksana, S., Muti’ah, R., Lusida, M. I., Karyono, S. S., & Prawiro, S. R. (2024). Network Pharmacology and <i>In Silico</i> Investigation on <i>Saussurea lappa</i> for Viral Respiratory Diseases: http://www.doi.org/10.26538/tjnpr/v8i1.26. Tropical Journal of Natural Product Research (TJNPR), 8(1), 5889–5896. Retrieved from https://tjnpr.org/index.php/home/article/view/3401