Molecular Characterization of Endophytic Fungi from the Leaves of Beruwas Laut (<i>Scaevola taccada</i> (Gaertn.) Roxb.) as Antibacterial Producer


  • Nasrul Haq Biomedical Science Study Program, Postgraduate School, Universitas Hasanuddin, Makassar, Indonesia, 90245
  • Rizalinda Sjahril Department of Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia, 90245
  • Herlina Rante Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar, Indonesia, 90245
  • Muhammad N. Massi Department of Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia, 90245
  • Ika Yustisia Department of Biochemistry, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia, 90245
  • Ilhamjaya Pattellongi Department of Physiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia, 90245


Parengyodontium album, Scaevola taccada, antibacterial, Beruwas laut, endophytic fungi


Endophytic fungi live and develop in plant tissues, are symbiotic, and are host-plant friendly. These fungi generally produce secondary metabolites with anticancer, antiviral, or antibacterial properties. This study aims to identify endophytic fungi isolated from Beruwas laut leaves. The endophytic fungi were isolated from Potato Dextrose Agar (PDA), while the secondary metabolites were produced by fermentation on Maltose Yeast Broth medium, agitated for 18 days at 150 rpm. The chemical composition of the fungi was identified using TLC. The antibacterial activity was assessed against some pathogenic bacteria strains through agar diffusion and TLC-Bioautography methods, while molecular identification was conducted using PCR. The results revealed 16 chemical isolates from the endophytic fungi, among which IFDBL-15 exhibited the most potent antibacterial activity. Inhibitory zones of the isolates against Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 14990), Bacillus subtilis (ATCC 6633), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25923), and Salmonella typhi (NCTC 786) were 15.47, 14.52, 14.64, 14.10, 15.92, and 15.52 mm, respectively. The fermentation extract of the IFDBL-15 isolate contained flavonoids, alkaloids, anthraquinones, and saponins. TLC-Bioautography activity revealed four active spots. The spot that inhibited all test bacteria was identified as a flavonoid (Rf = 0.45). Molecular identification using ITS1 and ITS4 gene regions via Polymerase Chain Reaction showed that the IFDBL-15 isolate exhibited the closest relationship to the species Parengyodontium album with an identity match of 99.83%. IFDBL-15 endophytic fungi isolate has shown the potential to generate antibacterial metabolites and could be further explored as a drug lead.

Author Biographies

Nasrul Haq, Biomedical Science Study Program, Postgraduate School, Universitas Hasanuddin, Makassar, Indonesia, 90245

Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar, Indonesia, 90231

Rizalinda Sjahril, Department of Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia, 90245

Universitas Hasanuddin Hospital, Makassar, Indonesia, 90245


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How to Cite

Haq, N., Sjahril, R., Rante, H., Massi, M. N., Yustisia, I., & Pattellongi, I. (2024). Molecular Characterization of Endophytic Fungi from the Leaves of Beruwas Laut (<i>Scaevola taccada</i> (Gaertn.) Roxb.) as Antibacterial Producer: Tropical Journal of Natural Product Research (TJNPR), 8(1), 5845–5851. Retrieved from