Acute Toxicity and Lethal Dose (LD50) of Ethyl Acetate Extract of Dandang Gendis (Clinacanthus nutans L) Leaves on Mice Using the Thompson-Weil Method

http://www.doi.org/10.26538/tjnpr/v8i1.10

Authors

  • Artati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
  • Ahyar Ahmad Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
  • Abdul Karim Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
  • Sofa Fajriah Chemical Research Center, Indonesian Institute of Sciences, Puspiptek Serpong, South Tangerang, Banten, 15314, Indonesia
  • Harningsih Karim Department of Pharmacy, Yamasi School of Pharmacy, Makassar 92171, Indonesia
  • Nuradi Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Siti N. Fajriah Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Muhammad Nasir Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Ratnasari Dewi Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Ridho Pratama Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Syahida Djasang Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Rafika Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Zulfian Armah Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Zulfikar A. Hasan Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Budirman Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia
  • Aan Yulianingsih Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Ternate, Cempaka Street Kel. Tanah Tinggi Barat, Indonesia
  • Pratiwi Hasanuddin Departement of Medical Laboratory Technology, Stikes Panrita Husada Bulukumba, Kec. Gantarang, Bulukumba Regency, South Sulawesi 92561, Indonesia
  • Asriyani Ridwan Departement of Medical Laboratory Technology, Stikes Panrita Husada Bulukumba, Kec. Gantarang, Bulukumba Regency, South Sulawesi 92561, Indonesia
  • Arfiani Nur Department of Chemistry, Faculty of Science and Technology, Alauddin State Islamic University Makassar, Jalan HM Yasin Limpo, Gowa, 92113, Indonesia

Keywords:

LD50 Value, Ethyl Acetate Extract, Dandang Gendis Leaves, Acute Toxicity

Abstract

Dandang Gendis (Clinachantus nutans L.) is a plant commonly used in traditional medicine. Based on the empirical experience of people, dandang gendis leaves are believed to possess antidiabetic and antibacterial properties. This study aimed to determine the LD50 value to assess the toxicity level of the extract and the resulting toxic effects of dandang gendis leaves. The Thompson-Weil calculation method was employed in this study, utilising 25 mice divided into 5 groups of 5 mice each. Group one served as a control (Na-CMC 1%), while the other four groups were treated with graded concentrations of 2.5% w/v, 5% w/v, 10% w/v, and 20% w/v of the extract. The extract was orally administered only once at the beginning of the study period. After administration, the mice were observed at specific intervals: 0, 0.5, 1, 2, 3, 4, 12, and 24 hours. Subsequent observations were made after 24 hours, and the mice were monitored for delayed toxic effects over 14 days. From acute toxicity testing of the ethyl acetate extract of dandang gendis leaves, the LD50 value was 6.15 g/kg BW, showing the extract is not toxic at this oral dose in mice. Further research may be necessary to fully understand the potential health benefits and risks associated with dandang gendis, particularly as an antidiabetic agent.

Author Biographies

Artati, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia

Departement of Medical Laboratory Technology, Makassar Health Polytechnic, Wijaya Kusuma Raya Street No. 46 district. Rappocini Kel. Banta-Bantaeng Makassar 90222,Indonesia

Ahyar Ahmad, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia

Research and Development Centre for Biopolymers and Bioproducts, LPPM, Universitas    Hasanuddin, Makassar-90245, Indonesia

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Published

2024-02-01

How to Cite

Artati, Ahmad, A., Karim, A., Fajriah, S., Karim, H., Nuradi, … Nur, A. (2024). Acute Toxicity and Lethal Dose (LD50) of Ethyl Acetate Extract of Dandang Gendis (Clinacanthus nutans L) Leaves on Mice Using the Thompson-Weil Method: http://www.doi.org/10.26538/tjnpr/v8i1.10. Tropical Journal of Natural Product Research (TJNPR), 8(1), 5779–5784. Retrieved from https://tjnpr.org/index.php/home/article/view/3385