Effect of Cinnamomum burmannii Bark Oil on Bacterial Count, Nuclear Factor of Kappa Beta (NF-Kβ/p65), Interleukin-6, and Chest Radiography in Rattus norvegicus with Pneumonia

http://www.doi.org/10.26538/tjnpr/v8i1.18

Abstract

Pneumonia is the second most common lung infection caused by Multidrug-Resistant Klebsiella pneumoniae. This study aimed to analyze the effect of Cinnamomum burmannii Bark oil (CbBO) as an antibacterial (decreasing bacterial counts) and anti-inflammatory agent through the inhibition of Immunoreactive Score Nuclear Factor Kappa Beta (IRS NF-Kβ/p65) and Interleukin 6 (IL-6), and chest radiography/CXR analysis of male Wistar rats' model Klebsiella pneumoniae (Kp). The experimental animals were divided into seven groups consisting of normal control group (C), negative control (NC, Kp 100 µL), Treatment 1 (T1: Kp + Carboxymethylcellulose sodium 3 mL), Treatment 2 (T2: Kp + Levofloxacin 13.5 mg/200 gram BW of rats), Treatment 3-5 (T3, T4, T5: Kp+ CbBO 0.36; 0.72; and 1.44 µL/200 gram BW of rats). The treatment was given for the first, second, and third days post-inoculation. All parameters on day 4 were examined. The bacterial count, IRS NF-Kβ/p65, and IL-6 are parametric data evaluated using Kruskall Wallis and post hoc tests. The CXR, a non-parametric data, was analysed with a chi-square test. The results showed that T3 and T4 decreased in all parameters. There was a significant difference test between NC compared T4 on the bacterial count (p-value=0.033), NC compared T3 (p-value=0.05), and T5 (p-value=0.037) on the IRS NF- Kβ/p65, and T1 compared T4 (p-value=0.045) on CXR. This study concluded that CbBO doses of 0.36 and 0.72 µL/200 gram BW of rats reduce all parameters.