HPLC Profile and Different Pathways Involved in Achillea odorata-Induced Gastric Emptying and Intestinal Transit Delay

http://www.doi.org/10.26538/tjnpr/v8i1.6

Authors

  • Hind Amira Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria.
  • Hassiba Benabdallah Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria.
  • Walid Mamache Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria.
  • Fatima Benchikh Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria.
  • Roumaissa Ounis Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria.
  • Ejike O. Okpala Department of Chemistry, University of Ibadan, Nigeria
  • Smain Amira Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University of Setif 1, 19000, Algeria.
  • Mehmet Emin duru Department of Chemistry, Faculty of Science, Mugla Sitki Kocman University, Mugla, 48000, Turkey.

Keywords:

mice, intestinal transit, gastric emptying, chemical profile, decocted extract, Achillea odorata L

Abstract

The Achillea genus is often used in traditional medicine for the treatment of digestive disorders. The current research sought to understand the mechanisms of action of the decocted extract of Achillea odorata L., with a focus on its possible effects on neurotransmitters that control gastrointestinal motility, as well as how it affected intestinal transit (IT) and gastric emptying (GE). Mice were given ADE treatments at 100, 200, or 400 mg.kg-1 doses, and an hour later they were given phenol red meal. To determine the effect of the extract on IT and GE, rats were given ADE (200 mg.kg-1) in a different set of experiments while also receiving different pharmacological agents, such as atropine (3.45 mmol.kg-1), L-Nitro-N-Arginine (L-NNA) (1.36 mmol.kg-1), or indomethacin (5.58 mmol.kg-1). At doses of 100, 200, or 400 mg.kg-1, ADE showed a significant decrease in GE and IT; the corresponding values for GE were 45.62±2.69%, 42.92±4.91%, and 28.80±3.02%, respectively. and, similarly, 57.87±3.97%, 48.72±2.01%, and 42.81±3.96% for IT. These effects on GE delay and antimotility activity were mediated through the cholinergic, nitric oxide, and cyclooxygenase pathways induced by ADE. A chemical analysis of ADE using high-performance liquid chromatography coupled with a photodiode array detector (HPLC–DAD) revealed the presence of 12 phenolic acid compounds. The predominant phenolic compound identified in A. odorata was chlorogenic acid, with a concentration of 33.43±0.18 mg.g-1. These results suggest that components of A. odorata L. may have potential applications in controlling gastrointestinal motility problems, such as diarrhoea.

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Published

2024-02-01

How to Cite

Amira, H., Benabdallah, H., Mamache, W., Benchikh, F., Ounis, R., Okpala, E. O., … duru, M. E. (2024). HPLC Profile and Different Pathways Involved in Achillea odorata-Induced Gastric Emptying and Intestinal Transit Delay: http://www.doi.org/10.26538/tjnpr/v8i1.6. Tropical Journal of Natural Product Research (TJNPR), 8(1), 5759–5764. Retrieved from https://tjnpr.org/index.php/home/article/view/3371