Evaluation of antiulcer activity of the aerial parts of Caralluma dalzielii N. E. Brown in Wistar rats https://doi.org/10.26538/tjnpr/v1i4.5
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Abstract
The aerial part of Caralluma dalzielii has been used by traditional healers in the North-west Nigeria for the treatment of stomach related problems including pain in the epigastrium. The study evaluated the antiulcer activity of aqueous extract of the aerial parts of Caralluma dalzielii (AECD) in Wistar rats. Phytochemical analysis of AECD was determined. The antiulcer activity of AECD (100, 200 and 400 mg/kg) was evaluated in absolute ethanol and indomethacin-induced ulcer models in Wistar rats following established methods. In the two models, ulcer indices and percentage gastroprotection were determined. Histopathological examination of the excised rats’
stomachs was carried out. The result of the phytochemical analysis of AECD showed the presence of flavonoids, tannins, saponins, sterols, and glycosides. AECD caused a significant (p <0.05- 0.001) decrease in mean ulcer indices in absolute ethanol (83.4-100%) and indomethacin-induced (31.6-81.6%) ulcer models compared with the control. The decrease in the ulceration in the two models were dose-dependent. Histological assessment of the stomachs showed an increase in repair of the mucosal membrane as the dose of AECD increased. There was a complete repair of the mucosal membrane at 400 mg/kg in the absolute ethanol-induced model. In conclusion, the results suggest that AECD has antiulcer effect in the absolute ethanol and indomethacin-induced ulcer models and this may be due to the presence of some bioactive compounds.
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Akah PA, Onyirioha CA, Nworu CC, Ndu OO. Gastroprotective effects of the leaf extract and fractions of Fleurya aestuans l (urticaceae). Int J Health Res. 2009; 2(1):65-73.
Kurata JH, Nogawa AN. Meta-analysis of risk factors for peptic ulcer: nonsteroidal antiinflammatory drugs, Helicobacterpylori, and smoking. J Clin Gastroenterol. 1997; 24(1):2-17.
Arun M, Asha VV. Gastroprotective effect of Dodonaea viscosa on various experimental ulcer models. J Ethnopharmacol. 2008; 118(3):460-465.
Akah PA, Nwafor SV, Okoli CO, Orji I. Evaluation of antiulcer properties of Pseudocedrela kotschyi stems bark extract. Disc Inno. 2001; 13: 132-135.
Antonio MA, Souza-Brito ARM. Oral antiinflammatory antiulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnera ulmifolia (Turneracea). J Ethnopharmacol. 1998; 61: 215-228.
Akah PA, John-Afica L, Nworu CS. Gastroprotective properties of Ocimum gratissimum L. against experimental ulcers in rat. Int.J Pharmacol. 2007; 3(5): 461- 467.
Ugwah MO, Etuk EU, Bello SO, Aliero AA, UgwahOguejiofor CJ. Comparative studies of anti-ulcerogenic activities of three Nigerian medicinal plants: A preliminary evaluation. J Med Plants Res. 2013; 7(9):490-495.
Al-Harbi MM, Raza M, Ahmed MM, Afzal M, Shah AH. Gastric antiulcer and cytoprotective effect of Commiphora molmol in rats. J Ethnopharmacol. 1997; 55: 141-150.
Nwafor SV, Okoye CF. Antiulcer properties of ethanol root extract of Cissampelos mucronata. Pharm Biol. 2005; 43 (5):396-403.
Austin A, Jegadeesan M. Gastric and duodenal anti-ulcer and cytoprotective effects of Cissus quadrangularis L.variant in rats. Nig J Natural Prod Med. 2000; 6:10-14.
Oluranti AC, Michael UO, Jane UC, Ayembe NA. Ethno botanical studies of medicinal plants used in the management of Peptic ulcer disease in Sokoto State, North Western Nigeria. Int Res J Pharm Pharmacol. 2012; 2(9):225-230.
Plowes DCH. The Stapeliads of Senegal. Catcus world 2008; 26:151-158.
de Kock D, Meve U. A Checklist of Brachystelma, Ceropegia and the genera of the Stapeliad. International Asclepiad Society, UK; 2007. 125p.
Ugwah-Oguejiofor CJ, Abubakar K, Ugwah MO, Njan AA. Evaluation of the antinociceptive and anti-inflammatory effect of Caralluma dalzielii. J Ethnopharmacol. 2013; 150(3):967-972.
Burkill HM. The useful plants of west tropical Africa, ed 2. Vol. 1: families AD. Kew, Royal Botanic Gardens. 1985.
Inngjerdingen K, Nergård CS, Diallo D, Mounkoro PP, Paulsen BS. An ethnopharmacological survey of plants used for wound healing in Dogonland, Mali, West Africa. J Ethnopharmacol. 2004; 92(2):233-244.
Oyama M, Iliya I, Tanaka T, Iinuma M. Five new steroidal glycosides from Caralluma dalzielii. Helv Chim Acta. 2007;90(1):63-71.
De Leo M, De Tommasi N, Sanogo R, Autore G, Marzocco S, Pizza C, Morelli I, Braca A. New pregnane glycosides from Caralluma dalzielii. Steroids. 2005; 70(9):573-585.
National Institutes of Health. Guide for the care and use of laboratory animals. 1985.
Trease GE, Evans WC. A Text Book of Pharmacognosy. Oxford, UK: ELSB Baillere Tindal; 1987.
Harbone J B. Phytochemical methods. London: Chapman and Hall; 1993. 68p.
Hollander D, Taranawski A, Krause WJ, Gergely H. Protective effect of sucralfate against alcohol-induced gastric mucosal injury in the rat. Gastroenterol. 1985; 88: 366-374.
Kakub G, Gulfraz M. Cytoprotective effects of Bergenia ciliata Sternb, extract on gastric ulcer in rats. Phytother Res. 2007;21(12):1217-1220.
Magaji RA, Okasha MA, Abubakar MS, Fatihu MY. Antiulcerogenic and anti-secretory activity of the n-butanol portion of Syzygium aromaticum in rat. Nig J Pharm Sci. 2007; 6:119-126.
Navarrete A, Trejo-Miranda JL, Reyes-Trejo L. Principles of root bark of Hippocratea excelsa (Hippocrataceae) with gastroprotective activity. J Ethnopharmacol. 2002; 79(3):383-388
Shah AH, Khan ZA, Baig MZ, Qureshi S, Al-Bekairi AM. Gastroprotective effects of pretreatment with Zizyphus sativafruits against toxic damage in rats. Fitoterapia. 1997; 68(3):226-234.
Bigoniya P, Rana AC, Agrawal GP. Evaluation of the antiulcer activity of Wrightia tinctoria bark extract in induced acute gastric ulcers in rat. Nig J Nat Prod Med. 2006; 10(1):36-40.
Nwafor PA, Effraim KD, Jacks TW. Gastroprotectective effects of aqueous extract of Khaya senegalensis bark in indomethacin
induced ulceration in rats. W Afr J Pharmacol Drug Res. 1996; 12:46-50.
Aguwa CN, Okunji CO. Gastrointestinal studies of Pyrenacantha staudtii leaf extracts. J Ethnopharmacol. 1986;15(1):45-55.
Borrelli F, Izzo AA. The plant kingdom as a source of anti‐ulcer remedies. Phytother Res. 2000; 14(8):581-591.
Guth PH, Paulsen G, Nagata H. Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rat: effect of prostaglandin cytoprotection. Gastroenterol. 1984; 87(5):1083-1090
Rujjanawate C, Kanjanapothi D, Amornlerdpison D, Pojanagaroon S. Anti-gastric ulcer effect of Kaempferia parviflora. J Ethnopharmacol. 2005; 102(1):120-122.
Hiruma-Lima CA, Calvo TR, Rodrigues CM, Andrade FD, Vilegas W, Brito AR. Antiulcerogenic activity of Alchornea castaneaefolia: effects on somatostatin, gastrin and prostaglandin. J Ethnopharmacol. 2006; 104(1):215-224.
Kapui Z, Boer K, Rozsa I, Blasko GY, Hermecz I. Investigations of indomethacin-induced gastric ulcer in rats. Arzneim-Forsch. 1993; 43(7):767-771.
Reeves JJ, Stables R. Effects of indomethacin, piroxicam and selected prostanoids on gastric acid secretion by the rat isolated gastric mucosa. Br J Pharmacol. 1985; 86(3):677-684.
Konturek SJ, Radecki T, Piastucki I, Brzozowski T, Drozdowicz D. Gastrocytoprotection by colloidal bismuth subcitrate (De-Nol) and sucralfate. Role of endogenous prostaglandins. Gut. 1987; 28(2):201-205