Phytochemical and In vitro Antifungal Evaluation of Some Selected Leaf Extracts against Fungi Associated with Sesamum indicium (seeds) Spoilage within Dekina Local Government Area, Kogi East, Nigeria: A Molecular Approach http://www.doi.org/10.26538/tjnpr/v7i12.44

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Published: Dec 31, 2023
Keywords:
molecular detection, fungi, plant disease, Phytochemical, antifungal, Sesame seed

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Audu N. Opisa
Department of Botany, Faculty of Sciences, Federal University, Lokoja, Nigeria.
Eze C. Simon
Department of Applied Biology and Biotechnology, Faculty of applied natural sciences, Enugu State University of Science and Technology (ESUT), Enugu, Nigeria.
Kizito E. Bello
Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia.
Zakari A. David
Department of Microbiology, Faculty of Natural Science, Kogi State University (Prince Abubakar Audu University) Anyigba, Kogi State, Nigeria.
Adetunji S. Adegoke
Department of Microbiology, Faculty of Natural Science, Kogi State University (Prince Abubakar Audu University) Anyigba, Kogi State, Nigeria.
Yusuf Lamidi
Department of Microbiology, Faculty of Natural Science, Kogi State University (Prince Abubakar Audu University) Anyigba, Kogi State, Nigeria.
Azeez Zainab
Department of Microbiology, Faculty of Natural Science, Kogi State University (Prince Abubakar Audu University) Anyigba, Kogi State, Nigeria.
Adejoh Patience
Department of Microbiology, Faculty of Natural Science, Kogi State University (Prince Abubakar Audu University) Anyigba, Kogi State, Nigeria.
Abejide R. Dorcas
Department of Biotechnology, Kogi State University, Anyigba, Kogi State, Nigeria.

Abstract

Sesame (Sesamum indicum L.) is one of Nigeria's most important oil crops, effective storage is still a leading problem with the preservation of sesame. Fungal infestation of sesame is a major cause of spoilage. The present study aimed to evaluate the potential biocontrol agents of some selected plant extracts (Cymbogogon citratus, Moringa oleifera, Azadirachta indica (Neem), and Reuwolfiea vomitoria) against four sesame seed spoilage heterotrophic fungi (Fusarium monoliforme, Aspergillus flavus, Alternaria sesame, and Penicillium sp.). Infected seed samples of sesame were collected from different sesame farms within Dekina local government and its environs to isolate fungal pathogens. The in-vitro potential of the tested plant extracts was evaluated against four isolates of phytopathogenic heterotrophic fungi in an agar well diffusion method. A total of 281 heterotrophic fungi were isolated. Alternaria sesame (number of isolate (n) = 91) had the highest distribution, while Fusarium monoliforme had the lowest distribution (n = 19). Extract concentration was impassively proportional to antifungal activity across all the fungal isolates at P<0.005. The antifungal activity of some selected extracts Cymbogogon citratus, Moringa oleifera, Azadirachta indica (Neem), and Reuwolfiea vomitoria) on the fungal isolates associated with sesame seed within Kogi East and its environs showed moderate antifungal activity (mycelium growth between 20 -60% across all test isolates). It thus offers a choice alternative in the preservation and processing of sesame seed within the region and the country.

Article Details

How to Cite
Opisa, A. N., Simon, E. C., Bello, K. E., David, Z. A., Adegoke, A. S., Lamidi, Y., Zainab, A., Patience, A., & Dorcas, A. R. (2023). Phytochemical and In vitro Antifungal Evaluation of Some Selected Leaf Extracts against Fungi Associated with Sesamum indicium (seeds) Spoilage within Dekina Local Government Area, Kogi East, Nigeria: A Molecular Approach: http://www.doi.org/10.26538/tjnpr/v7i12.44. Tropical Journal of Natural Product Research (TJNPR), 7(12), 5664-5669. https://tjnpr.org/index.php/home/article/view/3214
Issue
Vol. 7 No. 12 (2023): Tropical Journal of Natural Product Research (TJNPR)
Section
Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Kizito E. Bello, Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia.

Department of Microbiology, Faculty of Natural Science, Kogi State University (Prince Abubakar Audu University) Anyigba, Kogi State, Nigeria.

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