Cytotoxicity Testing of Gold Nanoparticles from the Aqueous Extracts of Sphagneticola trilobata (L.) J.F Pruski on Shrimp Leach and Vero Cell line http://www.doi.org/10.26538/tjnpr/v7i12.42
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Abstract
Nanotechnology in the field of biomedicine has developed rapidly in recent years. However, concerns about their safety as pharmaceutical agents prevent their widespread application. In this study, the toxicity properties were assessed for a gold nanoparticle product produced using Sphagneticola trilobata (L.) J.F. Pruski aqueous flowers and leaves extracts as a bio-reductor (AuNP-AFSt and AuNP-ALSt, respectively). Brine shrimp lethality (BSL) and Methythiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) were used for the cytotoxic assay. Toxicity on Artemia saline Leach and Vero cells were stated as Lethal Concentration (LC50) and Inhibitory Concentration (IC50), respectively. The findings showed that the LC50 values for AuNP-AFSt and AuNP-ALSt were 86.69 and 63.31 μg/mL. Additionally, the IC50 for AuNP-AFSt and AuNP-ALSt were discovered at concentrations of 30.80 μg/mL and 5.09 μg/mL. These results indicated that AuNP-AFSt and AuNP-ALSt were toxic on Shrimp leach (Meyer’s criteria), with moderate and highly toxic on Vero cells culture (NCI criteria).
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