Cytotoxicity Testing of Gold Nanoparticles from the Aqueous Extracts of <i>Sphagneticola trilobata</i> (L.) J.F Pruski on Shrimp Leach and Vero Cell line: http://www.doi.org/10.26538/tjnpr/v7i12.42

Vivi Mardina; Teuku A.  Fadlly; Tisna Harmawan; Indriaty; Nurmilasari

Authors

Vivi Mardina Department of Biology, Faculty of Engineering, Universitas Samudra, Langsa 24416.
Teuku A. Fadlly Department of Physic, Faculty of Engineering, Universitas Samudra, Langsa 24416.
Tisna Harmawan Department of Chemistry, Faculty of Engineering, Universitas Samudra, Langsa 24416.
Indriaty Department of Biology Education, Faculty of Teacher Training and Education, Universitas Samudra, Langsa 24354, Indonesia.
Nurmilasari Department of Chemistry, Universitas Syiah Kuala, Banda Aceh 23111.

Keywords:

Vero cells, Artemia saline, toxicity, Gold nanoparticle, Sphagneticola trilobata

Abstract

Nanotechnology in the field of biomedicine has developed rapidly in recent years. However, concerns about their safety as pharmaceutical agents prevent their widespread application. In this study, the toxicity properties were assessed for a gold nanoparticle product produced using Sphagneticola trilobata (L.) J.F. Pruski aqueous flowers and leaves extracts as a bio-reductor (AuNP-AFSt and AuNP-ALSt, respectively). Brine shrimp lethality (BSL) and Methythiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) were used for the cytotoxic assay. Toxicity on Artemia saline Leach and Vero cells were stated as Lethal Concentration (LC50) and Inhibitory Concentration (IC50), respectively. The findings showed that the LC50 values for AuNP-AFSt and AuNP-ALSt were 86.69 and 63.31 μg/mL. Additionally, the IC50 for AuNP-AFSt and AuNP-ALSt were discovered at concentrations of 30.80 μg/mL and 5.09 μg/mL. These results indicated that AuNP-AFSt and AuNP-ALSt were toxic on Shrimp leach (Meyer’s criteria), with moderate and highly toxic on Vero cells culture (NCI criteria).

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